Comparative study of new shell-type, sub-2μm fully porous and monolith stationary phases, focusing on mass-transfer resistance

Oláh, Erzsébet; Fekete, Szabolcs; Fekete, Jenő; Ganzler, Katalin

doi:10.1016/j.chroma.2010.03.052

Cited by 158 publications

(82 citation statements)

References 45 publications

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“…In the recent development of particle technology for liquid chromatography, the use of core-shell particles has received considerable attention [10][11][12][13][14][15][16]. The particle size is also a beneficial factor as it improves the rate of mass transfer and reduces the eddy dispersion effect, resulting in smaller plate height and higher optimum linear velocity as the particle size is reduced [17,18].…”

Section: Resultsmentioning

confidence: 99%

Enhancing the Quality of Separation in One-Dimensional Peptide Mapping Using Mathematical Transformation

Berky

Fekete

2012

Chromatographia

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In this study, some practical examples are presented that show the quality of separations using very efficient columns packed with the latest generation of core shell sub-3 lm and fully porous sub-2 lm particles in onedimensional peptide separations. This paper shows an approach for the analysis of proteins, such as high-resolution separations, and a data transformation process to improve peak recognition and analysis. Applying power functions on raw chromatographic data can be a neat tool in the field of biosimilar analysis, especially in comparability studies regarding the quality (primary structure) of proteins. Based on the results presented here, it can be stated that the use of power functions is beneficial for the comparison of chromatograms when peak areas are considered but has no effect when using peak heights. In this study, the new Acquity CSH columns (C18 and phenyl-hexyl) and the core-shell type wide pore Ascentis Express Peptide ES C18 material were applied with great success in peptide mapping. Finally, using phenyl-hexyl stationary phase in peptide separation seems to be a good alternative to the generally applied C18 or C4 phases.

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Section: Resultsmentioning

confidence: 99%

Enhancing the Quality of Separation in One-Dimensional Peptide Mapping Using Mathematical Transformation

Berky

Fekete

2012

Chromatographia

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“…Recently, Gritti et al [12] have compared Kinetex C18 column and the sub-2 lm particles Acquity BEH C18 column for analysis of large molecule mixtures and have obtained similar peak capacities for both columns. Even though, several publications have investigated Kinetex C18 performance [13][14][15], there is a lack of studies comparing this column with conventional sub-2 lm particles columns for specific applications in gradient mode.…”

Section: Introductionmentioning

confidence: 98%

Comparison of LC Columns Packed with 2.6 μm Core-Shell and Sub-2 μm Porous Particles for Gradient Separation of Antibiotics

et al. 2011

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The recently introduced Kinetex C18 column packed with core-shell 2.6 lm particles is declared to provide similar efficiency and short analysis as Acquity BEH C18 column with 1.7 lm porous particles. Unlike Acquity BEH C18 column, Kinetex C18 column exhibited lower column backpressure making this column compatible to conventional LC systems. The performance of Kinetex C18 column (2.1 9 50 mm) and Acquity BEH C18 column (2.1 9 50 mm) for gradient separation of tetracyclines under acidic conditions (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) and macrolides under alkaline conditions (tylosin, clarithromycin, roxithromycin, and carbomycin) was studied. The columns were compared by evaluation of their experimental peak capacity and its dependence on linear velocity and gradient slope. The maximal experimental peak capacities for analysis of tetracyclines were 51.8 (Acquity BEH C18 column) and 48.4 (Kinetex C18 column). This indicated that Kinetex C18 was a suitable alternative to Acquity BEH C18 column for the analysis of tetracyclines under acidic conditions. On the contrary, the maximal experimental peak capacities for analysis of macrolides on Acquity BEH C18 column was higher (46.7) than that on Kinetex C18 column (36.9). Moreover, application of Kinetex C18 column for the analysis of macrolides under alkaline conditions was limited with respect to its decreasing performance with growing number of injections on the column.

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“…The three column technologies were already compared [14,15]. Trends are not absolutely toward any of the tree approaches.…”

Section: Introductionmentioning

confidence: 99%

Monolithic Silica for Fast HPLC: Current Success and Promising Future

Deeb

2011

Chromatographia

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As an approach for fast HPLC, monolithic silica has proven to be highly effective. It is especially successful for routinely obtaining fast isocratic HPLC analyses of small drug molecules. The low cost of monolithic compared with other approaches, such as UPLC, makes it more convenient for everyday application. It is also the more developed and widely applied technique compared with superficially porous particles. It offers the possibility for gaining high plate numbers through column coupling, but not at the expense of run time if a proper flow program is subsequently applied. Good precision and batch reproducibility are now achieved with commercially available monolithic silica columns. The application of monolithic silica columns is already well developed in various fields. It invades the field of bio-analysis and proteomics. Hundreds of analytical methods have already been successfully transferred to or developed on monolithic silica columns. An updated strategy based on Snyder's method for rapid method development using monolithic column has been provided. However, more is still to be expected from monolithic silica in term of chemistry variation, application, and instrument compatibility. The future of monolithic silica is promising when considering the high demand for fast chromatographic analysis.

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Comparative study of new shell-type, sub-2μm fully porous and monolith stationary phases, focusing on mass-transfer resistance

Cited by 158 publications

References 45 publications

Enhancing the Quality of Separation in One-Dimensional Peptide Mapping Using Mathematical Transformation

Enhancing the Quality of Separation in One-Dimensional Peptide Mapping Using Mathematical Transformation

Comparison of LC Columns Packed with 2.6 μm Core-Shell and Sub-2 μm Porous Particles for Gradient Separation of Antibiotics

Monolithic Silica for Fast HPLC: Current Success and Promising Future

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