Comparative study of the lipogenic potential of human and rat adipose tissue

Świerczyński, Julian; Goyke, Elżbieta; Wach, Lidia; Pankiewicz, Areta; Kochan, Zdzisław; Adamonis, W.; Śledziński, Zbigniew; Aleksandrowicz, Z.

doi:10.1016/s0026-0495(00)80033-5

Cited by 47 publications

(32 citation statements)

References 21 publications

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“…Under normal conditions, the activity of ATP: citrate lyase, an important lipogenic enzyme, is very low in human AT. 22 A comparison of lipogenesis in the AT of rat and humans fed diets of similar composition has confirmed that the capacity of the lipogenic pathway of AT is lower in humans than in rats. This difference appears to be related to the lower abundance of mRNA (that is, lower expression) of the two transcription factors controlling the lipogenic pathways: sterol regulatory element-binding protein-1c and carbohydrate response element-binding protein.…”

Section: Introductionmentioning

confidence: 79%

Advances in adipose tissue metabolism

Lafontan

2008

Int J Obes

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This review will focus on the recent findings in adipose tissue metabolism with special attention to human adipocyte biology and physiology. There are major advances stemming from the concomitant results obtained from studies on mature human adipocytes, human preadipocytes differentiated in vitro and murine adipose cell lines. Physiological developments have been based on the expanded utilization of various kinds of murine transgenic models and physiological techniques such as microdialysis, open-flow microperfusion, arteriovenous techniques and the utilization of deuterium-or tritium-labelled metabolites that have provided a number of physiological advances in the understanding of human adipose tissue physiology. Gene expression profiling studies and nutrigenomics are emerging methods that herald interesting approaches for the future. An overview of recent discoveries in the mechanisms involved in the control of free fatty acid uptake, triacylglycerol synthesis and fat deposition will be discussed, as well as recent advances in the mechanisms involved in the lipolytic pathways, the role of lipases and perilipins. In addition, the in vivo validation of catecholamine action and the discovery of the lipolytic effects of natriuretic peptides will also be covered.

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Section: Introductionmentioning

confidence: 79%

Advances in adipose tissue metabolism

Lafontan

2008

Int J Obes

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“…In a recent study it was demonstrated that insulin, both alone and in combination with dexamethasone, increased human FASN gene expression and concomitantly elevated FASN activity [24]. Although the contribution of human adipose tissue to whole-body lipogenesis is considered to be low and less than that of liver [6,7], adipose tissue remains an important site of endogenous fatty acid synthesis [25]. Expression of the FASN gene is primarily regulated by hormonal and nutritional signals [26].…”

Section: Resultsmentioning

confidence: 99%

Fatty acid synthase gene expression in human adipose tissue: association with obesity and type 2 diabetes

et al. 2007

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Aims/hypothesis Increased expression and activity of the lipogenic pathways in adipose tissue may contribute to the development of obesity. As a central enzyme in lipogenesis, the gene encoding fatty acid synthase (FASN) was identified as a candidate gene for determining body fat. In the present study we tested the hypothesis that increased FASN expression links metabolic alterations of excess energy intake, including hyperinsulinaemia, dyslipidaemia and altered adipokine profile to increased body fat mass. Subjects and methods In paired samples of visceral and subcutaneous adipose tissue from 196 participants (lean or obese), we investigated whether FASN mRNA expression (assessed by PCR) in adipose tissue is increased in obesity and related to visceral fat accumulation, measures of insulin sensitivity (euglycaemic-hyperinsulinaemic clamp) and glucose metabolism.Results FASN mRNA expression was increased by 1.7-fold in visceral vs subcutaneous fat. Visceral adipose tissue FASN expression was correlated with FASN protein levels, subcutaneous FASN expression, visceral fat area, fasting plasma insulin, serum concentrations of IL-6, leptin and retinol-binding protein 4 (RBP4), and inversely with measures of insulin sensitivity, independently of age, sex and BMI. Moreover, we found significant correlations between FASN expression and markers of renal function, including serum creatinine and urinary albumin excretion. Conclusions/interpretation Increased FASN gene expression in adipose tissue is linked to visceral fat accumulation, impaired insulin sensitivity, increased circulating fasting insulin, IL-6, leptin and RBP4, suggesting an important role of lipogenic pathways in the causal relationship between consequences of excess energy intake and the development of obesity and type 2 diabetes.

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“…In addition to SREBP1, another transcriptional factor carbohydrate response element-binding protein (ChREBP) promotes de novo lipogenesis (DNL) gene expression and has been shown to modulate both lipid and glucose metabolism in adipose tissue and substantial whole-body insulin sensitivity (Herman et al 2012, Eissing et al 2013). However, under normal conditions, DNL is relatively low in WAT compared with liver and BAT in rodents and even lower in humans (Swierczynski et al 2000, Letexier et al 2003. The fatty acids used for TGs biosynthesis in adipocytes are 231:3 actually mainly from circulating, while glucose provides glycerol for esterifying fatty acids taken up from the circulating TGs in chylomicrons and very low-density lipoproteins (VLDL).…”

Section: Adipose Tissue As An Energy Storage Organmentioning

confidence: 99%

Adipose tissue in control of metabolism

Luo

Liu

2016

Journal of Endocrinology

506

331

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Adipose tissue plays a central role in regulating whole-body energy and glucose homeostasis through its subtle functions at both organ and systemic levels. On one hand, adipose tissue stores energy in the form of lipid and controls the lipid mobilization and distribution in the body. On the other hand, adipose tissue acts as an endocrine organ and produces numerous bioactive factors such as adipokines that communicate with other organs and modulate a range of metabolic pathways. Moreover, brown and beige adipose tissue burn lipid by dissipating energy in the form of heat to maintain euthermia, and have been considered as a new way to counteract obesity. Therefore, adipose tissue dysfunction plays a prominent role in the development of obesity and its related disorders such as insulin resistance, cardiovascular disease, diabetes, depression and cancer. In this review, we will summarize the recent findings of adipose tissue in the control of metabolism, focusing on its endocrine and thermogenic function.

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Comparative study of the lipogenic potential of human and rat adipose tissue

Cited by 47 publications

References 21 publications

Advances in adipose tissue metabolism

Advances in adipose tissue metabolism

Fatty acid synthase gene expression in human adipose tissue: association with obesity and type 2 diabetes

Adipose tissue in control of metabolism

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