Comparative Study of the Protective Effect of Cola anomala and Coffea arabica Against Induced Toxicity in Rats

Abstract

This work was aimed at evaluating the effects of C. anomala and C. arabica on Methotrexate (MTX) induced metabolic disorders. For this, the aqueous extract (AE) of C. anomala and C. arabica were prepared and their polyphenols, flavonoids and alkaloids contents determined as well as their antiradical and total antioxidant capacity. An animal experimentation using female rats was carried out for 14 days. Rats were divided into 6 groups; a negative control group receiving water; a positive control group receiving 12mg/Kg Bw of MTX; four tests groups receiving 12mg/Kg Bw of MTX and one of the extracts at a dose of 200mg/Kg Bw or 400mg/Kg Bw. At the end of the experiment, plasmas and hemolysates were prepared as well as liver and kidney homogenates for the evaluation of oxidative status (catalase, total protein and malondialdehyde (MDA)), liver toxicity (alanine amino transferase (ALT)) and renal toxicity (creatinine and urea) and lipid profile (triglycerides, total cholesterol and HDL-cholesterol). Weight gain in extract-treated rats was better with the C. anomala. Concerning oxidative status, MDA levels were generally lower in C. anomala-treated groups compared to C. arabica while catalase level was higher in C. anomala-treated rats. As for lipid profile, it is C. arabica that showed more or less better results. Both extracts led to an amelioration of toxicity markers compared to exclusive treatment with MTX. The results of this study suggest that C. anomala and C. arabica may reduce metabolic disorders associated with the intake of MTX during cancer treatment; C. anomala protecting better C. arabica.

Bioactive Compounds and Antioxidant Potential of Aqueous and Hydroethanolic Extracts of Cola lateritia Fruit Parts

“…Therefore, the selectivity of suitable solvents and extraction methods is important due to the various composition of phytochemicals. Most studies have demonstrated that methanol and ethanol solvents can extract more bioactive compounds in plants [14,15]. The differences between extraction methods (extraction conditions and extraction concentrations) not only influence the bioactive compound contents of extracts, but also affect their antioxidant potential.…”

“…In addition, the antioxidant activity of polyphenols and flavonoids depends on the number of hydroxyl groups in the molecule and its structure. A strong activity in the presence of low polyphenols and flavonoids contents may be explained by the fact that the other phytochemical compounds such as alkaloids and tannins, exhibited an antioxidant property [14,15,[19][20][21][22][23][24][25][26].…”