Comparative study of Mg/Al- and Zn/Al-layered double hydroxide-perindopril erbumine nanocomposites for inhibition of angiotensin-converting enzyme

Abstract

Abstract:The intercalation of a drug active, perindopril, into Mg/Al-layered double hydroxide for the formation of a new nanocomposite, PMAE, was accomplished using a simple ion exchange technique. A relatively high loading percentage of perindopril of about 36.5% (w/w) indicates that intercalation of the active took place in the Mg/Al inorganic interlayer. Intercalation was further supported by Fourier transform infrared spectroscopy, and thermal analysis shows markedly enhanced thermal stability of the active. The release of perindopril from the nanocomposite occurred in a controlled manner governed by pseudo-second order kinetics. MTT assay showed no cytotoxicity effects from either Mg/Al-layered double hydroxide or its nanocomposite, PMAE. Mg/Al-layered double hydroxide showed angiotensin-converting enzyme inhibitory activity, with 5.6% inhibition after 90 minutes of incubation. On incubation of angiotensin-converting enzyme with 0.5 µg/mL of the PMAE nanocomposite, inhibition of the enzyme increased from 56.6% to 70.6% at 30 and 90 minutes, respectively. These results are comparable with data reported in the literature for Zn/Al-perindopril.

Preparation and controlled-release studies of a protocatechuic acid-magnesium/aluminum-layered double hydroxide nanocomposite

“…14 Owing to the efficient drug-delivery feature of LDH nanocarriers, many pharmaceutically active compounds have been successfully intercalated into the interlayer gallery of LDH. Among them are the cardiovascular drugs fluvastatin and pravastatin; 15 anti-inflammatory drugs such as diclofenac 16 and fenbufen; 17 antihypertensive drugs like perindopril erbumine; 18,19 the antihistamine drug cetirizine hydrochloric acid; 20 and anticancer drugs such as cordycepin, which was intercalated into the gallery of magnesium/ aluminum (Mg/Al)-LDH. It was observed that the resulting nanohybrid had greater stability and a greater suppression effect on U937 cancer cell growth than free cordecypin 3 and methotrexate (MTX).…”

Development of a biocompatible nanodelivery system for tuberculosis drugs based on isoniazid-Mg/Al layered double hydroxide

“…16,37 spatial orientation of INh into the interlayer gallery of Mg/al lDh Figure 2A shows the values for the long and short axes and thickness of INH, which were 10.5 Å , 7.2 Å, and 3.1 Å, respectively, as reported previously. 30 The average basal spacing for INH-Mg/Al LDH prepared by the ion exchange method (nanocomposite A) was found to be 12.00 Å. By subtracting the LDH layer thickness of 4.8 Å from the average basal spacing of 12.00 Å, we obtained 7.2 Å, which was exactly equal to the short (horizontal) axis of INH.…”

“…30 In brief, Mg(NO 3 ) 2 hexhydrated and Al(NO 3 ) 3 non-hydrated at a 1:2 ratio were dissolved in 250 mL of deionized water and stirred for 15 minutes while Mg/Al LDH was formed by raising the pH to 10 by the dropwise addition of a 2 mol/L sodium hydroxide aqueous solution. Next, 50 mL of a 1 mol/L solution of isoniazid was added to freshly prepared Mg/Al LDH and the sample was stirred for 2 hours.…”

“…24,30 In short, the Mg(NO 3 ) 2 hexhydrated and Al(NO 3 ) 3 nonhydrated at a 1:2 ratio were dissolved in 250 mL of deionized water and stirred for 15 minutes. Next, 50 mL of a 1 mol/L aqueous solution of isoniazid was added.…”

Systemic toxicity induced by aggregated layered double hydroxide nanoparticles

“…5,13,14 Several researchers have conjugated a variety of drugs (including the anti-tuberculosis agent paraaminosalicylic acid; 15 glucocorticoid drug betamethasone sodium phosphate; 16 antihypertensive prodrug perindopril erbumine; 17 anticancer moieties such as chlorogenic acid, 18 10-hydroxycamptothecin, 19 and protocatechuic acid; 20 photosensitizer indole-3-acetic acid; 21 and the poor water-soluble drug ibuprofen, 22 as well as nucleic acids 23 and enzymes 24 ) into the interspaces of LDH nanoparticles and successfully delivered them into cells.…”