Comparative study of the cellular fatty acids of methicillin-resistant and -susceptible<i>Staphylococcus aureus</i>

Asai, Shojiro; Noda, Mitsuhiro; Yamamura, Masatsugu; Hozumi, Yasukazu; Takase, Ichio; Nitta, Hiroshi; Sato, Minoru; Namikawa, Isamu

doi:10.1111/j.1699-0463.1993.tb00176.x

Cited by 7 publications

(6 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There has been some research about the cellular fatty acid compositions of S. aureus (Theodore & Panos ; Asai et al . ). Theodore and Panos reported that pentadecanoic acid (C15:0), heptadecanoic acid (C17:0) and nonadecanoic acid (C19:0) fatty acids were dominant in the plasma membrane of S. aureus (Theodore & Panos ).…”

Section: Discussionmentioning

confidence: 97%

“…The study about the cellular fatty acid composition of S. aureus showed that the major fatty acids were myristic acid (C14:0), C15:0, stearic acid (C18:0) and arachidic acid (C20:0) (Asai et al . ). It has been reported that the percentage of the C15:0 fatty acid component of MRSA strains was higher than that of MSSA strains (Asai et al .…”

Section: Discussionmentioning

confidence: 97%

“…It has been reported that the percentage of the C15:0 fatty acid component of MRSA strains was higher than that of MSSA strains (Asai et al . ). Asai et al .…”

Section: Discussionmentioning

confidence: 97%

See 2 more Smart Citations

Susceptibility difference between methicillin‐susceptible and methicillin‐resistant Staphylococcus aureus to a bovine myeloid antimicrobial peptide (BMAP‐28)

Takagi

Nishimura

Bai

et al. 2013

Animal Science Journal

View full text Add to dashboard Cite

A bovine myeloid antimicrobial peptide antimicrobial peptide (BMAP-28) is a member of the cathelicidin family and acts as a component of innate immunity. There are few reports of susceptibility difference of methicillin-resistant Staphylococcus aureus (MRSA) and susceptible strains (MSSA) against BMAP-28. This study aims to clarify how a few amino acid substitutions of BMAP-28 are related to its antimicrobial activity using four analog peptides of BMAP-28. We also compared cellular fatty acid components of MSSA and MRSA using gas chromatography. We found that a few amino acid substitutions of BMAP-28 do not change antimicrobial activity. It was also revealed that the percentage of cis-11-eicosenoic acid in total detected fatty acids of MRSA was significantly higher than that of MSSA. In addition, the percentage of palmitic acid in total detected fatty acids of MRSA tended to be lower than that of MSSA. Our results will provide new information to deal with the question of differences in bacterial susceptibility against BMAP-28.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Susceptibility difference between methicillin‐susceptible and methicillin‐resistant Staphylococcus aureus to a bovine myeloid antimicrobial peptide (BMAP‐28)

Takagi

Nishimura

Bai

et al. 2013

Animal Science Journal

View full text Add to dashboard Cite

show abstract

“…This identification system utilizes the type and amount of cellular fatty acids present in the lysate of an organism. Many species have unique cellular fatty acid chromatographic profiles [9,13]. Relationships between strains of a particular species may be inferred from highly similar cellular fatty acid profiles [7].…”

Section: Chromatographic Analysismentioning

confidence: 99%

Molecular Diagnostics of Infectious Diseases

Ohkusu

Ezaki²

2014

View full text Add to dashboard Cite

In DNA-based diagnostics, the polymerase chain reaction (PCR) is the most widely used DNA amplification method. To enable both sensitive and specific detection of agents causing infectious diseases, the PCR needs to be combined with methods to prepare the clinical sample containing the genetic material of the pathogen. Furthermore, methods for detection and DNA sequence analysis of the PCR amplification products are needed. This thesis describes the development of integrated systems for detection, quantification and characterization of microorganisms.An immunomagnetic separation (IMS) technique has been used to isolate Bordetella pertussis from nasopharyngeal aspirate samples. The post-PCR detection and typing of Bordetella spp. was performed by a combination of restriction enzyme analysis of the amplified pertussis toxin (PT) promoter region and a solid-phase colorimetric detection system; detection of immobilized amplified nucleic acid (DIANA). To investigate whether this approach could be used for reliable discrimination between the three Bordetella spp. infecting humans, the PT promoter region used for diagnostics was sequenced in 33 strains. To determine the DNA sequence of this polymorphic and repetitive region, a new technique, bidirectional pyrosequencing, was utilized. This procedure was used to resolve the sequence of this DNA region, which is able to form stable secondary structures in conventional Sanger DNA sequencing. A quantitative assay using competitive PCR and the DIANA detection technique was also developed, for quantification of B. pertussis in clinical samples.By arbitrary PCR, a DNA sequence apparently specific for Vibrio cholerae O139 Bengal was isolated and characterized. A nested PCR assay was developed for sensitive and specific detection of V. cholerae O139 Bengal in clinical samples and in environmental water samples, where differentiation between V. cholerae O139 Bengal and V. cholera O1 is of epidemiological interest.The magnetic separation approach was also used to capture human immunodeficiency virus (HIV-1) RNA from patient plasma. A nested reverse transcription (RT)-PCR with four internal competitors was combined with electrophoretic separation and quantification of the PCR amplicons on an automated DNA sequencer. From the internal calibration curve, the amount of HIV-1 RNA in the sample could be determined. Furthermore, a primer extension assay was combined with detection and quantification of the competitive PCR products by the same biochemiluminescent detection technique that is used in pyrosequencing. Quantification of HIV-1 viral load has implications in monitoring of antiretroviral therapy and in assessment of disease progression into AIDS. LIST OF PUBLICATIONSThis thesis is based on the following papers, which in the text will be referred to by their Roman numerals:

