Comparative study of the anti-diabetic effect of mucilage and seed extract of Abelmoschus esculentus against streptozotocin-induced diabetes in rat model

Aleissa, Mohammed S.; Al-Zharani, Mohammed; Alneghery, Lina M.; Hasnain, Md Saquib; Almutairi, Bader O.; Ali, Daoud; Alarifi, Saud; Alkahtani, Saad

doi:10.1016/j.jksus.2022.102297

Cited by 10 publications

(12 citation statements)

References 20 publications

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“…In contrast, the reduced GSH levels in rats receiving both Theobroma cacao and Camellia sinensis warrant further investigation into how these substances interact with cellular antioxidant mechanisms. The present study's results revealed an increase in the GSH levels in diabetic rats after alloxan administration, consistent with previous research studies [16][17][18].…”

Section: Discussionsupporting

confidence: 93%

“…The diabetogenic action of alloxan causes toxicity in pancreatic cells due to excess ROS, resulting in decreased insulin production, release, and secretion while also affecting organs such as the liver, kidney, and hematopoietic system. Decreased antioxidant enzyme levels and enhanced lipid peroxidation are well-documented in alloxaninduced diabetes [12][13][14][15][16][17][18].…”

Section: Discussionmentioning

confidence: 99%

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Modulation of Oxidative Stress and Glycemic Control in Diabetic Wistar Rats: The Therapeutic Potential of Theobroma cacao and Camellia sinensis Diets

Indla,

Rajasekar,

Naveen Kumar

et al. 2024

Cureus

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BackgroundDiabetes mellitus is a complex metabolic disorder characterized by oxidative stress and impaired glycemic control. This study investigates the therapeutic potential of Theobroma cacao and Camellia sinensis diets in diabetic Wistar rats and assesses their impact on oxidative stress markers and blood glucose levels. MethodsIn this experiment, eight groups of six male Wistar rats (n = 12.5%), aged 8 to 12 weeks, were carefully set up to see how different treatments for diabetes and oxidative stress affected the two conditions. The random selection process was implemented to minimize any potential bias and ensure that the results of the study would be representative of the general population of Wistar rats. The groups were as follows: a nondiabetic control group (NDC) served as the baseline, while diabetes was induced in the alloxan monohydrate group (150 mg/kg). Another group was given the standard drug metformin (M, 100 mg/kg), and two control groups that did not have diabetes were given extracts of Theobroma cacao (TC, 340 mg/kg) and Camellia sinensis (CS, 200 mg/kg). Three groups of diabetic rats were given a mix of these treatments. Theobroma cacao and Camellia sinensis extracts were given at set doses (TC, 340 mg/kg; CS, 200 mg/kg), along with 150 mg/kg of a drug that causes diabetes. Over a 21-day period, oxidative stress parameters such as glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione reductase (GSHrd) levels, and blood glucose were carefully measured to check for signs of oxidative stress and diabetes progression ResultsConsiderable differences in GSH levels were noted across the groups, with the highest GSH concentration found in the group treated with the inducing drug, while the lowest GSH levels were observed in the diabetic group that was administered both Theobroma cacao and Camellia sinensis (p < 0.001). MDA levels also varied, with the diabetic group treated with Theobroma cacao having the highest MDA concentration (3.54 ± 0.29 μmol/L) and the nondiabetic control group treated with Camellia sinensis exhibiting the lowest MDA levels (1.66 ± 0.08 μmol/L; p < 0.001). SOD activity was highest in the standard drug group and lowest in the diabetic group treated with Theobroma cacao. GSH activity was notably higher in the diabetic groups that received dietary interventions (p < 0.001). Blood glucose levels showed diverse responses, with the standard drug group experiencing a substantial reduction, while the inducing drug group exhibited a consistent increase. ConclusionThe study highlights the significant impact of dietary interventions with Theobroma cacao and Camellia sinensis on oxidative stress markers and blood glucose regulation in diabetic Wistar rats. These findings suggest a potential role for these dietary components in mitigating oxidative stress and improving glycemic control in diabetes, although further research is warranted to elucidate the underlying mechanisms and clinical implications.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Modulation of Oxidative Stress and Glycemic Control in Diabetic Wistar Rats: The Therapeutic Potential of Theobroma cacao and Camellia sinensis Diets

