Comparative study of microbial, proximate and heavy metal compositions of some gastropods, bivalve and crustacean seafood

Abstract

Background: Seafood are known to be nutritionally rich, however, water bodies from where these foods are harvested are often burdened with wastes from industries, household and run-offs. Thus seafood can harbor pathogenic microorganisms and heavy metals which can pose a serious health hazard to consumers. Materials and Methods: In this study, 5kg each of Littorina littorea, Achatina fulica,Tympanotonos fuscatus, Dorsanum miran, Egeria radiate and Penaeus notialis were investigated for microbial, proximate and heavy metal qualities using standard microbiological and chemical methods of analysis. Results: The gastropod mollusks Tympanotonos fuscatus, Littorina littorea and Achatina fulica had significantly (p≤0.05) higher microbial loads compared to other samples. All the samples had significantly (p≤0.05) higher coliform counts compared to standard specifications. The microbial isolates from the samples included species of Bacillus, Klebsiellae, Citrobacter, Providencia, Pseudomonas, Staphylococcus aureus, Escherichia coli and Aspergillus niger, Aspergillus flavus, yeasts, Alternaria, Absidia, Rhizopus, and Mucor. Antibiogram of the isolates revealed multiple resistances with 79.55% and 66.67% resistance to antibacterial and antifungal agents respectively. The seafood was rich in protein (8.0±0.03 to 46.0±0.03%) and fat (0.6±0.01 to 8.8±1.00%) but low in carbohydrate (1.18±0.84 to 3.81±0.86%).The heavy metal compositions of the seafood were generally low except for manganese 6.36±0.03ppm in Dorsanum miran and nickel 0.81±0.50 ppm in Penaeus notialis. Conclusion: The microbial loads of the seafood are significantly (p≤0.05) higher in gastropods and more than acceptable standard, effective protection of water bodies for food cultivation and constant monitoring of seafood is necessary. Adequate processing and employing good manufacturing practices can reduce the microbial loads to an acceptable level and prevent food borne hazards that could be associated with seafood consumption. Keywords: Seafood; Heavy metals; Microbial profile; proximate compositions ÉTUDE COMPARATIVE DE LA PROLIFÉRATION MICROBIENNE, PROCHAINE ET HEAVY METAL COMPOSITIONS DE CERTAINS GASTÉROPODES, MOLLUSQUES ET CRUSTACÉS LES FRUITS DE MER

Effects of Seasonal Variations on the Heavy Metal and Proximate Composition of Prawn Samples from Two Locations in Bayelsa State, Nigeria

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Bacterial Isolates from Bivalve Clams (Galatea paradoxa, Born 1778): Occurence, Multi-drug Resistance, Location of Antibiotic Resistance Marker and Plasmid Profiles

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“…Thereafter, the discrete colonies were sub-cultured onto plates of freshly prepared nutrient agar and aerobically incubated at 37°C for 24 hr. The pure cultures of isolates were streaked onto nutrient agar slants, incubated at 37°C for 24…”

Reducing Escherichia Coli Contamination on Dringking Water Stations in Pasuruan, East Java, Indonesia.

“…In order to protect human health, appropriate control measures to eliminate human pathogen should be developed. Oranusi et al [30] found the microbial loads of the seafood's were more than acceptable standard (significantly higher in gastropods, p≤0.05). Water body protection and seafood monitoring were necessary.…”