Comparative study of the percutaneous permeation and bioaccumulation of a cyclic siloxane using frozen-thawed and nonfrozen ex vivo human skin

Mojsiewicz‐Pieńkowska, Krystyna; Krenczkowska, Dominika; Bazar, Dagmara; Wielgomas, Bartosz; Cal, Krzysztof; Kaliszan, Michał

doi:10.1016/j.tiv.2022.105379

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…We demonstrated in our team’s earlier work [ 47 ] that the freezing process has no impact on the skin’s barrier properties; therefore, the use of frozen–thawed samples of the biological material did not affect the results of this research. The integrity of the skin samples’ barrier was confirmed visually using a magnifier with 4-fold magnification to examine the upper side of the skin.…”

Section: Methodsmentioning

confidence: 90%

Investigating the Free Volumes as Nanospaces in Human Stratum Corneum Lipid Bilayers Using Positron Annihilation Lifetime Spectroscopy (PALS)

Mojsiewicz-Pieńkowska,

Bazar,

Filipecki

et al. 2024

IJMS

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This work is the first one that provides not only evidence for the existence of free volumes in the human stratum corneum but also focuses on comparing these experimental data, obtained through the unique positron annihilation lifetime spectroscopy (PALS) method, with theoretical values published in earlier works. The mean free volume of 0.269 nm was slightly lower than the theoretical value of 0.4 nm. The lifetime τ3 (1.83 ns with a coefficient of variation CV of 3.21%) is dependent on the size of open sites in the skin. This information was used to calculate the free volume radius R (0.269 nm with CV 2.14%), free volume size Vf (0.081 nm3 with CV 4.69%), and the intensity I3 (9.01% with CV 10.94%) to estimate the relative fractional free volume fv (1.32 a.u. with CV 13.68%) in human skin ex vivo. The relation between the lifetime of o-Ps (τ3) and the radius of free volume (R) was formulated using the Tao–Eldrup model, which assumes spherical voids and applies to sites with radii smaller than 1 nm. The results indicate that PALS is a powerful tool for confirming the existence of free volumes and determining their size. The studies also focused on describing the probable locations of these nanospaces in SC lipid bilayers. According to the theory, these play an essential role in dynamic processes in biological systems, including the diffusion of low-molecular-weight hydrophobic and moderately hydrophilic molecules. The mechanism of their formation has been determined by the molecular dynamics of the lipid chains.

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Section: Methodsmentioning

confidence: 90%

Investigating the Free Volumes as Nanospaces in Human Stratum Corneum Lipid Bilayers Using Positron Annihilation Lifetime Spectroscopy (PALS)

Mojsiewicz-Pieńkowska,

Bazar,

Filipecki

et al. 2024

IJMS

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“…In recent years, drug delivery of therapeutic agents has been revolutionized by encapsulating them into nanosized vesicular carrier systems. 1,2 These smart systems were reported very effective allowing to overcome limitations and problems associated with conventional drug delivery systems, 3,4 increasing attracting much attention among scientists worldwide. 5 Transdermal route is widely used to deliver chemical drugs and natural compounds to treat skin conditions (e.g., skin diseases, premature aging), because of its numerous advantages over oral (per os) route including, avoidance of first pass effects, decreased fluctuation in drug plasma level, (local) targeted delivery leading to lower side effects and increase efficiency, increased patient compliance.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, drug delivery of therapeutic agents has been revolutionized by encapsulating them into nanosized vesicular carrier systems 1,2 . These smart systems were reported very effective allowing to overcome limitations and problems associated with conventional drug delivery systems, 3,4 increasing attracting much attention among scientists worldwide 5 …”

Section: Introductionmentioning

confidence: 99%

Tocopherol succinate‐loaded ethosomal gel synthesized by cold method technique: Deeper biophysical characterizations for translational application on human skin

Akhtar,

Menaa,

Akhtar

et al. 2024

J of Cosmetic Dermatology

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BackgroundTocopherols are well‐known antioxidant and moisturizing agent. Tocopherol succinate (TS) are widely used in many skin products especially used in anti‐aging and skin whitening product formulation.AimWe previously reported the successful synthesis and preliminary characterizations of stable TS ethosomal gels (TSEG) (DOI: 10.1111/jocd.14907). Herein, we develop and further characterize TSEG to enhance the stability of the developed formulation with increased permeation through skin.MethodsCold method technique was used to prepare TS ethosomes. The developed ethosomal vesicle size was 250 nm, which allowed TS to penetrate through the stratum corneum layer and act on melanocytes. For stability study was assessed by thermogravimetric analysis (TGA) by placing TSEG and unloaded/control ethosomal gel (CEG) at various temperature conditions, that is, 8°C, 25°C, 40°C, and 40°C ± 75% RH for 3 months. Organoleptic evaluation was done in terms of color, odor, and phase separation. Transmission electron microscopy (TEM), Fourier Transform infrared spectroscopy (FTIR), x‐ray diffraction spectroscopy (XRD), zeta potential (ZP) and particle size (PS) was used for TSEG physical characterizations. In vitro dissolution and ex‐vivo permeation studies (using Franz diffusion cell) were performed for both TSEG and CEG formulations. Human women (N = 34) were used to evaluate in vivo biophysical parameters including erythema, melanin, moisture content, sebum level, and skin elasticity.ResultsDeveloped formulation was highly thermostable during the 3 months. Erythema, melanin, and sebum level decreased while marked improvement (p < 0.05) in moisture content and elasticity have been observed for the developed TSEG.ConclusionThe developed TSEG formulation was found to be efficient, safe (no adverse effects observed), stable (at least for 3 months), and easy to use for topical application with improved skin complexation and skin integrity.

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Characterization of a human lesioned-skin model to assess the influence of skin integrity on drug permeability

Viegas,

Dias,

Carvalho

et al. 2023

Biomedicine & Pharmacotherapy

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Comparative study of the percutaneous permeation and bioaccumulation of a cyclic siloxane using frozen-thawed and nonfrozen ex vivo human skin

Cited by 4 publications

References 31 publications

Investigating the Free Volumes as Nanospaces in Human Stratum Corneum Lipid Bilayers Using Positron Annihilation Lifetime Spectroscopy (PALS)

Investigating the Free Volumes as Nanospaces in Human Stratum Corneum Lipid Bilayers Using Positron Annihilation Lifetime Spectroscopy (PALS)

Tocopherol succinate‐loaded ethosomal gel synthesized by cold method technique: Deeper biophysical characterizations for translational application on human skin

Characterization of a human lesioned-skin model to assess the influence of skin integrity on drug permeability

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