Comparative Study of Responses of Two Enzymatic Biosensors Based on Lipase from Candida rugosa and Porcine Pancreas for Detection of Diclofop-Methyl

Abstract

International audienceThe objective of this study was to evaluate the immobilization of lipase on an organic matrix by reticulation method, aiming at its application in the detection of organochlorine pesticide. The biologically inactive protein of bovine serum albumin (BSA) and glutaraldehyde were used for immobilizing lipase from a microbial source ( Candida rugosa CRL ) and animal cells (porcine pancreatic lipase, PPL). Use of this immobilized preparation was extended to the realization of two different origins of a miniaturized enzymatic biosensors is presented for the electrochemical detection of a large range of organochlorine pesticide (diclofop-methyl) in phosphate buffer saline (PBS) were observed experimentally. The best responses of biosensor were obtained for PPL with a high sensitivity to organochlorine pesticide in the concentration range of 5.99*10-12 to 5.89*10-2g*L-1, whereas a linear response of CRL for diclofop-methyl was obtained. The lipase biosensors exhibits the advantage of a simple fabrication procedure, wide dynamic range and a lower detection limit (5.99*10-12 g*L-1 for diclofop-methyl). The impact of heavy metals as well as organophosphorous and other organochlorine pesticides was also studied

Substrate-Specificity ofCandida rugosaLipase and Its Industrial Application

“…The biosensor presented good storage stability for 21 days when kept at 4 °C and it was successfully applied to real samples . Also another class, being organochlorine pesticides, can be detected with the use of a CRL biosensor . Not only pesticides can be detected with a biosensor, but also irritating or toxic (but useful) chemicals such as dimethyl phthalate.…”

“…128 Also another class, being organochlorine pesticides, can be detected with the use of a CRL biosensor. 132 Not only pesticides can be detected with a biosensor, but also irritating or toxic (but useful) chemicals such as dimethyl phthalate. The latter has many uses, e.g., in solid rocket propellants, plastics, and insect repellants, but direct exposure to dimethyl phthalate, via inhalation in humans and animals, results in irritation of the eyes, nose, and throat.…”