Comparative study of an estradiol enzyme-linked immunosorbent assay kit, liquid chromatography–tandem mass spectrometry, and ultra performance liquid chromatography–quadrupole time of flight mass spectrometry for part-per-trillion analysis of estrogens in water samples

Farré, Marinella; Kuster, Marina; Brix, Rikke; Rubio, Fernando; Alda, Miren López de; Barceló, Damià

doi:10.1016/j.chroma.2007.05.032

Cited by 129 publications

(72 citation statements)

References 20 publications

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“…ELISA is a test used as a general screening tool for the detection of antibodies or antigens in a sample [37]. ELISA technology links a measurable enzyme to either an antigen or antibody.…”

Section: Enzyme-linked Immunosorbent Assays (Elisa)mentioning

confidence: 99%

Review Article: Immobilized Molecules Using Biomaterials and Nanobiotechnology

Elnashar¹

2010

JBNB

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“…ELISA is a test used as a general screening tool for the detection of antibodies or antigens in a sample [37]. ELISA technology links a measurable enzyme to either an antigen or antibody.…”

Section: Enzyme-linked Immunosorbent Assays (Elisa)mentioning

confidence: 99%

Review Article: Immobilized Molecules Using Biomaterials and Nanobiotechnology

Elnashar¹

2010

JBNB

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“…For many years, immunoassay methods have been the most sensitive analytical procedures available for the determination of steroidal hormones in biological samples [9][10][11]. These methods are sensitive, but prone to cross reactivity by endogenous hormones, co-administrated hormones and their metabolites.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of 15 steroidal oral contraceptives in water using solid-phase disk extraction followed by high performance liquid chromatography–tandem mass spectrometry

Sun

Yong

Chu

et al. 2009

Journal of Chromatography A

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a b s t r a c tA rapid, accurate and sensitive method for simultaneous determination of 15 steroidal hormones including four estrogens (estrone, 17␤-estradiol, 17␣-ethynylestradiol, estriol) and eleven progestogens (17␤-estradiol-3-benzoate, 19-norethindrone, gestodene, levonorgestrel, medroxyprogesterone, cyproterone acetate, megestrol-17-acetate, progesterone, norethindrone acetate, chlormadinone-17-acetate, and hydroxy progesterone caproate) in environmental waters was developed by coupling solid-phase disk extraction (SPDE) to ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with electrospray ionization. Among three types of extraction tested (C 8 SPDE, C 18 SPDE and C 18 SPE), the most satisfactory result was achieved using C 18 SPDE for its satisfactory recovery (75.6 to 101.4%) and short extraction time (15 min for 1 L deionised water). The validity of this method was investigated and good analytical performance for all the analytes was obtained, including low limits of method detection (0.5-3.4 ng/L) and excellent linear dynamic range (1.0-50.0 ng/L). The method was applied to determine the steroidal hormones in 10 environmental waters including tap water, river water, lake water and waste water in Beijing. No progestogen was detected in all samples and estrone, estriol, 17␣-ethynylestradiol were found in most samples at levels between 1.8 and 127.9 ng/L.

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“…For analysis of 100-ml 23 sample, the method detection limits (LOD) ranged from 4.5 to 9.8 ng L -1 and the limit of 24 quantitation (LOQ) from 14.9 to 32.6 ng L -1 . systems such as UV [7,8], fluorescence [9] and coupled to mass spectrometry [10,11,12,13], 49 gas-chromatography after derivatization [14] and enzyme-linked immunosorbent assay [15]. 50…”

Section: Introductionmentioning

confidence: 99%

Solid-phase extraction using molecularly imprinted polymer for selective extraction of natural and synthetic estrogens from aqueous samples

Lucci

Núñez

Galcerán

2011

Journal of Chromatography A

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9A method is proposed for the clean-up and preconcentration of natural and synthetic estrogens 10 from aqueous samples employing molecularly imprinted polymer (MIP) as selective sorbent 11 for solid-phase extraction (SPE). The selectivity of the MIP was checked toward several 12 selected natural and synthetic estrogens such as estrone (E1), 17β-estradiol (β-E2), 17α-13 estradiol (α-E2), estriol (E3), 17α-ethinylestradiol (EE2), dienestrol (DIES) and 14 diethylstilbestrol (DES). Ultrahigh pressure liquid chromatography (UHPLC) coupled to a 15 TSQ triple quadrupole mass spectrometry (QqQ) was used for analysis of target analytes. The 16 chromatographic separation of the selected compounds was performed in less than 2 min 17 under isocratic conditions. The method was applied to the analysis of estrogens in spiked river 18 and tap water samples. High recoveries (>82%) for estrone, 17β-estradiol, 17α-estradiol, 19 estriol and 17α-ethinylestradiol were obtained. Lower but still satisfactory recoveries (>48%) 20were achieved for dienestrol and diethylstilbestrol. The method was validated and found to be 21 linear in the range 50-500 ng L -1 with correlation coefficients (R 2 ) greater than 0.995 and 22 repeatability relative standard deviation (RSD) below 8% in all cases. For analysis of 100-ml 23 sample, the method detection limits (LOD) ranged from 4.5 to 9.8 ng L -1 and the limit of 24 quantitation (LOQ) from 14.9 to 32.6 ng L -1 . systems such as UV [7,8], fluorescence [9] and coupled to mass spectrometry [10,11,12,13], 49 gas-chromatography after derivatization [14] and enzyme-linked immunosorbent assay [15]. 50Currently, liquid chromatography coupled with tandem mass spectrometry is the most 51 common approach. However, as the concentrations of the estrogenic compounds in 52 environmental matrices are very low, a clean-up and preconcentration step is usually required 53 in order to minimize interferences and improve method accuracy and sensitivity. Solid-phase 54 extraction (SPE) is a well-established method routinely used for clean-up and 55 preconcentration step of this compounds [16]. The main drawback of conventional SPE 56 sorbents is their lack of selectivity resulting in co-extraction of interfering matrix components, 57 which can negatively affect quantitation. Selectivity can be obtained using sorbents based on 58 molecularly imprinted polymers (MIP). These types of sorbents are synthetic materials 59 possessing an artificially generated three-dimensional network that is able to specifically 60 rebind a target analyte, or class of structurally related compounds. MIP has the advantages of 61 being very selective, cost-effective, and not suffering from storage limitations and stability 62 problems regarding organic solvents. MIPs have been proposed in recent years as sorbent for 63 the extraction and/or removal of endocrine disrupting compounds [17,18,19]. In addition, the 64 potential of MIP as SPE sorbent for extraction of diethylstilbestrol [20,21] Extraction and clean-up using MIP-SPE 129 1...

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Comparative study of an estradiol enzyme-linked immunosorbent assay kit, liquid chromatography–tandem mass spectrometry, and ultra performance liquid chromatography–quadrupole time of flight mass spectrometry for part-per-trillion analysis of estrogens in water samples

Cited by 129 publications

References 20 publications

Review Article: Immobilized Molecules Using Biomaterials and Nanobiotechnology

Review Article: Immobilized Molecules Using Biomaterials and Nanobiotechnology

Simultaneous determination of 15 steroidal oral contraceptives in water using solid-phase disk extraction followed by high performance liquid chromatography–tandem mass spectrometry

Solid-phase extraction using molecularly imprinted polymer for selective extraction of natural and synthetic estrogens from aqueous samples

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