Comparative study of the role of serum levels of p53 antigen and its tumor cell concentration in colon cancer detection

A High Degree of Aneuploidy, Loss of p53 Gene, and Low Soluble p53 Protein Serum Levels Are Detected in Ulcerative Colitis Patients

“…The eluates were then separated in a high-pressure liquid chromatography (HPLC) apparatus (Merck Hitachi Co., Germany) equipped with a Vydac RP-18 column, essentially as previously described. [18][19][20] The system was equilibrated with a water-0.1 percent trifluoroacetic acid:isopropanol solution (90:10) at a flow rate of 0.5 ml/min. A linear gradient of 10 to 50 percent isopropanol in 0.1 percent trifluoroacetic acid, at the same flow rate, was run over 30 min, and 0.5-ml fractions were collected.…”

“…Pure p53 antigen isolated by gel-fiberglass columns was used as a standard and control as described previously. [18][19][20] Standardization included isolation of pure p53 antigen added to serum samples and determination of p53 concentration. We were able to accurately determine the concentration of added p53 antigen in the serum samples using the aforementioned extraction procedure, supporting the validity of the p53 serum levels obtained by this methodology (not shown).…”

A highly sensitive particle agglutination assay for the detection of P53 autoantibodies in patients with lung cancer

“…2 Furthermore, the assays in use for the detection of the p53 protein in the sera of patients with various forms of cancer demonstrate highly divergent results. [9][10][11][12] The use of different techniques and differences in the clinical status of the patients tested may explain part of the discrepancies observed. Whether these tests demonstrate false-negative results because of the presence of only trace amounts of antibodies and p53 protein, respectively, has, to our knowledge, not been addressed to date.…”

SERS-based nanobiosensing for ultrasensitive detection of the p53 tumor suppressor

“…1,2 The human p53 tumor suppressor is considered an indicator of special interest because its tissue and/or serum level has been associated with tumor invasiveness and prognosis in patients with various malignant diseases. [3][4][5][6][7] For this reason, several p53 detection procedures have been developed. [8][9][10][11][12][13][14][15][16] Undoubtedly, among these procedures, the most common ones are the traditional fluorescence-labeled immunological methods such as the enzyme-linked immunosorbent assay.…”

“…Such a property, coupled with high reproducibility, accuracy and speed, represents a starting point for further implementation in real biosensors. In this respect, we suggest that monitoring of p53 in serum could be extremely helpful as an indicator of prognosis and response to chemotherapy and radiotherapy in various malignant diseases, [3][4][5][6][7] and even to relate their insurgence to genotoxic environmental carcinogenic exposure.…”