Comparative studies on Ophiopogonis and Liriopes based on the determination of 11 bioactive components using LC–MS/MS and Hierarchical clustering analysis

Wu, Yin; Dong, Zhanjun; Wu, Huizhen; Ding, Weijing; Zhao, Mingwei; Shi, Qing; Wang, Qiao

doi:10.1016/j.foodres.2014.01.004

Cited by 26 publications

(21 citation statements)

References 8 publications

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“…High precision (RSD < 7.3% for intraday and interday variations) and acceptable recoveries (94.5–105.2%) were obtained. Finally, contents of eight homoisoflavonoids (0.07‒3.32 μg/g) in O. japonicus were determined (Supporting Information Table S2), and the obtained contents of methylophiopogonanone A and methylophiopogonanone B have good agreement with previously reported [32].…”

Section: Resultssupporting

confidence: 84%

“…Methylophiopogonanone A ( 28 ) and methylophiopogonanone B ( 33 ) are two major homoisoflavonoids in O. japonicus , therefore, researches have focused on their quantification [32]. Here, eight commercial homoisoflavonoids ( 11 , 21 , 27 , 28 , 30 , 33 , 35 , and 36 ) were selected for quantification by HPLC–QTOF‐MS using extracted ion chromatography.…”

Section: Resultsmentioning

confidence: 99%

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Homoisoflavonoids profiling of Ophiopogon japonicus by off‐line coupling high‐speed countercurrent chromatography with high‐performance liquid chromatography–diode array detector‒quadrupole time‐of‐flight tandem mass spectrometry

Deng

Tong

et al. 2020

J of Separation Science

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Roots of Ophiopogon japonicus have been used as a functional food ingredient and traditional Chinese medicine for a long time in China. Homoisoflavonoids are one of the major kinds of bioactive compounds in O. japonicus; however, literature data about its homoisoflavonoids profile are scarce because of the complex ingredients with low abundance. Here, homoisoflavonoid fraction was prepared by petroleum ether extraction. Then, a high-speed countercurrent chromatography offline coupling with high-performance liquid chromatography-diode array detectorquadrupole time-of-flight tandem mass spectrometry was developed for systematic identification of homoisoflavonoids. After that, 39 homoisoflavonoids, including 29 homoisoflavanone and 10 homoisoflavone, were unambiguously or tentatively identified, while 12 of them were reported in O. japonicus for the first time. Finally, eight available homoisoflavonoids were sensitively, precisely, and accurately determined by standard calibration curves, with limit of detection and limit of quantification in the range of 0.05-0.30 μg/mL and 0.12-0.66 μg/mL, relative standard deviation less than 7.3% for intra-and interday variations, and recovery at 94.5-105.2%. Collectively, our developed method is efficient, reliable, and valuable to profile chemical components of complex natural products.

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Homoisoflavonoids profiling of Ophiopogon japonicus by off‐line coupling high‐speed countercurrent chromatography with high‐performance liquid chromatography–diode array detector‒quadrupole time‐of‐flight tandem mass spectrometry

Deng

Tong

et al. 2020

J of Separation Science

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“…The output of various mass spectrometric methods of small molecule profiling has also been similarly analyzed with varying results. Examples include multivariate statistical analysis of data generated using: 1) Curie Point pyrolysis mass spectrometry for classification of bacteria 52 ; (2) paper spray mass spectrometry for determination of the geographic origin of coffee 53 ; (3) HPLC-tandem MS of herbal medicines to determine country of origin 54 ; (4) Ultraperformance liquid chromatography-time of flight mass spectrometry for classification of wheat lines 55 ; (5) LC-MS/MS for the assessment of the utility of using bioactive components as the basis of distinguishing between herbal medicines 56 ; (6) RPLC ESI-MS for standardization of Ginkgo biloba extracts 57 ; (7) direct injection electrospray MS for classification of coffee trees 58 ; (8) ion molecule reaction mass spectrometry for bacterial species differentiation 59 ; (9) GC- and atmospheric pressure photoionization (APPI) MS for classification of natural resins 60 ; (10) GC-GC TOF/MS for characterization and authentication of edible oils 61 , and (11) pyrolysis GC-MS profiling of eucalypt emissions in response to climate change and wildfires 62 , among other examples. The method described here differs from those outlined in the aforementioned studies in that in general, data acquisition is simpler, a broad range of compounds spanning the dielectric constant spectrum can be detected in a single experiment, and the entire information content of the observed DART-MS-derived chemical fingerprints is subjected to unsupervised hierarchical clustering (rather than using a subset of feature masses and/or chromatographic peaks).…”

