Comparative study of five commercial probes for the detection of Epstein-Barr virus
            (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki’s lymphoma

Nonogaki, Suely; Shirata, Neuza Kasumi; Kimura, Lidia Midori; Guerra, Juliana Mariotti; Medeiros, Raphael Salles Scortegagna de; Paes, Roberto Pinto; Kanamura, Cristina Takami; Oliveira, Camila Carla Maria Ximenes; Menezes, Yara

doi:10.5935/1676-2444.20160065

Cited by 3 publications

(4 citation statements)

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“…The current standard of care is reduction in immunosuppression as the first-line treatment, which might lead to cure in non-invasive and polymorphic PTLDs. 16 - 18 For the remaining PTLD subtypes, there was only one clinical trial showing good results after sequential treatment with rituximab followed by chemotherapy. 17 Reduction in immunosuppression was only seen in 50% of the patients.…”

Section: Discussionmentioning

confidence: 99%

Single-center “Argentine” analysis of post-transplant lymphoproliferative disorders: incidence, histopathological characteristics and EBV status

Warley

Jauk

Otero

et al. 2023

Hematology, Transfusion and Cell Therapy

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Section: Discussionmentioning

confidence: 99%

Single-center “Argentine” analysis of post-transplant lymphoproliferative disorders: incidence, histopathological characteristics and EBV status

Warley

Jauk

Otero

et al. 2023

Hematology, Transfusion and Cell Therapy

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“…Various techniques are available to identify EBV infection, including immunohistochemical (IHC) staining, chromosomal in situ hybridization (CISH), and polymerase chain reaction (PCR). CISH is optimal for detecting EBV infection in formalin-fixed paraffin-embedded tissue due to its higher sensitivity and specificity than IHC 7 . Furthermore, EBVencoded RNA (EBER) detection by CISH is considered the gold standard for verifying the presence of EBV in the nuclei of tumor cells, which cannot be determined using PCR because it does not indicate the origin of the infected cell.…”

Section: Introductionmentioning

confidence: 99%

Epstein–Barr virus-encoded RNA expression and its relationship with the clinicopathological parameters of Vietnamese patients with nasopharyngeal carcinoma

Dung,

Thinh,

Hanh

et al. 2023

Biomed. Res. Ther.

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Introduction: Nasopharyngeal carcinoma (NPC) is a common malignancy in Viet Nam, and its pathogenesis is closely associated with Epstein-Barr virus (EBV) infection. However, the relationship between EBV infection and clinicopathological characteristics related to NPC prognosis in Vietnamese patients remains poorly understood. This study aimed to investigate the association between EBV infection and various clinical parameters in Vietnamese patients with NPC. Methods: We collected clinical data from 31 patients with histologically confirmed NPC and evaluated their samples for EBV-encoded RNA (EBER) expression using the chromosomal in situ hybridization (CISH) technique. We examined the relationship between EBER expression and several clinical parameters, including age, sex, lymph node metastasis, tissue invasion and metastasis, clinical stage, and histological NPC type. Results : The patients' ages ranged from 19 to 74 years, with 80.64% aged >40 years and 87.1% male. In addition, 80.65% had lymph node metastasis, and 38.71% had tissue invasion and distant metastasis. Most patients (67.74%) were diagnosed at a late stage (III or IV), with the most common histological type being type III (48.39%), followed by type I (29.03%) and II (22.58%). EBER expression was observed in 48.39% of the patients and was significantly associated with younger age (<40, p = 0.0362) and undifferentiated carcinoma (type III, p = 0.0007). However, EBER expression was not significantly associated with sex, lymph node metastasis, tissue invasion and metastasis, or clinical stage. Conclusions: Our study suggests that EBV infection may contribute to NPC pathogenesis. It also shows significant associations between EBV infection and younger age and undifferentiated carcinoma type. The CISH technique could help screen asymptomatic high-risk individuals, managing and predicting the NPC prognosis. The small sample size and single-center design limit the generalizability of our findings.

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“…For high-quality (mainly molecular) examination, fixation, post-fixation processing, and preparation of sections must be the priority of pre-analysis team members. 10 Molecular tests are used for latent EBV infection diagnosis and include in situ hybridisation (ISH) and PCR. The membrane latency proteins can also be detected by IHC (Figure 1).…”

mentioning

confidence: 99%

“…Thus, as discussed above, other proteins, including nuclear proteins, may be expressed. 10 Another method for EBV detection is through plasma and peripheral blood mononuclear cell (PBMC) analysis. Kanakry et al 11 studied the clinical significance of PBMC detection, using viral quantitative real-time PCR of plasma and PBMC.…”

mentioning

confidence: 99%

Epstein–Barr Virus: A Biological Overview and Clinicopathological Changes of Two Epstein–Barr Virus-Related Lymphoproliferative Disorders in a World Health Organization (WHO) 2017 Report

Oliveira

2018

EMJ

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Epstein–Barr virus (EBV) is one of the most important viral causes for the development of tumours. The global geographical epidemiology of EBV shows prevalence differences between rich and poor countries across the world, and the impact on health suggests EBV should be an important target of research worldwide. This article will discuss the biology of the virus with an emphasis on its latency types, vital to understanding the possibilities of viral detection. The main objective is to discuss two lymphoproliferative diseases that are associated with EBV and appear in the World Health Organization (WHO) 2017 Classification of Tumours of Haematopoietic and Lymphoid Tissues: EBV-positive diffuse large B cell lymphoma and EBV mucocutaneous ulcer. The name of the former was changed to support the better understanding of infection pathology, while the second was recently described and made its debut in the WHO classification. Pathologists must have knowledge on these diseases and how to investigate them, and oncologists and clinical doctors must be informed on the guidelines.

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Comparative study of five commercial probes for the detection of Epstein-Barr virus (EBV) by in situ hybridization in cases of nodular sclerosis Hodgki’s lymphoma

Cited by 3 publications

References 0 publications

Single-center “Argentine” analysis of post-transplant lymphoproliferative disorders: incidence, histopathological characteristics and EBV status

Single-center “Argentine” analysis of post-transplant lymphoproliferative disorders: incidence, histopathological characteristics and EBV status

Epstein–Barr virus-encoded RNA expression and its relationship with the clinicopathological parameters of Vietnamese patients with nasopharyngeal carcinoma

Epstein–Barr Virus: A Biological Overview and Clinicopathological Changes of Two Epstein–Barr Virus-Related Lymphoproliferative Disorders in a World Health Organization (WHO) 2017 Report

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