A flow-through optosensor for warfarin is described. The sensor is developed in conjunction with flow analysis systems and uses a commercial bound beta-cyclodextrin material as the sensing phase. A strong fluorescence signal was observed as a result of the formation of an inclusion complex between warfarin and beta-cyclodextrin. The analytical performance characteristics of the proposed sensor for analysis of low levels of warfarin were as follows: the detection limits for continuous and flow injection analysis systems were 2 and 19 ppb, respectively; the observed relative standard deviations at 0. 5 ppm warfarin level were less than 2.3%. A study of the interference of other naphthalenic toxic substances was carried out. The continuous flow method was satisfactorily applied to the determination of the rodenticide in natural waters.