Comparative study of the inhibitory effect of antidepressants on cholinesterase activity inBungarus sindanus(krait) venom, human serum and rat striatum

Abstract

Cholinesterases are divided into two classes based on differences in their substrate specificity and tissue distribution: acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). These enzymes may be inhibited by several compounds, such as antidepressants. The antidepressants paroxetine, imipramine, clomipramine and sertraline inhibited both venom AChE as well as human serum BChE in a concentration-dependent manner but had no effect on AChE in the rat brain striatum. The IC 50 of venom calculated for imipramine was 0.3 mM, paroxetine 0.38 mM, clomipramine 0.34 mM and sertraline 0.35 mM. Analysis of kinetic data indicated that the inhibition caused by sertraline and paroxetine was mixed, i.e. K m values increased and V max decreased in a concentration dependent manner. Imipramine and clomipramine exhibited competitive inhibition, i.e. K m values increased and V max remained constant. The present results suggest that these therapeutic agents used for depression can also be considered as inhibitors of snake venom and human serum cholinesterase.

Enhancing antioxidant, antidiabetic, and antialzheimer performance ofHippeastrum hybridum(L.) using silver nanoparticles

“…AChE activities were determined by the method of Ahmed et al [ 45 ] modified by Rocha et al [ 46 ] using a double beam spectrophotometer UV‐1602, BMS biotechnology medical service. Hydrolysis rates (V) were measured at various acetylthiocholine (S) concentrations (0.05–1 mM) in a 1 ml assay mixture with 50 mM phosphate buffer, pH 7.4, and 10 mM DTNB at 25°C.…”

Synthesis of biogenic silver nanoparticles from the extract ofHeliotropium eichwaldiL. and their effect as antioxidant, antidiabetic, and anti‐cholinesterase

“…The activity of AChE was deliberated by the Ahmed et al [ 40 ] method, which was improved by Rocha et al [ 41 ] Hydrolysis rates (V) of acetylthiocholine (S) of 1 mM were determined with phosphate buffer (50 Mm of pH 7.4), DTNB (10 mM), and 20 μl of AChE enzyme. The reaction mixture was followed by incubation at 37°C for 5 min.…”

Enzymatic and biochemical characterization of Bungarus sindanus snake venom acetylcholinesterase

“…Cholinesterase activities were determined according to Ahmed et al (20) with some modifications Rocha et al (21). Hydrolysis rates (V) were measured at various acetylthiocholine (S) concentrations (0.05 to 3 mM) in 1-mL assay solutions with 10 mM phosphate buffer, pH 7.5, and 0.2 mM DTNB at 25°C.…”