Comparative study of hyaluronic derivatives: rheological behaviour, mechanical and chemical degradation

Prieto, J. E.; Pulido, Mivis M.; Zapico, J.; Molina, A.; Gimeno, Mercedes; Coronel, Pilar; Alvarez, Ana I.

doi:10.1016/j.ijbiomac.2004.12.003

Cited by 36 publications

(31 citation statements)

References 31 publications

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“…Each author certifies that his or her institution approved the human protocol for this investigation, that all investigations were conducted in conformity with ethical principles of research, and that informed consent for participation in the study was obtained. high-weight molecules contribute to form a high-viscosity solution, which serves as a lubricant and shock absorber [2,25], among other functions. Viscosupplementation reportedly relieves pain [13] but it also is considered a disease-modifying OA drug [14,29] with benefits that have been observed in a period of 6 months to 2 years [22].…”

Section: Introductionmentioning

confidence: 99%

Adding Triamcinolone Improves Viscosupplementation: A Randomized Clinical Trial

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Pailo

et al. 2013

Clinical Orthopaedics &Amp; Related Research

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Section: Introductionmentioning

confidence: 99%

Adding Triamcinolone Improves Viscosupplementation: A Randomized Clinical Trial

Campos

Rezende

Pailo

et al. 2013

Clinical Orthopaedics &Amp; Related Research

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“…For relatively small HA derivatives or fragments, HPLC [8][9][10][11][12] or HPLC-MS [13,14] have been employed. For large underivatized HA, size-exclusion chromatography (SEC) [15][16][17][18][19][20][21][22], SEC combined with viscometry [23][24][25], SEC combined with light scattering (LS) detection (SEC-LS) [23][24][25][26][27][28][29][30][31][32], SEC combined with MALDI-MS (SEC-MALDI-MS) [33][34][35] have been used.…”

Section: Introductionmentioning

confidence: 99%

Extraction of hyaluronic acid (HA) from rooster comb and characterization using flow field‐flow fractionation (FlFFF) coupled with multiangle light scattering (MALS)

Kang

Kim²,

Heo³

et al. 2010

J of Separation Science

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Hyaluronic acid (HA) was extracted in a relatively large scale from rooster comb using a method similar to that reported previously. The extraction method was modified to simplify and to reduce time and cost in order to accommodate a large-scale extraction. Five hundred grams of frozen rooster combs yielded about 500 mg of dried HA. Extracted HA was characterized using asymmetrical flow field-flow fractionation (AsFlFFF) coupled online to a multiangle light scattering detector and a refractive index detector to determine the molecular size, molecular weight (MW) distribution, and molecular conformation of HA. For characterization of HA, AsFlFFF was operated by a simplified two-step procedure, instead of the conventional three-step procedure, where the first two steps (sample loading and focusing) were combined into one to avoid the adsorption of viscous HA onto the channel membrane. The simplified two-step AsFlFFF yielded reasonably good separations of HA molecules based on their MWs. The weight average MW (M(w) ) and the average root-mean-square (RMS) radius of HA extracted from rooster comb were 1.20×10(6) and 94.7 nm, respectively. When the sample solution was filtered through a 0.45 μm disposable syringe filter, they were reduced down to 3.8×10(5) and 50.1 nm, respectively.

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“…Additionally, the operation of BMM has certain risks since concentrated sulphuric acid is used to treat samples and pretreatment is quite complex (Bitter & Muir, 1962). Although other biochemical methods such as specific hyaluronidase digestion and HPLC methods have certain advantages on accuracy and precision, they are rarely applied to determine large batch fermentation broth samples (Gu & Yan, 2003;Prieto et al, 2005).…”

Section: Introductionmentioning

confidence: 99%