Comparative Study of Bone Marrow and Blood B Cells in Infantile and Acquired Agammaglobulinemia

Abdou, Nabih I.; Casella, S. R.; Abdou, Nancy L.; Abrahamsohn, Ises A.

doi:10.1172/jci107407

Cited by 26 publications

(3 citation statements)

References 22 publications

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“…Previous studies have shown that contrary to the PB in which there is a greater proportion of IgG-bearing lymphocytes than IgM-bearing lymphocytes, the reverse is true in BM, i.e., there is a significantly greater proportion of IgM-bearing lymphocytes (20,37). This same pattern was noted in the present studies.…”

Section: Human Bone Marrow Lymphocytessupporting

confidence: 83%

“…The use of linear sucrose density gradients to obtain highly purified BM lymphoid cell suspensions (22) in animal studies has allowed for greatly improved investigations of BM lymphocyte populations. This technique has recently been applied to the study of human BM in certain immunodeficiency diseases (15,37) and was used in the present studies to obtain purified mononuclear cell suspensions from BM with relative high cell yields. Because of the conflicting data with regard to the presence of mature T cells within human BM (14)(15)(16)(17)(18)(19)(20), it was considered essential that elements of artifact be controlled as much as possible in the present investigation.…”

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confidence: 99%

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Human bone marrow lymphocytes. I. Distribution of lymphocyte subpopulations in the bone marrow of normal individuals.

Fauci¹

1975

J. Clin. Invest.

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A B S T R A C T This study was undertaken to determine the proportions and in vitro immune capacities of lymphocyte populations in the bone marrows of normal humans. Relatively pure mononuclear cell suspensions were obtained from bone marrow aspirates by linear sucrose gradient centrifugations. Simultaneous peripheral blood and bone marrow specimens from each individual were assayed for lymphocyte surface markers and mitogen responsiveness. Maximal possible contamination of bone marrow aspirates by peripheral blood was determined by performing aspirates on individuals who had received 5'chromium-labeled autologous erythrocytes. Thymus-derived (T) lymphocytes, as determined by the sheep red blood cell (E) rosette assay, comprised 8.6-(±1.6) % of the total bone marrow lymphocyte pool.Bone marrow-derived (B) lymphocytes, as determined by the presence of a complement receptor, made up 15.4-(+1.9) % of the lymphocyte pool whereas 74.6(±2.4) % of mononuclear cells lacked easily detectable surface markers. These findings could not be explained by contamination with peripheral blood lymphocytes since contamination was corrected for in the calculations. Lymphocyte-enriched suspensions of bone marrow cells responded to stimulation with phytohemagglutinin, concanavalin A, and particularly pokeweed mitogen. In vitro incubations of bone marrow and peripheral blood lymphocytes with tritiated thymidine followed by determinations of E and erythrocyte antibody complement (EAC) rosettes were performed. Simultaneous rosetteradioautographs demonstrated that the proliferative potential of bone marrow B lymphocytes was greater than peripheral blood B lymphocytes (P < 0.01). On the other hand, the proliferative potential of bone marrow T

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Section: Human Bone Marrow Lymphocytessupporting

confidence: 83%

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confidence: 99%

Human bone marrow lymphocytes. I. Distribution of lymphocyte subpopulations in the bone marrow of normal individuals.

Fauci¹

1975

J. Clin. Invest.

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“…Adverse reactions to injections of y-globuUn preparations have mostly been presumed to be due to the presence of complement-activating IgG aggregates (5, 8,9,13,16,17,21,26). In addition, IgA deficiency and concomitant antibody formation against IgA have been clearly associated with reactions following infusion of blood products (18,22,25,33,(3)(4) or injection of y-globuIin preparations (1,25,28,34). IgA deficienq-and the presence of class-specific anti-IgA seem to accompany the most severe reactions (6, 24, 25, 28, 33, 34), but reactions also occur in patients with low levels of IgA and antibody of allotype specificity (33, 34).…”

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confidence: 99%

IgA in Commercial γ‐Globulin Preparations

Wadsworth

Hanson

1976

Scand J Immunol

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The presence of anti-IgA has been related to adverse reactions to injection or infusion of IgA-containing material. In this study IgA was demonstrated in all of the investigated commercial gamma-globulin preparations. In the material from a few producers it was, however, very low. By immuno-gel filtration it could be shown that the IgA consisted of aggregates as well as a 7S component and fragments. By immunoelectrophoresis-immunofluorescence IgG-IgA complexes could also be detected. The IgA aggregates could be almost completely degraded by reduction-alkylation. It is implied that such changed IgA in gamma-globulin preparations for injection may increase the risk of immunization against IgA.

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Lymphocyte subpopulations in primary immunodeficiency disorders

Gupta

1982

Indian J Pediatr

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Comparative Study of Bone Marrow and Blood B Cells in Infantile and Acquired Agammaglobulinemia

Cited by 26 publications

References 22 publications

Human bone marrow lymphocytes. I. Distribution of lymphocyte subpopulations in the bone marrow of normal individuals.

Human bone marrow lymphocytes. I. Distribution of lymphocyte subpopulations in the bone marrow of normal individuals.

IgA in Commercial γ‐Globulin Preparations

Lymphocyte subpopulations in primary immunodeficiency disorders

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