Comparative Study of Bacterial Isolates in Ovine Mandibular Osteomyelitis and Oral Microbiota of Healthy Sheep

Ruíz, Héctor; Lacasta, Delia; Bueso, J.P.; Ferrer, Luís Miguel; González, J.M.; Villanueva‐Saz, Sergio; Ramos, Juan José

doi:10.3390/app11093925

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…Of this predominantly strict anaerobic microbiota, there are several opportunistic bacteria with invasive capacity, including aerobic or facultative microorganisms detected when using only conventional-bacterial culture (Benito-Peña et al 2010, Amorim et al 2011, Rasooli et al 2018. Indeed, the richness and diversity of the anaerobic microbiota associated with mandibular infections are underestimated when this diagnostic method is used (Arcaute et al 2021) since some groups of microorganisms, such as spirochetes, cannot be cultivated (Arweiler & Netuschil 2016).…”

Section: Introductionmentioning

confidence: 99%

Fusobacterium necrophorum predominates in the microbiota of mandibular dental abscess in Blastocerus dichotomus

Borsanelli,

Athayde,

Saraiva

et al. 2024

Pesq. Vet. Bras.

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Dental abscess in ruminants is an acute polymicrobial infection, usually resulting from periodontal disease or endodontic infection, with consequences for animal health and welfare. The present study aimed to describe the bacterial microbiota of dental abscesses in Blastocerus dichotomus. Biological material from mandibular dental abscesses, punctured with a sterile syringe and needle during routine veterinary curative procedures or necropsies, was collected from three ex-situ marsh deer. Bacteria were identified using high-throughput sequencing of the 16S ribosomal RNA gene. The three specimens had the presence of facial bulging, and two died because of severe emaciation with a history of progressive weight loss. Bacteroides (38.6%), Fusobacterium (36.65%), and Porphyromonas (7.49%) represented the most abundant genera and Fusobacterium necrophorum (35.69%), Porphyromonas levii (3.12%) and Porphyromonas gulae (1.78%) were among the ten most represented species in the microbiota of mandibular abscess in Blastocerus dichotomus. These molecular findings demonstrate a broader diversity of species in the polymicrobial nature of dental abscesses in B. dichotomus than was previously reported when culture-dependent methods were used in the diagnosis.

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Section: Introductionmentioning

confidence: 99%

Fusobacterium necrophorum predominates in the microbiota of mandibular dental abscess in Blastocerus dichotomus

Borsanelli,

Athayde,

Saraiva

et al. 2024

Pesq. Vet. Bras.

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Oral Microbiome of Bennett's (Notamacropus Rufogriseus) and Yellow-Footed (Petrogale Xanthopus) Rock Wallabies and the Impact of Intraoral Disease

Romano,

Hoyer,

Krumbeck

et al. 2024

Journal of Zoo and Wildlife Medicine

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Atypical Mandibular Osteomyelitis in an Ewe Caused by Coinfection of Pseudomonas aeruginosa and Lactococcus raffinolactis

Garcia Motta,

Martinez,

Pereira Junior

et al. 2022

Acta Scientiae Vet.

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Background: Osteomyelitis is defined as a bone inflammation involving the cortical and medullary regions, usually caused by the local invasion of opportunistic microorganisms. The inflammatory reaction of bone may extend to the periosteum and soft tissues, compromising adjacent structures far from the initially infected foci. Different classifications of transmission routes, gravity levels, and tissues involved in animal and human osteomyelitis are available. In humans, the infection can reach bone tissue by exogenous or hematogenous pathways. This paper reports an atypical case of mandibular pyogranulomatous osteomyelitis in an ewe caused by concomitant Pseudomonas aeruginosa and Lactococcus raffinolactis infection. Case: The animal presented a 1-month history of progressive mandibular enlargement refractory to conventional therapy. In a physical examination, an increased volume located in the ventrolateral region of the right ramus of the mandible was observed. Fine-needle aspiration of the lesion enabled isolation in bacteriological culture of Pseudomonas aeruginosa and Lactococcus raffinolactis using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Besides support care procedures and antimicrobial treatment approaches for the sheep based on in vitro tests, the animal died due to the severity of the clinical signs and the progressive worsening of the general health status. The radiographic image examination of the mandibular region revealed a severe and infiltrative periodontal reaction, with a predominance of a great number of neutrophils and macrophages, necrotic areas, and bone destruction, characterized histologically as a pyogranulomatous rection. At post mortem examination, a large pyogranuloma was observed in the entire horizontal branch of the mandible as well, showing a dark yellowish content of coarse consistency, caseous appearance, and bone fragmentation. Discussion: Ovine mandibular osteomyelitis is a well-established bone inflammation involving the cortical and medullary regions, characterized clinically by local enlargement, asymmetry, pain sensitivity, edema, hyperthermia, infiltrate caseous or suppurative material, and bone rarefaction. In the current report, 1-month history of progressive enlargement of the mandibular region, prostration, and weight loss in an ewe were referred. Where clinical and epidemiological features, bacteriological, cytological, histological, and mass spectrometry diagnostic approaches were assessed to diagnostic. Most reports involving the etiology of ovine mandibular osteomyelitis have been diagnosed based on classical phenotypic tests. Here, the concomitant identification of P. aeruginosa and L. raffinolactis infection was possible using mass spectrometry (MALDI-TOF), highlighting the importance of molecular methods in the diagnosis of animal diseases. In addition, the differentiation between Lactococcus and Enterococcus species is difficult, which could underestimate the diagnosis of Lactococcus species as a primary pathogen from animal diseases. We report, for the first time, a fatal case of mandibular pyogranulomatous osteomyelitis in a sheep caused by Pseudomonas aeruginosa and Lactococcus raffinolactis coinfection. Keywords: sheep, ovine osteomyelitis, Lactococcus sp., Pseudomonas sp., MALDI-TOF MS.

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Comparative Study of Bacterial Isolates in Ovine Mandibular Osteomyelitis and Oral Microbiota of Healthy Sheep

Cited by 3 publications

References 14 publications

Fusobacterium necrophorum predominates in the microbiota of mandibular dental abscess in Blastocerus dichotomus

Fusobacterium necrophorum predominates in the microbiota of mandibular dental abscess in Blastocerus dichotomus

Oral Microbiome of Bennett's (Notamacropus Rufogriseus) and Yellow-Footed (Petrogale Xanthopus) Rock Wallabies and the Impact of Intraoral Disease

Atypical Mandibular Osteomyelitis in an Ewe Caused by Coinfection of Pseudomonas aeruginosa and Lactococcus raffinolactis

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