Comparative study of lignins produced by the tea-plant and by tea-plant derived callus tissues

Zaprometov, M. N.; Загоскина, Н. В.; Елкин, В. В.

doi:10.1016/s0031-9422(00)95158-6

Cited by 7 publications

(4 citation statements)

References 3 publications

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“…This is consistent with the results of lignin analysis of cultured cells of gymnosperm (Brunow et al 1990(Brunow et al , 1993Fukuda et al 1988; Lange et al 1995) and angiosperm trees (Christiernin et al 2005;Higuchi and Barnoud 1966;Higuchi et al 1960;Wolter et al 1974;Zaprometov et al 1993). Normal developmental or constitutive lignins of gymnosperms and angiosperms are composed of guaiacyl and guaiacyl/ syringyl aromatic rings, respectively, while grass lignins contain significant amounts of p-hydroxyphenyl, as well as guaiacyl and syringyl units (Sarkanen and Hergert 1971).…”

Section: Resultssupporting

confidence: 83%

“…Higuchi et al (1966), Wolter et al (1974), and Christiernin et al (2005) reported similar results for a number of cultured cell lines of angiosperm tree species including Populus tremuloides and Populus tremulaϫtremuloides. In addition, the lignin in Camellia sinensis cells cultured under heterotrophic conditions contained high levels of p-hydroxyphenyl lignin (Zaprometov et al 1993). Similarly, lignins from suspension cultures of the gymnosperm trees, Pinus taeda and Picea abies had lower OCH 3 contents than their corresponding woody tissues (Brunow et al 1990;Fukuda et al 1988).…”

Section: Resultsmentioning

confidence: 97%

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Occurrence of guaiacyl/p-hydroxyphenyl lignin in Arabidopsis thaliana T87 cells

Yamamura

Wada

Sakakibara

et al. 2011

Plant Biotechnology

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Abstruct Arabidopsis thaliana is the most widely used model plant in the area of plant biosciences, and the cell line T87 is one of the most commonly used lines in Arabidopsis cell cultures. The characteristics of lignins in cultured cells often differ from those of lignins in intact plants. To date, nothing is known about the lignins of T87 cells. In this study, we characterized lignins of T87 cells using six analytical methods; phloroglucinol staining, thioacidolysis, nitrobenzene oxidation, alkaline hydrolysis, and Klason and acetyl bromide methods. These analyses clearly showed that lignins of T87 cells are composed of guaiacyl and p-hydroxyphenyl units, and are quite different from lignin found in the Arabidopsis inflorescence stem, which is composed of guaiacyl and syringyl units. Materials and methods T87 cells and plant materialsT87 suspension-cultured cells were cultivated in 200 ml mJPL3 liquid medium according to Ogawa et al. (2008b) and harvested at 1-21 days after subculturing. The harvested T87 suspensioncultured cells were collected onto filter paper (Advantec Co., Ltd.) in a Buchner funnel. After removal of the medium, the collected cells were washed with 50 ml MilliQ water, dewatered by suction filtration for 30 s, weighed, and then stored in liquid nitrogen until use. In addition, T87 cells were maintained on mJPL3 agar medium and subcultured every 14 days. A. thaliana Columbia plants were cultivated under a 16-h light/8-h dark regime as reported previously (Nakatsubo et al. 2008a(Nakatsubo et al. , 2008b. Sample preparation for lignin analysesHarvested T87 suspension-cultured cells and A. thaliana inflorescence stems (60 days old, 30 cm in height) were individually freeze-dried, powdered with a pestle and mortar, and extracted with 95% methanol at 60°C until the color of the powdered cells changed from green to pale yellow. Then, the powders were further extracted successively with hexane and distilled water and freeze dried. Hereafter, these samples are referred to as extracts-free powders in this paper. Then, the extracts-free powders were dried in an oven at 60°C to constant weight. The lignins in the dried extract-free powders were then analyzed by thioacidolysis, nitrobenzene oxidation, alkaline hydrolysis, and Klason and acetyl bromide methods. Chemicals InstrumentationCells were observed under an Olympus BX51 microscope (Olympus Co., Ltd), and images were obtained using a digital camera (Olympus DP71, Olympus Co., Ltd.) connected to the microscope.GC-MS was performed using a Shimadzu QP-5050A GC-MS system (Shimadzu Co., Ltd.). The GC-MS conditions were as follows: Shimadzu Hicap CBP10-M25-0.25 column (25 mϫ 0.22 mm); carrier gas, helium; injection temperature, 230°C; oven temperature, 40°C at tϭ0 to 2 min, then to 230°C at 40°C min Ϫ1; ionization, electron-impact mode (70 eV). Absorbance was measured with a UV-1600PC UV-visible spectrophotometer (Shimadzu Co., Ltd.). Phloroglucinol-hydrochloric acid stainingThe phloroglucinol-hydrochloric acid reaction was carried out according to the...