show abstract

“…The fatty acid profiles of LZ-01, LZ-02, LZ-03, LZ-04 and S. aureus type strains ATCC29213 and ATCC25923 [41,42] were presented in supplementary figure 3. The major fatty acids of the strains tested were C 15:0 iso, C 15:0 anteiso, C 16:0 , C 17:0 iso, C 17:0 anteiso and C 18:0 .…”

Section: Phylogenetic Analysis Of Isolated Strainsmentioning

confidence: 99%

A Novel Subspecies of Staphylococcus aureus from Sediments of Lanzhou Reach of the Yellow River Aerobically Reduces Hexavalent Chromium

Zhang¹,

Krumholz²,

Yu³

et al. 2013

J Bioremed Biodeg

View full text Add to dashboard Cite

Background: Lanzhou reach of the Yellow River is contaminated by heavy metals including chromium perennially. The microbial community within the sediment is very active. Yet the study on the bacteria in this distinctive microbial community is still scarce.Results: LZ-01, a Gram-positive hexavalent chromium-reducing bacterium was isolated from the soil sample collected at a petrochemical corporation wastewater discharge site of Lanzhou reach. It was able to aerobically reduce 94.5% of 0.4 mM Cr (VI) to Cr(III) in 120 hours. Cd (II) and NaN 3 treatment both repressed Cr(VI) reduction in LZ-01 and Cr(III) precipitates were detected both on the cell membrane and in the cytoplasm by Transmission Electron Microscopy (TEM) imaging. LZ-01 also demonstrated resistance to 4 mM As (V) and 9 mM U (VI). LZ-01 was closely related to Staphylococcus aureus revealed by 16S rRNA sequence analysis. Comparison of cellular fatty acid components and Vitek phenotype identification provided further evidences that LZ-01 is a novel subspecies of S. aureus. Conclusions:A chromate-reducing bacterium LZ-01 identified as a novel subspecies of S. aureus was isolated from Lanzhou reach of the Yellow River. Cd (II) and NaN 3 treatment and TEM images suggested that Cr(VI) was reduced not only intracellularly but also on the cell membrane. All the results indicate that the isolate has a great potential for bioremediation of Cr (VI)-contaminated environment. Staphylococcus aureus is a facultative Gram-positive coccus-shaped bacterium [27] that is best known for its ability to cause a range of illnesses [27,28]. Several recent studies report that S. aureus is involved in environmental metal remediation [4,29] and S. epidermidis which is closely related to S. aureus is able to anaerobically reduce Cr(VI) to Cr (III) [11]. However, there is no direct evidence that S. aureus can reduce Cr(VI). In this study, we screened the Lanzhou reach river bank soil for Cr(VI) reducers and isolated a novel subspecies of S. aureus strain LZ-01, which reduces Cr(VI) to Cr(III) aerobically. 16S rRNA sequencing and fatty lipids profiling showed that strain LZ-01 is closely related to S. aureus. Comparison of Vitek phenotype identification showed that there were 7 different reactions out of 64 between LZ-01 and type strains of Staphylococcus aureus. LZ-01 reduces Cr(VI) in a highly efficient manner suggesting that it may be useful for Cr(VI) reduction studies in Lanzhou reach. Journal of Bior emediation & Biodegradation Materials and Methods Soil sample collectionThe sampling site was 60 kilometers west of central Lanzhou city and adjacent to the upper reach of the Yellow River. The actual location was close to a petrochemical corporation waste water discharge site. The latitude and longitude of the sampling site is 36°06'N 103°39'E, (Figure 1). The pH of soils at the sampling site ranged from 5.0 to 6.0 with temperature around 18°C at the time of collection. The soil samples were collected from 15 cm below surface horizon and stored in sterile aluminum boxes a...

show abstract

Comparative study of the cellular fatty acids of methicillin-resistant and -susceptibleStaphylococcus aureus

Cited by 7 publications

References 26 publications

Susceptibility difference between methicillin‐susceptible and methicillin‐resistant Staphylococcus aureus to a bovine myeloid antimicrobial peptide (BMAP‐28)

Susceptibility difference between methicillin‐susceptible and methicillin‐resistant Staphylococcus aureus to a bovine myeloid antimicrobial peptide (BMAP‐28)

Molecular Diagnostics of Infectious Diseases

A Novel Subspecies of Staphylococcus aureus from Sediments of Lanzhou Reach of the Yellow River Aerobically Reduces Hexavalent Chromium

Contact Info

Product

Resources

About