Comparative study of permeation effects between vibrating microneedle and low-frequency sonophoresis systems

Abstract

Background: Microneedle transdermal administration and low-frequency ultrasound represent two important physical penetration-promoting methods for enhancing drug penetration. This article aims to investigate and compare the effects of drug penetration enhancement through transdermal administration using vibrating microneedles versus low-frequency sonophoresis. Methods: In vitro permeation studies were conducted using Valia-Chien double chamber diffusion cells to evaluate the transdermal delivery of tetramethylpyrazine hydrochloride (TMPH). The TMPH concentration in the receiving compartment was determined using high-performance liquid chromatography (HPLC). Several combinations of microneedles and ultrasound settings were investigated, including different needle lengths, vibration frequencies, exposure times, and assorted distances of ultrasound horn and skin. Results:The results revealed the vibrating microneedle system as the most efficacious treatment to increase the TMPH permeability into the rat skin. The combination of a larger needle, higher frequency, and a 3-min exposure led to a 41.92-fold increase in cumulative permeability compared to the control group. The ultrasound treatment exhibited a moderate enhancement effect on TMPH skin penetration. Using a horn-toskin distance of 3 mm and a 3-min exposure resulted in a 4.34-fold increase in TMPH cumulative permeation compared to the control group. Conclusion: It could be concluded that while both the vibrating microneedle and the low-frequency ultrasound systems act as penetration enhancers for promoting the TMPH permeation through the skin, the vibrating microneedle system notably demonstrates a more effective penetration-promoting effect.