Comparative study of antioxidant enzymes in tissues surrounding implant in rabbits

Özmen, İsmail; Nazıroğlu, Mustafa; Okutan, Ramazan

doi:10.1002/cbf.1225

Cited by 40 publications

(20 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An animal study of Ozmen et al [20] indicated a similar effect in vivo. It is shown that 1 month after the insertion of titanium implants in rabbits an increase in lipid peroxidation (a possible result of oxidative stress) in the tissues surrounding the titanium implants occurred, whereas there was a decrease in the activities of antioxidant enzymes (e.g., catalase, SOD, glutathione peroxidase).…”

Section: Article In Pressmentioning

confidence: 65%

Response of human endothelial cells to oxidative stress on Ti6Al4V alloy

et al. 2007

View full text Add to dashboard Cite

Section: Article In Pressmentioning

confidence: 65%

Response of human endothelial cells to oxidative stress on Ti6Al4V alloy

et al. 2007

View full text Add to dashboard Cite

“…ROS are active components of the phagocytic system of leucocytes and serve as mediators of tissue damages. 26 We investigated the effects of PLGA/HPOX blend films on the generation of ROS by macrophages to evaluate the adequacy of this material for tissue engineering applications. At a short culture time, despite its excellent biocompatibility, PLGA showed a significant increase of ROS production due probably to transitory stimulation of mitochondrial activity and oxidative stress.…”

Section: Resultsmentioning

confidence: 99%

Reduced inflammatory responses to poly(lactic-co-glycolic acid) by the incorporation of hydroxybenzyl alcohol releasing polyoxalate

et al. 2011

View full text Add to dashboard Cite

Poly(lactic-co-glycolic acid) (PLGA) has been widely used in biomedical and pharmaceutical applications because of its satisfactory biocompatibility and biodegradation profile. However, its applications for tissue engineering scaffolds have been plagued by inflammatory responses that can lead to fibrotic encapsulation. p-Hydroxybenzyl alcohol (HBA) is a phenolic compound in herbal agents that exerts antioxidant and anti-inflammatory activities. In this work, we developed PLGA blend films containing HBA-incorporated peroxalate polymer (HPOX) and investigated the effects of HPOX on the local inflammatory responses to PLGA in vitro and in vivo. The PLGA/HPOX blend films had no adverse effects on cell viability and elicited a significantly lower production of pro-inflammatory mediators such as tumor necrosis factor-α and reactive oxygen species than PLGA films. We also found that PLGA/HPOX blend films implanted into a rat had significantly diminished inflammatory responses. PLGA/HPOX blends appear to be a promising approach for tissue engineering applications due to their excellent biocompatibility and anti-inflammatory effects.

show abstract

“…Incidentally, titanium implants decreases the catalase and SOD enzyme activity in endothelial cells and tissues surrounding an implant. Moreover, this persistent ROS formation in cells on a titanium surface can exhaust the enzyme activity due to protein oxidation by ROS 28, 29…”

Section: Discussionmentioning

confidence: 99%

Enhancement of osteoblast biocompatibility on titanium surface with Terrein treatment

Lee¹,

Lee²,

Bhattarai³

et al. 2010

Cell Biochemistry & Function

View full text Add to dashboard Cite

Titanium is biocompatible with bodily tissues. However, the formation of ROS on the titanium surfaces might have negative response of the activity of the surroundings cells. Terrein was isolated from Penicullium sp. 20135 and found to reduce the effects of LPS-induced inflammation. This study examined the role of Terrein on the biocompatibility of titanium to determine if it can help improve osseointegration. MC-3T3 E1 cells were grown on titanium surfaces. The biocompatibility of Terrein was examined by adding it directly to the culture media at the indicated concentration. The cells on the titanium surface produced excessive ROS and decreased the activity of Cu/Zn SOD and Mn SOD. Moreover, the cells had higher activity towards oxidative stress molecules, such as MAPK, FAK and iNOS expression. In addition, MC-3T3 E1 osteoblast-like cells promoted osteoclast differentiation but reduced osteoblast differentiation and mineralization on the titanium surface. Interestingly, the cells given the Terrein treatment showed higher resistance towards oxidative stress through the up-regulation of ERK1/2 and FAK activity but the down-regulation of SAPK/JNK and iNOS activity. Moreover, Terrein promoted osteoblast differentiation and bone mineralization to elevate the activity of ALP, SPARC and down-regulate RANKL expression after blocking NF-κB translocation from the cytosol to the nucleus. In conclusion, the presence of Terrein on titanium surfaces increases osteoblast cell growth without inflammation. Moreover, Terrein, as a putative antioxidant agent, may enhance osseointegration by decreasing the level of ROS and having a potentially synergistic effect on osteoblast differentiation.

show abstract

Comparative study of antioxidant enzymes in tissues surrounding implant in rabbits

Cited by 40 publications

References 22 publications

Response of human endothelial cells to oxidative stress on Ti6Al4V alloy

Response of human endothelial cells to oxidative stress on Ti6Al4V alloy

Reduced inflammatory responses to poly(lactic-co-glycolic acid) by the incorporation of hydroxybenzyl alcohol releasing polyoxalate

Enhancement of osteoblast biocompatibility on titanium surface with Terrein treatment

Contact Info

Product

Resources

About