Comparative study of mesenchymal stem cells from C57BL/10 and mdx mice

Abstract

Background: Human mesenchymal stem cells (MSCs) have been studied and applied extensively because of their ability to self-renew and differentiate into various cell types. Since most human diseases models are murine, mouse MSCs should have been studied in detail. The mdx mouse -a Duchenne muscular dystrophy model -was produced by introducing a point mutation in the dystrophin gene. To understand the role of dystrophin in MSCs, we compared MSCs from mdx and C57BL/10 mice, focusing particularly on the aspects of light and electron microscopic morphology, immunophenotyping, and differentiation potential.

Cell Growth Characteristics, Differentiation Frequency, and Immunophenotype of Adult Ear Mesenchymal Stem Cells

“…This time course is consistent with the other studies. Mouse bone marrow and lung mesenchymal progenitor cell populations demonstrate a cell cycle length of ~2-3 days [22,23], whereas a complete cell cycle for adipose-derived stromal/stem cells takes ~1.5-2.8 days in humans [2,19] and 3.3 in pigs [20]. Since cell seeding density is also considered as a factor that plays a role in the expansion capacity of MSCs, we determined doubling times of EMSCs in relation to cell plating density.…”

Cell rolling column in purification and differentiation analysis of stem cells

“…In contrast, marker molecules for the MSCs whose undifferentiation tendency is much higher than that of HSCs and EPCs are still unclear, and the expression levels of the marker molecules seem to change as they differentiate. Studies have shown that the CD34 level of MSCs or HSCs is not stable [29,30]. …”

Antibody‐immobilized column for quick cell separation based on cell rolling

“…15,16 For example, the CD34 expression level of hematopoietic stem cells continuously decreases with developmental stage. 17 In myogenic progenitor cells, the expression level of CD34 changes during its differentiation into a myotube.…”