Comparative studies on Actinomyces pyogenes and Arcanobacterium haemolyticum

Lämmler, Christoph; Blobel, H.

doi:10.1007/bf00189532

Cited by 37 publications

(22 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The biochemical properties of the seven A. haemolyticum strains determined with the API Coryne test According to the results of the antibiotic resistance tests of the present study, all seven A. haemolyticum strains appeared to be susceptible to enrofloxacin, erythromycin, gentamicin, penicillin G, and tetracycline, and four strains appeared to be susceptible to sulfamethoxazole-trimethoprim. The resistance of two A. haemolyticum strains to sulfamethoxazole-trimethoprim and the susceptibility to most of the other antibiotics tested corresponded to the findings of others (5,17,35).…”

Section: Resultssupporting

confidence: 73%

“…The extracellular substance causing the moderate serum liquefaction of the seven A. haemolyticum strains of the present study is not known. A cross-reaction with group G-specific antiserum could be observed, as already described (17), for the A. pyogenes control strain but not for the seven A. haemolyticum strains. The biochemical properties of the seven A. haemolyticum strains determined with the API Coryne test system are summarized in Table 2.…”

Section: Resultssupporting

confidence: 72%

See 1 more Smart Citation

Phenotypic and Genotypic Characterization of Arcanobacterium haemolyticum Isolates from Infections of Horses

et al. 2009

View full text Add to dashboard Cite

The present study was designed to characterize phenotypically and genotypically seven Arcanobacterium haemolyticum strains obtained from infections of six horses. All seven strains showed the cultural and biochemical properties typical of A. haemolyticum and were susceptible to most of the antibiotics tested. The species identification could be confirmed by amplification and sequencing of the 16S rRNA gene and the 16S-23S rRNA intergenic spacer region and by PCR amplification of species-specific parts of the gene encoding phospholipase D in A. haemolyticum. Use of the latter could possibly improve future identification of this generally human pathogenic bacterial species which, according to the present results, seems to occur also in infections of horses.Arcanobacterium haemolyticum, originally known as Corynebacterium haemolyticum, was first described in 1946 and reported to be the cause of exudative pharyngitis and skin infections in humans (23). This bacterial species, formerly considered to be a mutant form of Actinomyces (Corynebacterium) pyogenes (1), has been reclassified as a single species in the newly proposed genus Arcanobacterium (8). In the following years, the mainly animal pathogenic species Actinomyces pyogenes and human pathogenic Actinomyces bernardiae were renamed Arcanobacterium pyogenes and Arcanobacterium bernardiae, respectively, and five new species, namely, Arcanobacterium bialowiezense, Arcanobacterium bonasi, Arcanobacterium hippocoleae, Arcanobacterium phocae, and Arcanobacterium pluranimalium, were assigned to this genus (14,19,20,28). A. haemolyticum, known as a pathogen causing nonstreptococcal pharyngitis (6, 22), could also be isolated from systemic and deep-seated infections of humans. The latter was reviewed by Skov et al. (31) and Tan et al. (33). However, the isolation of A. haemolyticum from animals appears to be rare. Tyrrell et al. (34) discussed the etiological role of a single A. haemolyticum strain isolated from a periodontal infection of a rabbit.The aim of the present study was to characterize phenotypically and genotypically seven A. haemolyticum strains isolated from infections of six horses over a period of 10 years. MATERIALS AND METHODSBacterial cultures. A total of 21 bacterial cultures were used in this study. The cultures included the reference strains A. haemolyticum DSM 20595, A. bernardiae DSM 9152, A. bialowiezense DSM 17162, A. bonasi DSM 17163, A. hippocoleae DSM 15539, A. phocae DSM 10002, A. pluranimalium DSM 13483, A. pyogenes DSM 20630, Corynebacterium pseudotuberculosis biovar equi DSM 7177, and C. pseudotuberculosis biovar ovis DSM 7179 and seven bacterial cultures isolated from infections of six horses which were presumptively identified as A. haemolyticum. Further data about the origins of the seven strains are summarized in Table 1.Phenotypic properties. Hemolytic properties of the bacteria were examined on blood agar containing 5% sheep or rabbit blood after incubation of the bacteria for 24 to 48 h in a candle jar. The rabbit blood was ob...

