Comparative study of &lt;i&gt;in vitro &lt;/i&gt;antioxidant activity of foliar endophytic fungi and leaves extracts of &lt;i&gt;Peganum harmala&lt;/i&gt; of Dayate aiat (Laghouat, Algeria)

Ouzid, Yasmina; Smail-Saadoun, Noria; Houali, Karim

doi:10.4314/jfas.v10i1.10

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…The decrease of the mitotic index following the increase in the concentrations of extracts indicates a mitodepressive effect of the methanolic extract of the plant leaves. A similar study, performed with the crude fungal extracts of endophytic foliar fungi of P. harmala, using ethyl acetate as extraction solvent, revealed comparable results (Ouzid et al, 2019). The plant is known for its richness in alkaloids (harmines, harmalines), phenols, terpenes and saponosides with cytotoxic activity; according to El-ghamery et al 2007), the decrease in the mitotic index is probably due to a modification or alteration in the expression of certain genes, each action occurring separately in the interphase nucleus through the ultimate influence of the structure.…”

Section: Table 1 Mitotic Indexes and Limit Values For Cytotoxicity Un...mentioning

confidence: 61%

Antimitotic and genotoxic effect of methanolic extracts of leaves of Peganum harmala L. on the meristematic cells of Allium cepa L.

Ouzid,

Kaci-Boudiaf,

Zeghouini

et al. 2023

Bioagro

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Medicinal plants are an inexhaustible source of molecules with several biological activities. The main objective of this research was the evaluation of the antimitotic and genotoxic effect by the Allium cepa test of the methanolic extract of the leaves of Peganum harmala, a spontaneous plant from the arid zones of Algeria (Timzerth, Laghouat). Different concentrations (1, 4, 10, and 16 mg∙mL-1) of the extract, as well as two standards (colchicine and quercetin) at 1 mg∙mL-1 concentration were tested. Mitotic index (MI), limit value for cytotoxicity (LVC), phase index (PI), and aberration indexes (AI) were calculated, and different aberrations were identified in treated meristematic onion cells. Statistical analyses by ANOVA and Newman-Keuls test were performed. The results confirmed our previous findings that the methanolic extract has an anti-mitotic effect similar to that of the standards. The increase in extract concentrations was accompanied by a decrease in the various indexes calculated. The extracts blocked mitosis in prophase which was predominantly present, and caused increase in abnormalities, represented by dominant rates of agglutinations, chromosomal fragmentations and binuclear cells. confirmed that the effect was independent of concentrations, though to varying degrees.

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Section: Table 1 Mitotic Indexes and Limit Values For Cytotoxicity Un...mentioning

confidence: 61%

Antimitotic and genotoxic effect of methanolic extracts of leaves of Peganum harmala L. on the meristematic cells of Allium cepa L.

Ouzid,

Kaci-Boudiaf,

Zeghouini

et al. 2023

Bioagro

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“…2010, El- Negerabi et al . 2013, Ouzid et al 2018, Sathiyabama and Parthasarathy 2018) and as epifoliar fungi: Aspergillus , Aureobasidium , Cladosporium , Mucor , Penicillium and Schizophyllum (Kharwar et al . 2010, Salazar-Cerezo et al .…”

Section: Discussionmentioning

confidence: 99%

“…2010), while it is different from that obtained for Pinus resinosa (0.12; Legault et al . 1989), Nothofagus truncata (0.78; Ruscoe 1971) and Peganum harmala (0.62; Ouzid et al . 2018), whose obtained values show a high similarity of the two fungal communities of these plants.…”

Section: Discussionmentioning

confidence: 99%

Diversity of Epiphytic and Endophytic Fungal Communities Associated with Leaves of Ziziphus lotus (L.) Lam. from Algeria

Ghazi-Yaker,

Kraak,

Houbraken

et al. 2023

Polish Journal of Ecology

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In this study, the diversity of endophytic and epiphytic fungi communities was evaluated and compared from healthy, symptomless and green leaves of the medicinal plant Ziziphus lotus (wild jujube) growing in Northern Algeria. Culture-based methods were used to isolate the endophytes and epiphytes. Morphological and molecular analyses were applied for identification of the fungi taxa. Both communities of the phylloplane of Ziziphus lotus differed in size and composition. Out of all 52 species, 19 were identified as epiphytic fungi and 45 as endophytes with 12 species shared by the two communities (Aspergillus costaricaensis, A. tubingensis, Aureobasidium sp., Cladosporium asperlatum, C. halotolerans, C. limoniforme, Penicillium chrysogenum, P. desertorum, P. flavigenum, P. momoii, Stemphylium sp. and Trichothecium crotonigenum). The two communities were found to be moderately similar (Sørensen similarity index = 0.37). Higher diversity values were found in endophytic community compared to epiphytic community: Shannon's diversity index H' (3.29 vs 1.83), Simpson diversity index C' (0.94 vs 0.74), species richness (3.06 vs 0.46) and species evenness (0.86 vs 0.62). Additionally, the correlation matrix was generated for the identification of possible interactions between different fungi.

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“…Certains travaux ont concerné des plantes des plantes spontanées, tels Bensaci et al (2006) [16], qui ont montré la diversité en champignons endophytes au niveau des aiguilles du cèdre de l'Atlas du massif de Bélezma (Aurès), Zerroug (2011) sur les champignons endophytes isolés de Retama raetam (Forsk.) collecté dans la région de Bordj Bou Arreridj [17], ainsi que Ladjal (2012) qui a fait une identification de champignons endophytes au niveau des aiguilles de Pinus halepensis du canton d'El-Haourane de la région de M'sila [18] et Ouzid et al (2018) [19], dont le travail a concerné une étude comparative de l'activité antioxydante de champignons endophytes foliaires et des extraits des feuilles de Peganum harmala de la région de Laghouat.…”

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Interannuals variations in diversity of foliar mycoendophytes of Pistacia atlantica from dayate Aiat (Laghouat, Algeria)

Zareb¹,

Smail-Saadoun²

2023

J. Fundam and Appl Sci.

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Endophytic fungi live inside plant tissues and increase their resistance to abiotic and biotic factors. Our work is concerned with interannual variations of mycoendophyte diversity of leaves of Pistacia atlantica Desf. of dayate Aiat, in the region of Laghouat (Algeria) and this for the spring of 2013 and 2016. The morphological identification revealed the presence of 26 fungal genera. The difference in diversity is very significant between the two years of sampling, according to calculated Shannon Weaver indices. Based on principal component analysis, the distribution of these leaf microorganisms is influenced by the climatic conditions of the study area. Aspergillus and Epicoccum are dominant for 2013. In 2016, Aspergillus and Cladosporium dominate. The persistence of Aspergillus gives it an important role in the fight against the stress suffered by the leaf of Pistacia atlantica.

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Comparative study of <i>in vitro </i>antioxidant activity of foliar endophytic fungi and leaves extracts of <i>Peganum harmala</i> of Dayate aiat (Laghouat, Algeria)

Cited by 4 publications

References 6 publications

Antimitotic and genotoxic effect of methanolic extracts of leaves of Peganum harmala L. on the meristematic cells of Allium cepa L.

Antimitotic and genotoxic effect of methanolic extracts of leaves of Peganum harmala L. on the meristematic cells of Allium cepa L.

Diversity of Epiphytic and Endophytic Fungal Communities Associated with Leaves of Ziziphus lotus (L.) Lam. from Algeria

Interannuals variations in diversity of foliar mycoendophytes of Pistacia atlantica from dayate Aiat (Laghouat, Algeria)

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