Section: Discussionmentioning

confidence: 99%

A High Throughput Ambient Mass Spectrometric Approach to Species Identification and Classification from Chemical Fingerprint Signatures

Musah

Espinoza

Cody

et al. 2015

Sci Rep

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A high throughput method for species identification and classification through chemometric processing of direct analysis in real time (DART) mass spectrometry-derived fingerprint signatures has been developed. The method entails introduction of samples to the open air space between the DART ion source and the mass spectrometer inlet, with the entire observed mass spectral fingerprint subjected to unsupervised hierarchical clustering processing. A range of both polar and non-polar chemotypes are instantaneously detected. The result is identification and species level classification based on the entire DART-MS spectrum. Here, we illustrate how the method can be used to: (1) distinguish between endangered woods regulated by the Convention for the International Trade of Endangered Flora and Fauna (CITES) treaty; (2) assess the origin and by extension the properties of biodiesel feedstocks; (3) determine insect species from analysis of puparial casings; (4) distinguish between psychoactive plants products; and (5) differentiate between Eucalyptus species. An advantage of the hierarchical clustering approach to processing of the DART-MS derived fingerprint is that it shows both similarities and differences between species based on their chemotypes. Furthermore, full knowledge of the identities of the constituents contained within the small molecule profile of analyzed samples is not required.

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“…However, cluster B could be further divided into two subgroups (B1 and B2). Sample numbers 1, 3,6,7,9,14,18,21,24, and 25 were in subgroup B1, and the remaining cluster B samples were in subgroup B2. From the plot, it is evident that the Hainan samples (S8 and S11) are clustered in A, which distinguishes these samples from the others.…”

Section: Hierarchical Clustering Analysismentioning

confidence: 99%

Evaluation and quantitative analysis of 11 compounds in Morinda officinalis using ultra‐high performance liquid chromatography and photodiode array detection coupled with chemometrics

Zhao

Kong

Zhou

et al. 2017

J of Separation Science

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Morinda officinalis (Rubiaceae) is a traditional Chinese medicine widely used for the treatment of impotence and osteoporosis in clinical therapy. In the present study, a rapid and simple ultra-high performance liquid chromatography with photodiode array detection method was developed and validated for the simultaneous determination of 11 bioactive compounds in M. officinalis. This assay method was validated with respect to linearity (R > 0.9991), precision, repeatability, limit of detection, limit of quantification, and accuracy (with observed recovery rates between 94.21 and 100.38%). The quantitative results revealed significant differences in the concentrations of the selected compounds. Additionally, chemometric methods, including hierarchical clustering analysis, principal component analysis, and partial least-squares discriminate analysis, were applied to compare and sort the 25 batches of M. officinalis samples based on the quantitative data of the analytes. All of the samples were clearly divided into two groups: the Hainan samples were successfully discriminated from the samples from other origins. Simultaneous determination of multiple compounds using the proposed method combined with chemometrics could be a viable strategy to compare and evaluate the quality of M. officinalis.

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Comparative studies on Ophiopogonis and Liriopes based on the determination of 11 bioactive components using LC–MS/MS and Hierarchical clustering analysis

Cited by 26 publications

References 8 publications

Homoisoflavonoids profiling of Ophiopogon japonicus by off‐line coupling high‐speed countercurrent chromatography with high‐performance liquid chromatography–diode array detector‒quadrupole time‐of‐flight tandem mass spectrometry

Homoisoflavonoids profiling of Ophiopogon japonicus by off‐line coupling high‐speed countercurrent chromatography with high‐performance liquid chromatography–diode array detector‒quadrupole time‐of‐flight tandem mass spectrometry

A High Throughput Ambient Mass Spectrometric Approach to Species Identification and Classification from Chemical Fingerprint Signatures

Evaluation and quantitative analysis of 11 compounds in Morinda officinalis using ultra‐high performance liquid chromatography and photodiode array detection coupled with chemometrics

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