Comparative study and molecular characterization of ectomycorrhizas in Tilia americana and Quercus pubescens with Tuber brumale

Abstract

Mycorrhizas of Tuber brumale on Quercus pubescens and Tilia americana were obtained in vitro using micropropagated plantlets. Mycelium pure cultures were used for inoculation. Both the mycelium used for the inoculations, as well as the mycorrhizas which were obtained, were identified using several molecular approaches: analysis of the ITS region, polymerase chain reaction (PCR) specific primers and sequencing. The mycorrhizas were described from a morphological standpoint. Some of their biometric characteristics were different in bass-wood than they were in oak, thus showing the influence of the host plant on several of the morphological features believed to be necessary for the identification of the species. Considering the variability of their morphological characteristics, molecular analysis proved to be a necessary tool for the recognition of the mycorrhizas of Tuber spp.

Tilia platyphyllos Scop.–Tuber brumale Vittad. vs. T. platyphyllos Scop.–T. borchii Vittad. ectomycorrhizal systems: a comparison of structural and functional traits

“…Unfortunately, molecular information explaining how mycorrhiza formation is controlled and develops, in particular regarding Tuber species, is still limited [7,[9][10][11][12][13][14]. Ectomycorrhizal model systems have been set up by inoculating the roots of some plants with several Tuber species [15,16] in the attempt to investigate plant and mycelium modifications induced by the mycorrhization process. In this paper, the characterisation of two model systems, obtained associating Tuber brumale Vittad.…”

Truffle Phylogenomics

“…Hebeloma cylindrosporum) (Marmeisse et al, 2004), it is impossible to complete the truffle life cycle in vitro. Indeed, it is possible to obtain free-living mycelium of different Tuber spp., which grow slowly (Iotti, Amicucci, Stocchi, & Zambonelli, 2002), and ectomycorrhizae (Giomaro et al, 2002) can be produced, but to our knowledge, the sexual reproduction of Tuber has never been observed in vitro. The only documented production of ascocarps in control conditions was the fruiting of T. maculatum in vases associated with Pinus strobus (Fassi & Fontana, 1969).…”

Soil fungal communities in a Castanea sativa (chestnut) forest producing large quantities of Boletus edulis sensu lato (porcini): where is the mycelium of porcini?

“…Mycorrhizae C. sativa formed by these species are morphologically similar to each other and have the anatomo‐morphological features previously described on Cistus ladanifer by Águeda and colleagues (2006) . The only difference was the darker ochre, ochre brown colour although it is well known that this morphological character can vary with age and the species of host plant (Zak, 1973; Giomaro et al ., 2002). Detection of Boletus mycelia via specific primers using the same DNA extracts showed that its mycelia were present in the soil, but that the proportion of their DNA from the totally extracted DNA was too little to be detected by cloning techniques.…”