Comparative Studies on Anti-Ag Sera in Immunodiffusion
and in Passive Hemagglutination Methods

Bütler, R.; Brunner, E.; Vierucci, A.; Morganti, G.

doi:10.1159/000466472

Cited by 18 publications

(26 citation statements)

References 5 publications

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“…Suffice it to empha size that the specimens of serum remained frozen (about -15 C) until they were thawed and sub-di vided for Ag typing at the Central Laboratory of the Blood Transfusion Service of the Swiss Red Cross in Berne. The sera were typed in duplicate, using the passive haemagglutination inhibition method 12,3], Whereas the majority of the Ne groid specimens was tested for the Ag(x) and Ag(y) specificities only, the sample of Indian as well as a sub-sample of Negroid serum specimens was tested for (he near-complete panel of the nine common Ag specificities, i.e.. Ag(x). Ag(y), A g fa ,), Ag(d), Ag(c), Ag(g), Ag(t), Ag(z), and Ag(h) (i.e.…”

Section: Samples and Methodsmentioning

confidence: 99%

A Population Genetic Study on the Ag Polymorphism in South African Indians and Negroids

Hitzeroth¹,

Bütler²,

Butler-Brunner³

1980

Hum Hered

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Two population samples from South Africa, i. e. 555 Bantu-speaking Negroids and 75 Indians, were tested for selected immunogenetic specificities comprising the Ag polymorphism. Within the Negroid sample a new Ag phenotype (i. e. the 49th) was discovered. Interpopulational comparisons involving the present two, as well as three previously studied samples, reveal characteristic differences in regard to the frequency distribution of Ag phenotypes, to the Ag allele frequencies and to the occurrence of Ag haplotypes. These results are discussed. All the data could be interpreted satisfactorily in terms of the genetic model regarding the Ag system as postulated by Bütler and his co-workers.

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Section: Samples and Methodsmentioning

confidence: 99%

A Population Genetic Study on the Ag Polymorphism in South African Indians and Negroids

Hitzeroth¹,

Bütler²,

Butler-Brunner³

1980

Hum Hered

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“…For the present investigation, ali quots were sent from Porto Alegre to Berne under refrigeration, where they arrived in good condi- tion. There they were converted into sera by clot ting and typed using the passive hemagglutination/inhibition method described previously [6], The monospecific reagents used are indicated in table I. Despite the occurrence of some nonspecif ic reactions, typing could be performed in the ma jority of the samples sent.…”

Section: Methodsmentioning

confidence: 99%

Ag Groups of the Krahó Indians of Brazil

Salzano¹,

Butler-Brunner²,

Bütler³

1981

Hum Hered

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A total of 73 sera obtained in two villages of the Krahó Indians of Brazil were tested for nine Ag factors. They were uniformly (x–y+), (a₁–d+), (t+z–) and (h–) but showed polymorphism at the cg genetic site, the frequency of Ag^g being 0.63. Considering all the antigens together, the three arrangements found are not new, but their frequencies are different from those observed in one Caucasian, one Oriental and one African population.

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“…16 A representative assay for Ag(x) will be briefly described: Ag(x + ) LDL were chemically coupled to red blood cells, which were then agglutinated with anti-Ag(x + ) antiserum in microtiter plates. If an unknown serum was added, agglutination was inhibited if the unknown LDL were Ag(x +).…”

Section: Determination Of Ag Phenotypesmentioning

confidence: 99%

Apolipoprotein B gene haplotypes. Association between Ag and DNA polymorphisms.

Ladias

Bütler

et al. 1988

Arteriosclerosis

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F ive distinct polymorphic sites on the structural gene for human apolipoprotein B (apo B) are responsible for the major antigenic polymorphisms of plasma low density lipoproteins (LDL); together, these epitopic sites constitute the system of Ag determinants. 123 Since each of the five sites encodes two alternative amino acid sequences, there exist a total of ten Ag epitopes associated with the apo B potypeptkje which may be arranged in five allelic pairs: Ag(c/g), Ag(a1/d), Ag(h/i), Ag(x/y), and Ag(t/z). Recently an association was reported between a restriction fragment length polymorphism (RFLP) detected by restriction enzyme Xba I and the Ag(c/g) epitopic site. 4 The phenotypes predicted with the Xba I RFLP were identical to those predicted with the Ag(g/c) determinant in 14 of the 17 cases examined (p<0.001). On the other hand, the same RFLP was reportedly significantly (p<0.03) associated with a different Ag locus, Ag(x/y), in a separate study involving 75 unrelated individuals that was conducted by Berg et al. We have performed a more extensive study on the relationship between the Xba I RFLP and the two Ag sites, Ag(c/g) and Ag(x/y) using 106 individuals. In this communication, we report that the Xba I RFLP of apo B is associated with both the Ag(c/g) and the Ag(x/y) sites. All of theXbal(X1/X1) phenotypes examined in this study are Ag(y/y), while most of the Xba I(X2) alleles appear to be Ag(g). We also show that these data are explained by the existence of four major alleles associated with human apo B, although other minor alleles must exist.Lending additional interest are studies providing evidence both for and against an association between human apo B polymorphisms and serum lipid and apo B levels and myocardial infarction. 6 -12 In the studies reported here, however, no statistically significant differences in lipid levels were found to be associated with any of the apo B alleles. Methods Study PopulationThe Johns Hopkins University Coronary Artery Disease (JHU-CAD) Study is a consecutive case study of men ages 50 years or less and of women ages 60 years or less who were undergoing elective nonemergency coronary arteriography at The Johns Hopkins Hospital. The spouses of the index cases were also studied. In all, 47 male index cases, 29 female index cases, 6 male spouses, and 24 female spouses were included in our sample. The details of the study will be published elsewhere, but the pertinent information is briefly summarized here. After informed consent, each index case had a medical and family history and brief physical examination. After an overnight fast of at least 12 hours,

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Comparative Studies on Anti-Ag Sera in Immunodiffusion and in Passive Hemagglutination Methods

Cited by 18 publications

References 5 publications

A Population Genetic Study on the Ag Polymorphism in South African Indians and Negroids

A Population Genetic Study on the Ag Polymorphism in South African Indians and Negroids

Ag Groups of the Krahó Indians of Brazil

Apolipoprotein B gene haplotypes. Association between Ag and DNA polymorphisms.

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Comparative Studies on Anti-Ag Sera in Immunodiffusion and in Passive Hemagglutination Methods

Cited by 18 publications

References 5 publications

A Population Genetic Study on the Ag Polymorphism in South African Indians and Negroids

A Population Genetic Study on the Ag Polymorphism in South African Indians and Negroids

Ag Groups of the Krah&oacute; Indians of Brazil

Apolipoprotein B gene haplotypes. Association between Ag and DNA polymorphisms.

Contact Info

Product

Resources

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Ag Groups of the Krahó Indians of Brazil