Comparative studies on pheno- and genotypic properties of Staphylococcus aureus isolated from bovine subclinical mastitis in central Java in Indonesia and Hesse in Germany

Salasia, Siti Isrina Oktavia; ., Khusnan; Lämmler, Christoph; Zschöck, M.

doi:10.4142/jvs.2004.5.2.103

Cited by 81 publications

(82 citation statements)

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“…In the present study, among the coagulase positive Staphylococcus isolates recovered, S. aureus isolates were predominant (88.23%). A similar finding was also recorded in several studies (Salasia et al 2004;Kalorey et al 2007). …”

Section: Discussionsupporting

confidence: 82%

Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk

Chavhan

Nagdive

Purohit

et al. 2011

Trop Anim Health Prod

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Biofilm formation in Staphylococcus aureus is considered an important virulence factor in bovine mastitis. Intercellular adhesion gene A (icaA) is a significant genetic determinant that contributes in biofilm formation. The aim of the present study was to determine the presence of the icaA gene in S. aureus isolated from bovine mastitis from seven states of India. A total of 88 out of 150 Staphylococcus aureus strains were found to be positive for biofilm marker icaA gene by PCR. The icaA gene was confirmed by dot blot hybridization in 41 of 150 S. aureus strains tested. Results obtained with dot blot hybridization were comparable to those obtained with PCR. Partial sequences of the icaA gene of the two S. aureus isolates showed deletion of some bases in different positions that might reduce/stop transcription leading to no biofilm formation. PCR was found to be a rapid test but dot blot hybridization was more accurate than PCR for detection of icaA genes. This study showed that detection of biofilm marker the icaA gene in S. aureus would allow the detection of virulence factors present in mastitis and early application of corrective measures.

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Section: Discussionsupporting

confidence: 82%

Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk

Chavhan

Nagdive

Purohit

et al. 2011

Trop Anim Health Prod

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“…The presence of cap genes in all of the isolates analysed was also reported in the studies conducted by Tollersrud et al (2000); Salasia et al (2004); Bardiau et al (2014), and Khichar and Kataria (2014), with S. aureus isolated from bovine mastitis, as well as by Verdier et al (2007), with S. aureus isolated from human infections. Nevertheless, Reinoso et al (2008) and Camussone et al (2012) in Argentina, Babra et al (2013) in Australia and Marques et al (2013) in Brazil reported lower percentages of S. aureus isolates collected from bovine mastitis samples carrying the cap gene.…”

Section: Polysaccharide Productionsupporting

confidence: 60%

“…A higher proportion of cap5 isolates (compared to cap8 isolates) has also been reported by Salasia et al (2004) in Indonesia, Reinoso et al (2008) and Camussone et al (2012) in Argentina, and Khichar and Kataria (2014) in India. Other studies conducted in different countries reported that cap5 and cap8 were evenly distributed among S. aureus isolates from bovine mastitis (Bar-Gal et al 2015) or that there was a higher proportion of cap8 than cap5 isolates (Salasia et al 2004;Babra et al 2013;Marques et al 2013;Bardiau et al 2014).…”

Section: Polysaccharide Productionmentioning

confidence: 68%

Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms

et al. 2016

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Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.

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“…The PCR based methods have also been used extensively for molecular characterization of S. aureus in bovine mastitis (Akineden et al 2001;Salasia et al 2004;Zschöck et al 2004;El-Sayed et al 2006). Recently, Reinoso et al (2008) observed genotypic variations among the isolates of S. aureus in relation to their origin.…”

Section: Introductionmentioning

confidence: 98%

Prevalence of adhesin and toxin genes among isolates of Staphylococcus aureus obtained from mastitic cattle

Kumar

Yadav

Anand

et al. 2010

World J Microbiol Biotechnol

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Staphylococcus aureus is recognized as a major etiological agent of the most important udder disease; mastitis. The virulence potential of S. aureus isolates is attributed to a combination of extracellular factors and invasive properties that are controlled at the genomic level. In this study molecular variations were studied among the 128 S. aureus isolates obtained from the mastitic crossbred cattle. The polymorphic patterns of protein A, coagulase, and IG genes were studied in the isolates using the PCR and PCR-RFLP methods. In addition, presence of other virulence genes responsible for the expression of adhesins and toxins was also screened. A considerable genetic variation was observed in amplified fragment of SPA, IG and CLF genes. The PCR-RFLP of COA gene yielded five different patterns. Occurrence of EBP, ENO and FNBA was found to be common in both high and low virulence isolates. However, the prevalence of FNBB and toxin genes was higher in clinical than subclinical isolates. The distribution proportions of adhesin genes EBP, FIB, FNBB, CNA, BBP, MAP CAP5, and CAP8 were 60.2, 59.4, 21.9, 6.3, 5.5, 80.5, 56.3, and 22.7%; for AGR I, II, III, and IV were 44.5, 32.8, 12.5, and 10.2%; and for toxins genes HLB, SEB, SEC, SED, SEE, SEG, and SEI were 82.0, 3.1, 5.5, 3.9, 0.8, 16.4, and 55.5%, respectively. The proportions of FNBB, EBP, FIB, HLB, BBP, SEG, and SEI genes were observed more in clinical cases. The significant genetic variations in SPA, COA, IG, and CLF genes were useful to differentiate the isolates that might be valuable for mastitis reduction programme.

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Comparative studies on pheno- and genotypic properties of Staphylococcus aureus isolated from bovine subclinical mastitis in central Java in Indonesia and Hesse in Germany

Cited by 81 publications

References 0 publications

Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk

Molecular characterization of intercellular adhesion gene in Staphylococcus aureus isolated from bovine mastitic milk

Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms

Prevalence of adhesin and toxin genes among isolates of Staphylococcus aureus obtained from mastitic cattle

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