Indla,

Rajasekar,

Naveen Kumar

et al. 2024

Cureus

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“…74 The abundance of quercetin in okra flesh contributed to its anti-inflammatory activity. 11 3.8.5. Effects of okra mucilage and flesh on liver and kidney parameters in HF/NaCl fed rats.…”

Section: Papermentioning

confidence: 99%

“…Free radicals can be neutralized by this plant's lipophilic and hydrophilic antioxidant components in both lipid and aqueous phases. 11 The flavonoids and vitamin C found in this plant work in concert to prevent the oxidation of low-density lipoproteins. 12 Additionally, numerous studies have shown that the antioxidant activity of okra peel and seed powder in diabetic rats could strengthen the pancreas, kidney, and liver.…”

Section: Introductionmentioning

confidence: 99%

Molecular docking and in vivo protective effects of okra (Abelmoschus esculentus) against metabolic dysfunction in high-fat, high-sodium diet-fed rats

Fouda,

Mohamed

2024

Food Funct.

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“…However, due to reduced CAT activity, beta cells of the pancreas that contain many mitochondria undergo oxidative stress by producing excess ROS that leads to β-cells dysfunction and, ultimately, diabetes [ 18 ]. Interestingly, evidence presented in Table 2 showed that Abelmoschus esculentus treatment in rodent models of diabetes significantly increases CAT activity [ 91 , 95 , 105 , 106 , 113 , 115 , 117 ]. This suggests that Abelmoschus esculentus may ameliorate oxidative stress, further reduce complications of DM, or prevent DM in non-diabetics ( Figure 4 ).…”

Section: Abelmoschus Esculentusmentioning

confidence: 99%

Comprehensive Overview of the Effects of Amaranthus and Abelmoschus esculentus on Markers of Oxidative Stress in Diabetes Mellitus

Phoswa,

Mokgalaboni

2023

Life

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The use of medicinal plants in the management of diabetes mellitus (DM) is extensively reported. However, there is still very limited information on the role of these plants as markers of oxidative stress in DM. This current review evaluated the effect of Amaranthus spinosus, Amaranthus hybridus, and Abelmoschus esculentus on markers of oxidative stress in rodent models of DM. Current findings indicate that these plants have the potential to reduce prominent markers of oxidative stress, such as serum malondialdehyde and thiobarbituric acid-reactive substances, while increasing enzymes that act as antioxidants, such as superoxide dismutase, catalase, glutathione, and glutathione peroxidase. This may reduce reactive oxygen species and further ameliorate oxidative stress in DM. Although the potential benefits of these plants are acknowledged in rodent models, there is still a lack of evidence showing their efficacy against oxidative stress in diabetic patients. Therefore, we recommend future clinical studies in DM populations, particularly in Africa, to evaluate the potential effects of these plants. Such studies would contribute to enhancing our understanding of the significance of incorporating these plants into dietary practices for the prevention and management of DM.

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Comparative study of the anti-diabetic effect of mucilage and seed extract of Abelmoschus esculentus against streptozotocin-induced diabetes in rat model

Cited by 10 publications

References 20 publications

Modulation of Oxidative Stress and Glycemic Control in Diabetic Wistar Rats: The Therapeutic Potential of Theobroma cacao and Camellia sinensis Diets

Modulation of Oxidative Stress and Glycemic Control in Diabetic Wistar Rats: The Therapeutic Potential of Theobroma cacao and Camellia sinensis Diets

Molecular docking and in vivo protective effects of okra (Abelmoschus esculentus) against metabolic dysfunction in high-fat, high-sodium diet-fed rats

Comprehensive Overview of the Effects of Amaranthus and Abelmoschus esculentus on Markers of Oxidative Stress in Diabetes Mellitus

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