Comparative Study of the Antioxidant Capacity of Four Stilbenes Using ORAC, ABTS+, and FRAP Techniques

Rodríguez-Bonilla, Pilar; Matencio, Adrián; García-Carmona, Francisco; López‐Nicolás, José Manuel

doi:10.1007/s12161-017-0871-9

Cited by 90 publications

(48 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fe (II) 9 . Briefly, Fe(III) was reduced to Fe(II), and Fe(II) was mixed with TPTZ to form a blue complex with strong absorption peak at 593 nm at pH = 3.6 condition.…”

Section: Dpph Assaymentioning

confidence: 99%

Structure-antioxidant activity relationship of methoxy, phenolic hydroxyl, and carboxylic acid groups of phenolic acids

Chen

Yang

et al. 2020

Sci Rep

411

232

View full text Add to dashboard Cite

The antioxidant activities of 18 typical phenolic acids were investigated using 2, 2′-diphenyl-1picrylhydrazyl (DPPH) and ferric ion reducing antioxidant power (FRAP) assays. Five thermodynamic parameters involving hydrogen atom transfer (HAT), single-electron transfer followed by proton transfer (SET-PT), and sequential proton-loss electron transfer (SPLET) mechanisms were calculated using density functional theory with the B3LYP/UB3LYP functional and 6-311++G (d, p) basis set and compared in the phenolic acids. Based on the same substituents on the benzene ring,-CH 2 cooH and-cH = CHCOOH can enhance the antioxidant activities of phenolic acids, compared with-COOH. Methoxyl (-ocH 3) and phenolic hydroxyl (-oH) groups can also promote the antioxidant activities of phenolic acids. These results relate to the O-H bond dissociation enthalpy of the phenolic hydroxyl group in phenolic acids and the values of proton affinity and electron transfer enthalpy (ETE) involved in the electron donation ability of functional groups. In addition, we speculated that HAT, SET-PT, and SPLET mechanisms may occur in the DPPH reaction system. Whereas SPLET was the main reaction mechanism in the FRAP system, because, except for 4-hydroxyphenyl acid, the ETE values of the phenolic acids in water were consistent with the experimental results. Phenolic acids, a class of compounds formed by the substitution of hydrogen atoms on benzene rings by a carboxylic acid group and at least one hydroxyl, are widely found in plants, plant foods, and human metabolites 1. Unlike flavonoids, free phenolic acids, such as benzoic, phenylacetic, and cinnamic acids, have high bioavailability and good water solubility 2. They can be absorbed in the stomach, whereas flavonoids cannot be absorbed, and only a small amount of flavonoids are transported passively through the intestinal wall into the blood 3-5. Most flavonoids are affected by pH, and digestive enzymes and intestinal microorganisms jointly affect C-ring cleavage, which breaks down into phenolic acids before being absorbed into the blood circulation system 6-8. Like flavonoids, phenolic acids are considered to be excellent antioxidants that can quench excessive free radical-induced body damage and chronic diseases 2. The antioxidant ability center of phenolic acids is phenolic hydroxyl, so the number and position of phenolic hydroxyls are directly related to their antioxidant activity 9. Moreover, the methoxy and carboxylic acid groups also have important effects on the antioxidant ability of phenolic acids 10,11. In recent years, with the development of computational chemistry based on density functional theory (DFT), theoretical results are often used to further explain the experimental results or predict the antioxidant activity of phenolic acids 12. Three key antioxidant mechanisms involved in the process of quenching free radicals are hydrogen atom transfer (HAT), single-electron transfer followed by proton transfer (SET-PT), and sequential proton-loss electron transfer (SPLET). HAT is a one-st...

show abstract

“…Fe (II) 9 . Briefly, Fe(III) was reduced to Fe(II), and Fe(II) was mixed with TPTZ to form a blue complex with strong absorption peak at 593 nm at pH = 3.6 condition.…”

Section: Dpph Assaymentioning

confidence: 99%

Structure-antioxidant activity relationship of methoxy, phenolic hydroxyl, and carboxylic acid groups of phenolic acids

Chen

Yang

et al. 2020

Sci Rep

411

232

View full text Add to dashboard Cite

show abstract

“…The antioxidative capacity of extracts of Norway spruce bark (70% EtOH) obtained from saw logs ( Figure 8 and Figure 9 ) and pulpwood ( Figure S1 ) were analyzed by using three different techniques (i.e., ORAC, FRAP, and SCAV), which differ in their objectives [ 35 ]. All the studied bark extracts posed high antioxidant power.…”

