Comparative study of synthesized silver and gold nanoparticles using leaves extract of Bauhinia tomentosa Linn and their anticancer efficacy

Abstract

Nanotechnology is an emerging field in science and technology, which can be applied to synthesize new materials at the nanoscale level. The present investigation aimed at comparing the synthesis, characterization and in vitro anticancer efficacy of synthesized silver and gold nanoparticles using leaves extract of Bauhinia tomentosa Linn. Silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) were synthesized using aqueous extract of leaves with solution of silver nitrate (AgNO 3 , 1 mM) and chloroauric acid (HAuCl 4 •3H 2 O, 1 mM), respectively. The synthesized nanoparticles were characterized using UV-visible spectrophotometry, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, high-resolution transmission electron microscopy, energy-dispersive analysis of X-rays, X-ray diffraction, thermogravimetric analysis and cyclic voltammetry, which confirmed the reduction of Ag + ions to Ag 0 and Au 3+ ions to Au 0. The in vitro anticancer efficacy of AgNPs, AuNPs and aqueous extract of leaves confirmed by MTT assay exhibited IC 50 concentrations of 28.125, 46.875 and 50 μg ml −1 for lung A-549 cells, 103.125, 34.375 and 53.125 μg ml −1 for HEp-2 cells and 62.5, 23.4 and 13.26 μg ml −1 for MCF-7 cells, respectively. The concentrations indicate that both silver and gold nanoparticles as well as aqueous extract of leaves exhibited high anticancer efficacy.

The Activity of Gold Nanoparticles Synthesized Using Helichrysum odoratissimum Against Cutibacterium acnes Biofilms

“…The addition of gum arabic therefore plays a thermo-protective role for the nanoparticles. There were relatively low decreases in weight loss from 800 to 900°C which is most likely due to the remaining metallic gold residues ( Mukundan et al, 2017 ).…”

Green Synthesis of Silver Nanoparticles Using <em>Scindapsus officinalis</em> (Gajpipli): In-Vitro Cytotoxic Activity Against HepG-2 &amp; MCF-7 Cancer Cell Lines

“…Fourier Transform Infrared spectroscopy FT-IR is used to measure infrared absorption of the organic molecules found in the prepared samples. A range of 800-4000 cm−1 using Shimadzu (Mukundan et al, 2017). (He et al,…”

Seed Extract-mediated Synthesis of Silver Nanoparticles from Putranjiva roxburghii Wall., Phytochemical Characterization, Antibacterial Activity and Anticancer Activity Against MCF-7 Cell Line

“…The synthesized AgNPs exhibited dose-dependent cytotoxic effects on breast cancer cells in vitro [34] . AgNPs synthesised from Embelia ribes showed potential anticancer activity against MCF-7cells [35] , Bauhinia tomentosa against A-549, Hep-2 and MCF-7 cells [36] . AgNPs synthesized from plant extracts exhibited remarkable antitumour activity against MCF-7, HT29 and EAC cell lines [37] , the AgNPs from Salacia chinensis showed anticancer activity with IC 50 values ranging from 4.0 to 14.37 µg/ml against seven selected cancer cell lines [38] , also another report suggested that the AgNPs from plant extracts exhibited considerable cytotoxic activity against almost all tested human cancer cell lines [39] .…”