Comparative study of inhibition mechanisms of structurally different flavonoid compounds on α-glucosidase and synergistic effect with acarbose

Yang, Jichen; Wang, Xiaoli; Zhang, Chuanying; Ma, Liang; Tao, Wei; Zhao, Yajing; Peng, Xin

doi:10.1016/j.foodchem.2021.129056

Cited by 125 publications

(86 citation statements)

References 37 publications

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“…A previous study also reported that flavonoids with different structures displayed great differences in the α-glucosidase inhibition [19]. Moreover, dietary phenolic compounds could cooperate with acarbose to inhibit α-glucosidase activity [20]. In the current study, the IC 50 value of acarbose was 0.56 ± 0.08 μg/ mL.…”

Section: α-Glucosidase Inhibition Of Myricetrin Andsupporting

confidence: 52%

Inhibitory Effects of Myricetrin and Dihydromyricetin toward α-Glucosidase and Pancreatic Lipase with Molecular Docking Analyses and Their Interaction

Liu

et al. 2021

Journal of Food Quality

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The aim of the current study was to evaluate the interaction effects of myricetrin and dihydromyricetin in inhibiting α-glucosidase and pancreatic lipase at different combination ratios and concentrations and to illuminate the underlying mechanisms of their inhibitions by molecular docking analyses. Results showed that both phenolic compounds possessed good inhibitory effects toward two enzymes in a dose-dependent manner. Myricetrin demonstrated a stronger inhibition against α-glucosidase (IC50, 41.14 ± 2.52 and more than 200 μg/mL, respectively), while dihydromyricetin had a better pancreatic lipase inhibition (IC50, 244.96 ± 4.24 and 373.26 ± 21.36 μg/mL, respectively). Different interaction types were observed when myricetrin and dihydromyricetin inhibited α-glucosidase and pancreatic lipase in combination, which were closely related to the combination ratio and concentration. For α-glucosidase inhibition, synergistic effects were observed at relative low concentrations when the combination ratio of myricetrin to dihydromyricetin was set as 1 : 2, while strong synergistic effects existed at relative high concentrations for pancreatic lipase inhibition. In other combination ratios (1 : 1 or 2 : 1), additive and/or antagonistic effects occurred. Molecular docking analyses showed that myricetrin formed nine hydrogen bonds with α-glucosidase, while only three hydrogen bonds were formed between dihydromyricetin and α-glucosidase. However, these two phenolic compounds had similar hydrogen bonds and hydrophobic interactions with pancreatic lipase. The present study suggested that myricetrin and dihydromyricetin or food materials rich in these two phenolic compounds could be exploited as α-glucosidase and/or pancreatic lipase inhibitors to deal with health problems caused by excessive energy intake, and the combination ratio and concentration of these two phenolic compounds should be considered when producing new functional foods.

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Section: α-Glucosidase Inhibition Of Myricetrin Andsupporting

confidence: 52%

Inhibitory Effects of Myricetrin and Dihydromyricetin toward α-Glucosidase and Pancreatic Lipase with Molecular Docking Analyses and Their Interaction

Liu

et al. 2021

Journal of Food Quality

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“…Also, the synergistic effects from the combination of each flavonoid with acarbose at different concentrations were observed. It was perceived that the best synergistic effect was related to the combined apigenin-acarbose which acted as a noncompetitive inhibitor [ 166 ].…”

Section: Discussionmentioning

confidence: 99%

A Review on Antidiabetic Activity of Centaurea spp.: A New Approach for Developing Herbal Remedies

Fattaheian-Dehkordi

Hojjatifard

Saeedi

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Objective. Diabetes mellitus (DM) is a long-life metabolic disorder, characterized by high blood glucose levels. The hyperglycemic condition generally leads to irreversible nerve injury and vascular damage. Among different types of diabetes, type 2 is more common and has spread all over the world. Although various therapeutic approaches have been developed to control type 2 DM, regulating blood glucose levels has still remained a controversial challenge for patients. Also, most prescription drugs cause different side effects, such as gastrointestinal disorders. Thus, developing novel and efficient antidiabetic agents possessing fewer adverse effects is in high demand. Method. The literature was comprehensively surveyed via search engines such as Google Scholar, PubMed, and Scopus using appropriate keywords. Results. Medicinal plants, both extracts and isolated active components, have played a significant role in controlling the blood glucose levels. Good-to-excellent results documented in the literature have made them a precious origin for developing and designing drugs and supplements against DM. Centaurea spp. have been traditionally used for controlling high blood glucose levels. Also, the antidiabetic properties of different species of Centaurea have been confirmed in recent studies through in vitro assays as well as in vivo experiments. Conclusion. Potent results encouraged us to review their efficacy to open a new horizon for development of herbal antidiabetic agents.

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“…In 298 K, synchronous fluorescence measurement was performed and the wavelength range was 200 to 500 nm 24 . Fixed intervals were Δ λ = 15 nm and Δ λ = 60 nm 25 …”

Section: Methodsmentioning

confidence: 99%

Binding mechanism of lipase with Lentinus edodes mycelia polysaccharide by multi‐spectroscopic methods

Liu

Chen

et al. 2021

J of Molecular Recognition

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It is an effective strategy to avoid obesity by inhibiting the activity of lipase. In this study, the binding mechanism of lipase and Lentinus edodes mycelia polysaccharide (LMP) were explored with multi‐spectral methods, for example, three‐dimensional (3D) fluorescence, Fourier‐transformed infrared (FT‐IR), and Raman spectra. At 290 K, the binding constant was 2.44 × 105 L/mol, there was only one binding site between LMP and lipase. Static quenching was the quenching mechanism. The major forces were hydrogen bonding and van der Waals force. The binding of LMP to lipase impacted the microenvironment around tyrosine and tryptophan residues. The polarity around these residues was decreased and hydrophobicity was enhanced. This study not only revealed the binding mechanism of LMP on lipase but also provided scientific evidence for expanding the application of LMP in functional food industries.

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Comparative study of inhibition mechanisms of structurally different flavonoid compounds on α-glucosidase and synergistic effect with acarbose

Cited by 125 publications

References 37 publications

Inhibitory Effects of Myricetrin and Dihydromyricetin toward α-Glucosidase and Pancreatic Lipase with Molecular Docking Analyses and Their Interaction

Inhibitory Effects of Myricetrin and Dihydromyricetin toward α-Glucosidase and Pancreatic Lipase with Molecular Docking Analyses and Their Interaction

A Review on Antidiabetic Activity of Centaurea spp.: A New Approach for Developing Herbal Remedies

Binding mechanism of lipase with Lentinus edodes mycelia polysaccharide by multi‐spectroscopic methods

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