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 97%

Occurrence of guaiacyl/p-hydroxyphenyl lignin in Arabidopsis thaliana T87 cells

Yamamura

Wada

Sakakibara

et al. 2011

Plant Biotechnology

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“…In respect to lignin composition, the labile ether fraction represented a very low proportion of in situ lignin and was composed mainly of monomethoxylated monomers. These structural features are in agreement with previous reports of highly condensed guaiacyl type of lignin encountered in vessels (Terashima et al 1993) and in cell suspension cultures (Venverloo 1971; Fukuda 1983; Zaprometov et al 1993). In some transgenic cell lines, the amounts of guaiacyl units were lower than that of the control.…”

Section: Discussionsupporting

confidence: 93%

Overexpression of a heterologoussamgene encoding S‐adenosylmethionine synthetase in flax (Linum usitatissimum) cells: Consequences on methylation of lignin precursors and pectins

Lamblin

Saladin

Dehorter³

et al. 2001

Physiologia Plantarum

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The Arabidopsis thaliana sam1 gene encoding S-adenosylmethionine synthetase (EC 2.5.1.6) was transferred to flax (Linum usitatissimum) cells via Agrobacterium tumefaciens. This enzyme catalyses the conversion of methionine to S-adenosylmethionine (SAM), the major methyl group donor in living cells. The aim of this work was to study the consequences of an increased SAM-synthetase (SAM-S) activity in transgenic cell lines on both the production of mono- and dimethoxylated lignin monomers and the degree of methylesterification of pectins. Hypocotyls were cocultivated with Agrobacterium tumefaciens strain GV3101 (pGV2260) harbouring the pO35SSAM binary vector carrying the sam1 gene under the control of the 35S promoter and the nptII gene for selection of putative transformed cells. Most of the transgenic cell lines exhibited a significant (up to 3.2-fold) increase in SAM-S activity compared to the controls. The results showed that for the cell lines analysed this transformation had no effect on caffeic acid O-methyltransferase (COMT, EC 2.1.1.68) in vitro activity, degree of methoxylation of lignin precursors or lignin deposition, pectin methyltransferase (PMT, EC 2.1.1) in vitro activity, but led to an increase of pectin methylesterification in friable and fast-growing transgenic cell lines.

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“…The cell walls of plants contain cellulose, hemicellulose, and lignin. The content and composition of these polymers vary among the different plant organs and within a specific tissue in the plants ( Zaprometov et al, 1993 ; Campbell and Sederoff, 1996 ; Schuetz et al, 2013 ; Lourenço et al, 2016 ). In addition, lignin variation is affected by environmental conditions and plant growth stages ( Ralph and Hatfield, 1991 ; Buranov and Mazza, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

The Phenylpropanoid Pathway and Lignin in Defense against Ganoderma boninense Colonized Root Tissues in Oil Palm (Elaeis guineensis Jacq.)

et al. 2017

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Basal stem rot, caused by the basidiomycete fungus, Ganoderma boninense, is an economically devastating disease in Malaysia. Our study investigated the changes in lignin content and composition along with activity and expression of the phenylpropanoid pathway enzymes and genes in oil palm root tissues during G. boninense infection. We sampled control (non-inoculated) and infected (inoculated) seedlings at seven time points [1, 2, 3, 4, 8, and 12 weeks post-inoculation (wpi)] in a randomized design. The expression profiles of phenylalanine ammonia lyase (PAL), cinnamyl alcohol dehydrogenase (CAD), and peroxidase (POD) genes were monitored at 1, 2, and 3 wpi using real-time quantitative polymerase chain reaction. Seedlings at 4, 8, and 12 wpi were screened for lignin content, lignin composition, enzyme activities (PAL, CAD, and POD), growth (weight and height), and disease severity (DS). Gene expression analysis demonstrated up-regulation of PAL, CAD, and POD genes in the infected seedlings, relative to the control seedlings at 1, 2, and 3 wpi. At 2 and 3 wpi, CAD showed highest transcript levels compared to PAL and POD. DS increased progressively throughout sampling, with 5, 34, and 69% at 4, 8, and 12 wpi, respectively. Fresh weight and height of the infected seedlings were significantly lower compared to the control seedlings at 8 and 12 wpi. Lignin content of the infected seedlings at 4 wpi was significantly higher than the control seedlings, remained elicited with no change at 8 wpi, and then collapsed with a significant reduction at 12 wpi. The nitrobenzene oxidation products of oil palm root lignin yielded both syringyl and guaiacyl monomers. Accumulation of lignin in the infected seedlings was in parallel to increased syringyl monomers, at 4 and 8 wpi. The activities of PAL and CAD enzymes in the infected seedlings at DS = 5–34% were significantly higher than the control seedlings and thereafter collapsed at DS = 69%.

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Comparative study of lignins produced by the tea-plant and by tea-plant derived callus tissues

Cited by 7 publications

References 3 publications

Occurrence of guaiacyl/p-hydroxyphenyl lignin in Arabidopsis thaliana T87 cells

Occurrence of guaiacyl/p-hydroxyphenyl lignin in Arabidopsis thaliana T87 cells

Overexpression of a heterologoussamgene encoding S‐adenosylmethionine synthetase in flax (Linum usitatissimum) cells: Consequences on methylation of lignin precursors and pectins

The Phenylpropanoid Pathway and Lignin in Defense against Ganoderma boninense Colonized Root Tissues in Oil Palm (Elaeis guineensis Jacq.)

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