Obtaining transfer function of electrical, mechanical, etc. systems can provide this possibility for the researchers to investigate the behaviours of desired systems based on different inputs in various working circumstances without need to laboratory equipment which it results in lower consumption of time and expense. The aim of current research is obtaining the existing gyroscopic stabilizer transfer function. The way we used in this article is the newest laboratory way for obtaining transfer function of gyroscopic stabilizers. This aim is achieved by using laboratory equipment such as a two degrees of freedom gyro stabilized platform that an imaging system is installed on it as the load, target simulator table with one degree of freedom, and electronic conversion board of RS488 to RS232 serial communication standard, etc. An input which excites all modes (search and track) of two degrees of freedom gyro stabilized platform is introduced to under test system and the system behaviour toward the introduced input is saved and finally the transfer function of existing two degrees of freedom gyro stabilized platform is obtained using system identification toolbox in MATLAB. At the end of this article, the step response of transfer function obtained through the desired experiment in the laboratory compared with the step response obtained through simulations that a %10 difference between them is observed.