Comparative studies on the interaction of nitrofuran antibiotics with bovine serum albumin

Zhang, Qiulan; Ni, Yongnian

doi:10.1039/c7ra05570f

Cited by 22 publications

(6 citation statements)

References 40 publications

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“…It is clearly seen that the characteristic emission band of BSA (due to the tryptophan residue) decreases progressively during each titration, which supports the formation of the metal compound‐BSA adducts. In addition, a minor blue shift accompanied the latter spectral alterations within the BSA fluorescence spectra, which is synonymous of the tryptophan residues being enclosed in a hydrophobic pocket due to increased folding of the BSA strand (consistent with the results in the UV‐Vis study) [36a,40] …”

Section: Resultssupporting

confidence: 84%

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Biomolecular Interactions of Cytotoxic Ruthenium Compounds with Thiosemicarbazone or Benzothiazole Schiff Base Chelates

et al. 2022

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Herein we illustrate the formation and characterization of new paramagnetic ruthenium compounds, trans-P-[RuCl(PPh 3 ) 2 (pmt)]Cl(1)Agarose gel electrophoresis experiments of the metal compounds illustrated dose-dependent binding to gDNA by 1-3, while methylene blue competition assays suggested that 1 and 2 are also DNA intercalators. Assessment of the effects of the compounds on topoisomerase function indicated that 1-3 are capable of inhibiting topoisomerase I activity in terms of the ability to nick supercoiled plasmid DNA. The cytotoxic activities of the metal complexes were determined against a range of cancer cell lines versus a nontumorigenic control cell line, and the complexes were, in general, more cytotoxic towards the cancer cells, displaying IC 50 values in the low micromolar range. Time-dependent stability studies showed that in the presence of strong nucleophilic species (such as DMSO), the chloride co-ligands of 1-3 are rapidly substituted by the former as proven by the suppression of the substitution reactions in the presence of an excess amount of chloride ions. The metal complexes are significantly stable in both DCM and an aqueous phosphate buffer containing 2 % DMSO.

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Section: Resultssupporting

confidence: 84%

“…In addition, a minor blue shift accompanied the latter spectral alterations within the BSA fluorescence spectra, which is synonymous of the tryptophan residues being enclosed in a hydrophobic pocket due to increased folding of the BSA strand (consistent with the results in the UV‐Vis study). [ 36a , 40 ]…”

Section: Resultsmentioning

confidence: 99%

Biomolecular Interactions of Cytotoxic Ruthenium Compounds with Thiosemicarbazone or Benzothiazole Schiff Base Chelates

et al. 2022

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“…Veterinary drug residues have attracted significant attention following clenbuterol poisoning. The docking results of furan antibiotics nitrofurantoin (NFT) and nitrofurazone (NFZ) with BSA showed that the binding sites were located in the hydrophobic cavity of BSA, while electrostatic and hydrophobic interactions featured prominently during the binding process (Zhang & Ni, 2017). The directional mutagenesis of amino acid residues was performed on the anti-sarafloxacin ScFv antibody, and then, the affinity of the mutant antibodies to the drug was evaluated with molecular docking (Wang et al, 2016a).…”

Section: Food Safety Hazard Factorsmentioning

confidence: 99%

Recent developments in molecular docking technology applied in food science: a review

Tao

Huang

Wang

et al. 2019

Int J of Food Sci Tech

150

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Molecular docking is a theoretical simulation method based on bioinformatics, which studies the interaction between molecules (such as ligands and receptors), and predicts their binding modes and affinity via a computer platform. This technology acts as a promising mean in medicinal chemistry such as structurebased rational drug design, which is accepted by researchers in the scientific community. During recent years, various fundamental studies involving biomolecular interaction in the food matrix have gradually emerged. The remarkable advantages of molecular docking such as predicting experiments are attracting increasing attention for its application potential in various fields. This review presents the theory and software development of molecular docking, and emphasises its application in the field of food science, including nutritional components and food safety. Moreover, the operational mechanisms of molecular docking are further summarised in this review.

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“…BSA interactions with small molecules have become increasingly important in pharmacochemistry and are commonly used as key steps in the construction of medicinal compounds [17][18][19][20][21]. The research in this field provides strong support for BSA-binding studies and a deeper understanding of the way medicaments target and bind receptors [22,23].…”

Section: Introductionmentioning

confidence: 99%

BSA-binding studies of 2- and 4-ferrocenylbenzonitrile: voltammetric, spectroscopic and molecular docking investigations

Benamara

Lanez

2020

J. Electrochem. Sci. Eng.

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The binding affinity of 2-ferrocenylbenzonitrile (2FBN) and 4-ferrocenylbenzonitrile (4FBN) with bovine serum albumin (BSA) has been investigated by cyclic voltammetry, absorption spectroscopy and molecular modelling techniques. The results indicated that both of the two derivatives could bind to BSA and cause conformational changes with the order 2FBN > 4FBN. The voltammetric behavior of 2FBN and 4FBN before and after the addition of the BSA suggests that the redox process is kinetically controlled by the diffusion step, and demonstrated that the diffusion coefficients of the 2FBN-BSA and 4-FBN-BSA adducts are lower than that of the free compounds. Furthermore, molecular docking suggested that the binding mode of the two compounds to BSA is of hydrophobic and hydrogen bond interactions, moreover the ligand 2FBN additionally show a π-cation interaction.

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Comparative studies on the interaction of nitrofuran antibiotics with bovine serum albumin

Abstract: The study indicated that the nitrofurazone (NFZ) offered stronger toxicity than nitrofurantoin (NFT) in the interactions with albumin.

Cited by 22 publications

References 40 publications

Biomolecular Interactions of Cytotoxic Ruthenium Compounds with Thiosemicarbazone or Benzothiazole Schiff Base Chelates

Biomolecular Interactions of Cytotoxic Ruthenium Compounds with Thiosemicarbazone or Benzothiazole Schiff Base Chelates

Recent developments in molecular docking technology applied in food science: a review

BSA-binding studies of 2- and 4-ferrocenylbenzonitrile: voltammetric, spectroscopic and molecular docking investigations

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