show abstract

Section: Resultssupporting

confidence: 73%

Section: Resultssupporting

confidence: 72%

Phenotypic and Genotypic Characterization of Arcanobacterium haemolyticum Isolates from Infections of Horses

et al. 2009

View full text Add to dashboard Cite

show abstract

“…A moderate liquefaction of Loeffler medium could also be observed for the previously studied A. haemolyticum strains from horses ). However, liquefaction of Loeffler medium and cross reaction with streptococcal serogroup G-specific antisera are typical properties of A. pyogenes (Bisping and Amtsberg 1988;Lämmler and Hartwigk 1995). According to the results of the antimicrobial resistance tests presented here and corresponding to previous findings (Lämmler and Blobel 1988;Carlson et al 1994;Vargas et al 2006;Hassan et al 2009), A. haemolyticum 2289/09 appears to be susceptible to enrofloxacin, erythromycin, gentamicin, penicillin G and tetracycline and resistant to sulfamethoxazole-trimethoprim (data not shown).…”

supporting

confidence: 51%

“…In addition, A. haemolyticum 2289/09 displayed a reverse CAMP reaction with staphylococcal -hemolysin (data not shown). The hemolytic properties on rabbit blood agar, the CAMP-like reactivities and the reverse CAMP reaction are typical properties of A. haemolyticum (Lämmler and Blobel 1988;Hassan et al 2009;Ülbegi-Mohyla et al 2009). A. haemolyticum 2289/09 additionally displayed a moderate liquefaction of Loeffler medium, showed no cross-reactivity with streptococcal serogroup G-specific antisera and had typical biochemical properties of this species.…”

mentioning

confidence: 90%

Identification of Arcanobacterium haemolyticum isolated from postcastrational complications of a horse

et al. 2010

View full text Add to dashboard Cite

An Arcanobacterium haemolyticum strain isolated from a postcastrational lesion of a horse was identified phenotypically and genotypically. The latter was performed by sequencing the 16S-23S rDNA intergenic spacer region (ISR), by amplification of the gene encoding A. haemolyticum phospholipase D, by amplification of A. haemolyticum specific parts of ISR-23S rDNA and by amplification of the newly described CAMP factor family protein encoding gene of A. haemolyticum. This indicates (as described previously for seven additional A. haemolyticum strains; Hassan et al. 2009) that A. haemolyticum seems to occur also in infections of horses.

show abstract

“…After a colony is gently pushed aside, the dot is left behind as a tiny dark pit in the agar (60,130,240 Kotrajaras and Tagami (109) are consistent with A. pyogenes. The A. pyogenes reaction with antisera against group G streptococci has been suggested as a simple test for distinguishing this organism from A. haemolyticum (10,112,113); however, commercial streptococcal reagents have not yet been tested against A. pyogenes. The lack of PLD production by A. pyogenes can also distinguish it from A. haemolyticum (209), but this test has not been included in published identification schemes (35,87).…”

Section: Fluorescent Diphtheroidsmentioning

confidence: 99%

Coryneform bacteria in infectious diseases: clinical and laboratory aspects

1990

View full text Add to dashboard Cite

Coryneform isolates from clinical specimens frequently cannot be identified by either reference laboratories or research laboratories. Many of these organisms are skin flora that belong to a large number of taxonomic groups, only 40% of which are in the genus Corynebacterium. This review provides an update on clinical presentations, microbiological features, and pathogenic mechanisms of infections with nondiphtheria Corynebacterium species and other pleomorphic gram-positive rods. The early literature is also reviewed for a few coryneforms, especially those whose roles as pathogens are controversial. Recognition of newly emerging opportunistic coryneforms is dependent on sound identification schemes which cannot be developed until cell wall analyses and nucleic acid studies have defined the taxonomic groups and all of the reference strains within each taxon have been shown by molecular methods to be authentic members. Only then can reliable batteries of biochemical tests be selected for distinguishing each taxon.

show abstract

Comparative studies on Actinomyces pyogenes and Arcanobacterium haemolyticum

Cited by 37 publications

References 23 publications

Phenotypic and Genotypic Characterization of Arcanobacterium haemolyticum Isolates from Infections of Horses

Phenotypic and Genotypic Characterization of Arcanobacterium haemolyticum Isolates from Infections of Horses

Identification of Arcanobacterium haemolyticum isolated from postcastrational complications of a horse

Coryneform bacteria in infectious diseases: clinical and laboratory aspects

Contact Info

Product

Resources

About