2000
DOI: 10.1046/j.1365-2605.2000.00241.x
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Comparative study of disomy and diploidy rates in spermatozoa of fertile and infertile men: a donor‐adapted protocol for multi‐colour fluorescence in situ hybridization (FISH)

Abstract: Spermatozoa from seven healthy donors (two of whom had already fathered children) and five infertile patients taking part in the local programme of intracytoplasmic sperm-injection (ICSI) were investigated for the disomy rates of chromosomes 13/21, 18, X and Y as well as for the diploidy rates. Two- and three-colour fluorescence in situ hybridization (FISH) was applied after a donor-adapted decondensation pre-treatment: in a preliminary decondensation series the optimum fluorescence signals were individually d… Show more

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Cited by 14 publications
(16 citation statements)
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“…Our results are in accordance with the data of many other studies that reported significant negative correlations between sperm concentration and the mean frequency of disomy, especially for the sex chromosomes [6,10,15,21,22]. However, other studies did not show a significant correlation between sex disomy rates and sperm concentration [3,20,23,24]. The disparate results of these studies might be related to their investigation of different types of male infertility: in the majority of cases oligozoospermia was associated with one or more abnormal other parameters.…”
Section: Discussionsupporting
confidence: 90%
“…Our results are in accordance with the data of many other studies that reported significant negative correlations between sperm concentration and the mean frequency of disomy, especially for the sex chromosomes [6,10,15,21,22]. However, other studies did not show a significant correlation between sex disomy rates and sperm concentration [3,20,23,24]. The disparate results of these studies might be related to their investigation of different types of male infertility: in the majority of cases oligozoospermia was associated with one or more abnormal other parameters.…”
Section: Discussionsupporting
confidence: 90%
“…The studies performed thus far largely agree that there is an increased proportion of aneuploid sperm in men with compromised semen parameters. This is with a few notable exceptions (Miharu et al, 1994;Guttenbach et al, 1997;Acar et al, 2000;Damri et al, 2000;Martin et al, 2000;Schultz et al, 2000). The reasons for these differences and those between other individual studies are not entirely clear.…”
Section: Resultsmentioning
confidence: 89%
“…Nevertheless, it is clear that with a few exceptions (Miharu et al, 1994;Guttenbach et al, 1997;Acar et al, 2000;Damri et al, 2000;Martin et al, 2000;Schultz et al, 2000) a general agreement in favour of significant increases in sperm disomy has been established for all chromosomes analysed. Moreover, it is difficult, if not impossible, to compare results of the different papers because the analysed population of males is so heterogenous.…”
Section: Technical Issuesmentioning
confidence: 85%
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