Comparative Studies on Chemical Contents and Effect in Kidney-Yang Deficiency Rats of Salt-Processed Product and Wine-Processed Product of Cuscutae Semen

Abstract

Cuscutae Semen mainly includes salt-processed product (SPP) and wine-processed product (WPP), which are most commonly used in traditional Chinese medicine. However, the differences between SPP and WPP have not been reported. In this paper, comparative studies between SPP and WPP on chemical contents and effect in kidney-yang deficiency rats have been investigated. UPLC-MS/MS was used to study the differences in chemical components. Kidney-yang deficiency was induced by hydrocortisone in rats. Rats were orally administrated with different doses of Jinkui Shenqi Pills, SPP, and WPP for 28 days. The average organ coefficients, renal function indexes, sex hormone levels, and testicular morphology were detected. As a result, the contents of flavonoids and chlorogenic acids were higher in SPP than in WPP, which may be caused by different processing methods. The improvement on reproduction of SPP was reflected in organ coefficients, renal function indexes, and biochemical properties of seminal plasma; furthermore, WPP was in sex hormone levels and morphology of testis. As a conclusion, these results indicated that both SPP and WPP can improve the reproductive function of kidney-yang deficiency rats with different mechanisms, which may be due to the differences in chemical contents between WPP and SPP as well as different processing methods. It is the first time that the differences between SPP and WPP have been studied in reproductive function in rats with kidney-yang deficiency.

Shudi Erzi San relieves ovary aging in laying hens

“…LBP alleviated oxidative stress and hormone secretion disorder induced by diethylstilbestrol ( DES ) in adult male hamsters ( Zhang et al, 2014 ). C. chinensis extracts increased levels of sex hormones in rats (FSH and E2 levels increased; Zhang, et al, 2019 ), which is consistent with our results. Our findings suggest that SES promotes hormone secretion and may be significant in maintenance of laying hens.…”

Yin/Yang associated differential responses to Psoralea corylifolia Linn. in rat models: an integrated metabolomics and transcriptomics study

“…The changes in body weight were recorded before the end of the experiment. From the 8th day of administration, the Yang syn and Yang syn +BGZ group rats were given 25 mg/kg hydrocortisone subcutaneously once a day for 14 consecutive days to prepare the Yang syn model (Zhang et al, 2019). From the 15th day of administration, the Yin syn and Yin syn +BGZ group rats were given thyroxine (16 mg/ml) and reserpine (1 mg/ml) at the dosage of 0.5 ml/100 g via gastric perfusion once a day for 7 consecutive days to prepare the Yin syn model (Wang, P. et al, 2010).…”

“…Continuously administered high doses of exogenous glucocorticoid (GC) (e.g., hydrocortisone) (Zhang et al, 2019) or thyroxine + reserpine (Wang, P. et al, 2010) in rats results in symptoms similar to Yang syn or Yin syn , respectively, and play important roles in the evaluation of protective or damaging effects and the TCM action mechanism against Yang syn or Yin syn . With a good description of the changes in the metabolic characteristics of endogenous metabolites and the differential expression changes of a series of functional genes in organisms, metabolomics and transcriptomics have been successfully applied to screen various diseases and their metabolic or gene pro le changes in drug intervention (Lu et al, 2020;Ren et al, 2016).…”

Ultra‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry–based plasma metabolomics study of hepatoprotective effect of cuscutae semen on CCl4‐induced liver injury model of rats

“…Shennong's Classic of Materia Medica recorded it as an upper-grade drug. Modern studies have indicated that CS contains flavonoids, polysaccharides, volatile oils, alkaloids, steroids, lignans, and so on; moreover, flavonoids and polysaccharides are the major bioactive components among them (Gao et al, 2018(Gao et al, , 2020Yang et al, 2017;Zhang et al, 2019). Currently, CS has demonstrated many pharmacological functions and biological activities, for instance, antioxidation effect (Lin et al, 2018;Szeto et al, 2012), anti-inflammatory effect (Liao et al, 2014), hepatoprotective activity (Yen et al, 2007(Yen et al, , 2008, and reversal of hormone levels (Yang et al, 2008).…”