Comparative studies on the production of statins using different microbial strains

Abstract

In this study, we aimed to increase the yield of mevastatin and lovastatin in solid state fermentation (SSF) using three different fungi, Aspergillus terreus MTCC 279, Penicillium citrinum MTCC 1751, and Penicillium brevicompactum MTCC 549. Initially, we screened various substrates for maximizing the production of mevastatin and lovastatin, and barley powder was found to be the most suitable substrate for A. terreus. We applied response surface methodology (RSM) to determine the optimal parameters for initial moisture content, temperature, and inoculum size. The use of RSM resulted in maximizing the production of mevastatin to 288.13 mg/gram dry substrate (gds) and lovastatin to 329.51 mg/gds. For further studies, we used the high yielding strain of A. terreus with barley as a substrate. In the validation experiment, the maximum amount of mevastatin (297.98 mg/gds) and lovastatin (340.71 mg/gds) was obtained using A. terreus with barley as a substrate. This is the first report on the simultaneous production of mevastatin and lovastatin using a high yielding strain in SSF.

Diversity and bioactivity of fungi associated with the marine sea cucumber Holothuria poli : disclosing the strains potential for biomedical applications

“…To address the microbe as the metabolite producer instead of an animal or plant is very advantageous: once isolated, a fungus can be grown in vitro , allowing further studies with no more expensive and environmental‐impacting sampling campaigns. Moreover, the development of fermenters for fungal cultivation allows industrial production and extraction of the metabolite from the biomass (Syed 2019).…”

Diversity and characterization of culturable fungi associated with the marine sea cucumber Holothuria scabra

“…Exploiting microorganisms as metabolite and enzyme producers is very advantageous because a fungus can be cultivated in vitro , allowing further studies to be conducted without environmental impact. Moreover, the development of fermentation processes for fungal cultivation allows the industrial production and extraction of metabolites [ 24 ].…”

Ultrasonic Irradiation Enables Facile Production of Lovastatin from Sugar Cane Bagasse

“…Lovastatin is a potent competitive inhibitor of the rate-limiting enzyme in the cholesterol biosynthesis of 3-hydroxy-3-methyl glutaryl coenzyme A (HMG-CoA) reductase. 1 Lovastatin can be produced from numerous fungi such as Penicillium spp., 2 Aspergillus terreus , 3 and Monascus purpureus . 4 M. purpureus is a nonpathogenic fungus which has been used traditionally in China and Japan for natural pigment production along with lovastatin under solid-state fermentation (SSF).…”

“…Lovastatin is a potent competitive inhibitor of the rate-limiting enzyme in the cholesterol biosynthesis of 3-hydroxy-3-methyl glutaryl coenzyme A (HMG-CoA) reductase . Lovastatin can be produced from numerous fungi such as Penicillium spp., Aspergillus terreus, and Monascus purpureus M.…”