Comparative Study of Histopathological Changes Between Nano-Boron and Boron in Small Intestine of Layer Chickens

Abstract

The aim of this study was to compare the effect of boron and nanoboron" on histomorphology of the small intestine in layer chickens. 210 day-old chicks were used and were randomly assigned to seven equal groups. The experiment, groups were exposed to both normal boron and nano-boron as follows:G1 received 15 mg/L of boron in their drinking water, G2 got 25 mg/L, G3 got 45 mg/L, while groups nano boron as following G4 got 15 mg/L, G5 got 25 mg/L, G6 got 45 mg/L of nano-boron, and G7 was not given any treatment. By using a light microscope, the histomorphology result of intestine was examine to determine the villi's height, thickness, crypt depth, and muscle thickness. The measurements of villi height, crypt depth, and muscle thickness showed a significant difference (P≤0.05) between all treated groups (nanoboron at G5,G6 than normal boron) in comparison with control group, with the highest mean in G6(NB) in all measures of intestine. The findings of this study concluded that the adding of "boron and nanoboron" to consumption water improved the structure of intestine, and this was particularly evident at high nanoboron concentrations.

Proteomic Analysis of Rat Duodenum Reveals the Modulatory Effect of Boron Supplementation on Immune Activity

“…This small but crucial segment is essential for digestion, nutrient assimilation, and maintaining a healthy immune response in the body [22]. The study conducted by Abtisam et al (2023) found that the addition of boron (15, 25, and 45 mg/L) and nanoboron (25 and 45 mg/L) to chickens' drinking water can enhance the structure of the small intestine, especially at high nanoboron concentrations [23]. Supplementation of boron in drinking water at 40 and 80 mg/L levels could enhance the structure and function of the duodenum in rats, while higher doses of 320-640 mg/L could have a significant inhibitory effect, according to Liu et al's (2021) study investigating the effects of different doses of boron on the microstructure of the duodenum in rats [24].…”