Comparative study of placental protein 19, human chorionic gonadotrophin and pregnancy-specific ?1-glycoprotein as immunohistochemical markers for extravillous trophoblast in pregnancy and trophoblastic disease

Abstract

PP19, a new placental tissue protein, has alpha 1-beta 1 electrophoretic mobility, a molecular weight of 36,500 and 3.9% carbohydrate. To study immunocytochemical PP19 localization in extravillous trophoblast, we obtained formalin-fixed specimens from extravillous tubal pregnancy at gestational weeks (GW) 7-9 (12 blocks); four early intrauterine pregnancies at GW 7-13 (12 blocks); four late pregnancies at GW 28-38 complicated with intramural uterine myoma, placenta increta and abruptio placenta (8 blocks); four invasive complete moles (9 blocks); and seven primary and metastatic gestational choriocarcinomas (12 blocks). Immunohistochemical staining was done for PP19, pregnancy-specific beta 1-glycoprotein (SP1) and human chorionic gonadotrophin (hCG) using the indirect-labeled antibody method [purified PP19 (Lot no. 225/242) and antibody against PP19 (Lot no. 632ZA) prepared by H. Bohn, antibodies against hCG (Behringwerke, Marburg, FRG) and SP1 (Dakopatts, Copenhagen, Denmark)]. In both early and late intrauterine pregnancies, the extravillous syncytiotrophoblastic cell (XST) showed positive staining for hCG and SP1 in the cytoplasm, as well as for PP19, which stained more intensively in the nucleus than in the cytoplasm. The three proteins were not seen in the evtravillous cytotrophoblastic cell (XCT) in the trophoblastic cell column and shell. The interstitial cytotrophoblast-like cell (ICT), which infiltrated into the decidua and myometrium, and their blood vessels, was immunoreactively positive for PP19 but negative for hCG and SP1 with the exception of SP1-positive ICT in the myometrium in late pregnancy. XST and ICT in the endosalpinx of tubal pregnancy stained for all three proteins.(ABSTRACT TRUNCATED AT 250 WORDS)

What Is Trophoblast? A Combination of Criteria Define Human First-Trimester Trophoblast

“…Multinucleated cells could be ST or placental bed giant cells. ST are HLA class I negative and express aminopeptidase A, placental leucine aminopeptidase, hCG, and pregnancy-specific glycoproteins ( Beck et al., 1986 , Takayama et al., 1989 , Zhou et al., 1997 , Hariyama et al., 2000 , Yamahara et al., 2000 , Ino et al., 2003 , Ito et al., 2003 ). Placental bed giant cells are strongly HLA-G + and hPL + ( Al-Lamki et al., 1999 ).…”

Concentration of placental protein 19 in body fluid and placental tissues

“…We clarified the localization of PP19 in the villous and extravillous trophoblast of the human placenta [2,3]. The purpose of this study was to clarify PP19 concentrations in different body fluids from pregnant women, nonpregnant women and men.…”