Comparative study of endometrial & subendometrial angiogenesis in unexplained infertile versus normal fertile women

Abstract

Background & objectives: Local angiogenesis in endometrium has been shown to be an essential pre-requisite for endometrial receptivity needed for implantation and gestation. Recently, numerous diagnostic gears have been projected to ‘measure’ or ‘estimate’ the endometrial receptivity relying upon angiogenic factors helping throughout implantation. This study evaluated the endometrial and subendometrial blood flow and the local endometrial gland vascular endothelial growth factor (EG-VEGF) expression as markers of local angiogenesis. Methods: The present study was done to give quantitative assessment of endometrial thickness (ET), endometrial blood flow and subendometrial blood flow colour Doppler indices with endometrial vascular zones. Endometrial biopsy was taken and with VEGF stained and scored with immunohistochemistry. Results: The mean ET for fertile women was 9.41 mm, while in unexplained infertile women it was around 7.90 mm. Upon comparison of ET with EG-VEGF which is considered as a gold standard with correlation coefficient, the present study suggested a positive correlation of EG-VEGF with ET, endometrial pulsatility index (PI), and subendometrial PI also the vascular zones were considered as significant. However, a strong negative correlation was seen with subendometrial resistivity index and PI. Interpretation & conclusions: The present study suggests that uterine ultrasound, uterine colour Doppler and EG-VEGF are parameters which can be used as markers of local angiogenesis for endometrial receptivity in the evaluation of women with unexplained infertility.

Study of the Changes of Pulsatility Index (PI) in Uterine Artery in Patients with Recurrent Pregnancy Loss

“…While some research have hypothezised that unexplained infertility may be caused by inadequate uterine perfusion, there are fewer studies that have investigated the correlation between RPL and uterine artery Doppler flowmetry [8] .…”

Оптимизация Лечения Маточного Бесплодия На Основе Использования Богатого Тромбоцитами Фибрина (I-Prf)

“…В последние годы наблюдается растущий интерес к хроническому эндометриту, особенно в связи с его предполагаемой ролью в бесплодии, привычном невынашивании беременности и повторных неудачах ЭКО [9,[33][34][35][36][37][38].…”

“…Описанные в литературе способы воздействия на рецептивность эндометрия разрозненны, имеют низкую доказательную базу. Было показано, что локальный ангиогенез в эндометрии является важной предпосылкой для рецептивности эндометрия, необходимой для беременности [36].…”