Comparative study for separation of aquatic humic substances by capillary zone electrophoresis using uncoated, polymer coated and gel-filled capillaries

Capillary Electrophoresis and Fluorescence Excitation‐Emission Matrix Spectroscopy for Characterization of Humic Substances

“…The CE profi les of NOM are affected by many factors, such as different buffer systems, buffer concentrations, buffer pH, ionic strength, and sample concentration (Schmitt-Kopplin et al, 1998b;Egeberg and Bergli, 2002;Schmitt-Kopplin and Kettrup, 2003;Schmitt-Kopplin and Junkers, 2003;Peuravuori et al, 2004). We applied the analytic conditions reported by Pompe et al (1996), and observed similar CE profi les of the Suwannee River HA and FA samples, which were reported in their study.…”

Recent advances in capillary electrophoresis and capillary electrochromatography of pollutants

“…Many papers were published on the real possibilities of CE in the field of HS as presented in-depth in the tabulated review by SchmittKopplin and Junkers [4]. Several studies have reported the use of CE for the fingerprinting and characterization of HS and natural organic matter (NOM) [177][178][179][180][181][182][183][184]. For example, Schmitt-Kopplin and Kettrup [177] used CZE hyphenated to ESI-MS for the separation of Suwannee River NOM.…”

Determination of limiting mobilities and dissociation constants of 21 amino acids by capillary zone electrophoresis at very low pH

“…According to [19], the limiting mobilities and thermodynamic dissociation constant for Asp m lim,A − = 30.1 × 10 −9 m 2 s −1 V −1 and pK a,2 = 3.90 and for Glu m lim,A − = 27.0 × 10 −9 m 2 s −1 V −1 and pK a,2 = 4.32 were recalculated (see Eqs. (4) and (5) The relevance of the above fitting process is based on the assumption that the actual mobilities depend only on the ionic strength of the BGEs. This assumption may not be exactly fulfilled in BGEs with significant concentration of hydroxonium ions, which exhibit the so-called mixing effects, as shown by Onsager and Fuoss [37].…”

“…The second kind -permanent coating -is formed by molecules strongly bonded to the inner capillary wall. Among all the capillary coatings described in literature, those based on poly(vinyl alcohol) (PVA) have proven to exhibit a particularly good performance [1][2][3][4][5][6]. Extraordinary high separation efficiencies could be achieved using capillaries coated with PVA, especially for proteins [2].…”