Section: Resultsmentioning

confidence: 99%

Fate of Antioxidative Compounds within Bark during Storage: A Case of Norway Spruce Logs

et al. 2020

View full text Add to dashboard Cite

Softwood bark is an important by-product of forest industry. Currently, bark is under-utilized and mainly directed for energy production, although it can be extracted with hot water to obtain compounds for value-added use. In Norway spruce (Picea abies [L.] Karst.) bark, condensed tannins and stilbene glycosides are among the compounds that comprise majority of the antioxidative extractives. For developing feasible production chain for softwood bark extractives, knowledge on raw material quality is critical. This study examined the fate of spruce bark tannins and stilbenes during storage treatment with two seasonal replications (i.e., during winter and summer). In the experiment, mature logs were harvested and stored outside. During six-month-storage periods, samples were periodically collected for chemical analysis from both inner and outer bark layers. Additionally, bark extractives were analyzed for antioxidative activities by FRAP, ORAC, and H2O2 scavenging assays. According to the results, stilbenes rapidly degraded during storage, whereas tannins were more stable: only 5–7% of the original stilbene amount and ca. 30–50% of the original amount of condensed tannins were found after 24-week-storage. Summer conditions led to the faster modification of bark chemistry than winter conditions. Changes in antioxidative activity were less pronounced than those of analyzed chemical compounds, indicating that the derivatives of the compounds contribute to the antioxidative activity. The results of the assays showed that, on average, ca. 27% of the original antioxidative capacity remained 24 weeks after the onset of the storage treatment, while a large variation (2–95% of the original capacity remaining) was found between assays, seasons, and bark layers. Inner bark preserved its activities longer than outer bark, and intact bark attached to timber is expected to maintain its activities longer than a debarked one. Thus, to ensure prolonged quality, no debarking before storage is suggested: outer bark protects the inner bark, and debarking enhances the degradation.

show abstract

“…(d) Equivalent antioxidant capacity (EAC) in Trolox-ABTS cation radical method or ABTS •+ (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Aldrich). The EAC in Trolox was evaluated according to the ABTS •+ method [45,47]. A solution with 40 mg of ABTS and 1.5 g of manganese dioxide (Fermont) was prepared in 15 mL of distilled water.…”

Section: Determination Of Nutraceutical Qualitymentioning

confidence: 99%

Interactive Effects of the Potassium and Nitrogen Relationship on Yield and Quality of Strawberry Grown Under Soilless Conditions

Rangel

Troyo-Diéguez²,

Valdéz-Aguilar

et al. 2020

Plants

View full text Add to dashboard Cite

K and N are the nutrients with the highest influence on yield and fruit quality. From this perspective, the aim of this study was to determine the effect of N as NO3−, K+ and their interactions on the yield and quality of strawberries grown under soilless conditions. A solution comprised of micronutrients based on an amended Steiner’s Universal Nutrient Solution was mixed with 4 levels of K+ (5, 7, 9 and 11 mol m−3) and 3 levels of NO3− (9, 12, and 15 mol m−3) to obtain 12 treatments. The results suggest that 15 mol m−3 of NO3− in the nutrient solution produced the highest yield, but fruit with low nutraceutical quality. On the other hand, 11 mol m−3 of K+ in the nutrient solution produced the highest yield and fruit with the best nutraceutical quality. The ionic concentration of the Universal Steiner’s Nutrient Solution proved to be the best nutritional option to maximize the yield and nutraceutical quality of strawberry fruit. The increase in NO3− concentration in the nutrient solution produced a higher yield of strawberries, while a higher concentration of K+ improved fruit quality, thus reaffirming the significance of nutrients within the plant functioning of this crop.

show abstract

Comparative Study of the Antioxidant Capacity of Four Stilbenes Using ORAC, ABTS+, and FRAP Techniques

Cited by 90 publications

References 37 publications

Structure-antioxidant activity relationship of methoxy, phenolic hydroxyl, and carboxylic acid groups of phenolic acids

Structure-antioxidant activity relationship of methoxy, phenolic hydroxyl, and carboxylic acid groups of phenolic acids

Fate of Antioxidative Compounds within Bark during Storage: A Case of Norway Spruce Logs

Interactive Effects of the Potassium and Nitrogen Relationship on Yield and Quality of Strawberry Grown Under Soilless Conditions

Contact Info

Product

Resources

About