Comparative study of water-soluble polysaccharides isolated from leaves and roots of Isatis indigotica Fort.

Pak, Un-hak; Yu, Yang; Ning, Xin; Ho, ChungHyok; Li, Ji; Mayo, Kevin H.; Zhou, Yifa; Sun, Lin

doi:10.1016/j.ijbiomac.2022.02.187

Cited by 18 publications

(13 citation statements)

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“…Two fractions of pectin (WRIP-A-A and WRIP-A-B) and one neutral polysaccharide (WRIP-N) were obtained from the roots of Isatis indigotica Fort. Nevertheless, the compositions of WRIP-A-A and WRIP-A-B were elucidated, but WRIP-N remained unexplained [ 40 ]. Notably, a crude polysaccharide has been extracted from Isatis indigotica Fort., consisting of Gal, Glu, Fuc, Xyl, Ara, Man, GalA and GluA [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

“…Isatidis Radix polysaccharides (RIP) are macromolecules that exhibit a range of biological activities, including combating the hepatitis B virus [ 37 ], improving obesity and acting as anti-oxidants and anti-inflammatory agents [ 38 , 39 ]. Despite the acquisition of various RIPs in recent years, the structural characteristics of neutral polysaccharides, their anti-inflammatory efficacy and the association between these traits remain unknown [ 40 , 41 ]. Thus, it was postulated that the neutral polysaccharide obtained and refined from the roots of Isatis indigotica Fort.…”

Section: Introductionmentioning

confidence: 99%

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The Isolation, Structural Characterization and Anti-Inflammatory Potentials of Neutral Polysaccharides from the Roots of Isatis indigotica Fort.

Shen,

Wu,

Song

et al. 2024

Molecules

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Polysaccharides have been assessed as a potential natural active component in Chinese herbal medicine with anti-inflammatory properties. However, the complex and indefinite structures of polysaccharides limit their applications. This study explains the structures and anti-inflammatory potentials of three neutral polysaccharides, RIP-A1 (Mw 1.8 × 104 Da), RIP-B1 (Mw 7.4 × 104 Da) and RIP-B2 (Mw 9.3 × 104 Da), which were isolated from the roots of Isatis indigotica Fort. with sequenced ultrafiltration membrane columns, DEAE-52 and Sephadex G-100. The planar structures and microstructures of RIP-A1, RIP-B1 and RIP-B2 were further determined by HPGPC, GC–MS, methylation analysis, FT-IR, SEM and AFM, in which the structure of RIP-A1 was elucidated in detail using 1D/2D NMR. The Raw 264.7 cells were used for the anti-inflammatory activity in vitro. The results showed that RIP-A1, RIP-B1 and RIP-B2 are all neutral polysaccharides, with RIP-A1 having the smallest Mw and the simplest monosaccharide composition of the three. RIP-A1 is mainly composed of Ara and Gal, except for a small quantity of Rha. Its main structure is covered with glycosidic linkages of T-α-Araf, 1,2-α-Rhap, 1,5-α-Araf, T-β-Galp, 1,2,4-α-Rhap, 1,3,5-α-Araf and 1,6-β-Galp with 0.33:0.12:1.02:0.09:0.45:11.41:10.23. RIP-A1 significantly inhibited pro-inflammatory cytokines (NO, TNF-α, IL-6 and IL-1β) and increased anti-inflammatory cytokines (IL-4) in LPS-stimulated RAW 264.7 cells. Moreover, RIP-A1 could significantly inhibit the mRNA expression of TNF-α, IL-6 and L-1β. It could also activate IKK, p65 and IκBα (the components of the NF-κB signaling pathway). In conclusion, the above results show the structural characterization and anti-inflammatory potentials of RIP-A1 as an effective natural anti-inflammatory drug.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Isolation, Structural Characterization and Anti-Inflammatory Potentials of Neutral Polysaccharides from the Roots of Isatis indigotica Fort.

Shen,

Wu,

Song

et al. 2024

Molecules

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“…Oligosaccharides with MW < 2 kDa were the major products. In addition, a small number of large and medium molecular weight fractions were also produced, which arise from RG-I and RG-II domains ( 34 , 35 , 40 ). Following degradation, hydrolysates were separated using Sephadex G-25 to remove polymers, thus yielding mono-/oligo-galacturonides (yield∼50–80%).…”

Section: Resultsmentioning

confidence: 99%

Determining Methyl-Esterification Patterns in Plant-Derived Homogalacturonan Pectins

Cui

Liu

et al. 2022

Front. Nutr.

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Homogalacturonan (HG)-type pectins are nutrient components in plants and are widely used in the food industry. The methyl-esterification pattern is a crucial structural parameter used to assess HG pectins in terms of their nutraceutical activity. To better understand the methyl-esterification pattern of natural HG pectins from different plants, we purified twenty HG pectin-rich fractions from twelve plants and classified them by their monosaccharide composition, Fourier transform-infrared spectroscopy (FT-IR) signatures, and NMR analysis. FT-IR shows that these HG pectins are all minimally esterified, with the degree of methyl-esterification (DM) being 5 to 40%. To examine their methyl-esterification pattern by enzymatic fingerprinting, we hydrolyzed the HG pectins using endo-polygalacturonase. Hydrolyzed oligomers were derivatized with 2-aminobenzamide and subjected to liquid chromatography-fluorescence-tandem mass spectrometry (HILIC-FLR-MSn). Twenty-one types of mono-/oligo-galacturonides having DP values of 1–10 were found to contain nonesterified monomers, dimers, and trimers, as well as oligomers with 1 to 6 methyl-ester groups. In these oligo-galacturonides, MSn analysis demonstrated that the number of methyl-ester groups in the continuous sequence was 2 to 5. Mono- and di-esterified oligomers had higher percentages in total methyl-esterified groups, suggesting that these are a random methyl-esterification pattern in these HG pectins. Our study analyzes the characteristics of the methyl-esterification pattern in naturally occurring plant-derived HG pectins and findings that will be useful for further studying HG structure-function relationships.

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“…It has the effects of clearing heat, detoxifying, and cooling blood, which can be used for wind-heat cold, epidemic encephalitis, mumps, and hepatitis [2,3]. Modern pharmacological research has confirmed that polysaccharides present in R. isatidis and F. isatidis show antibacterial, antiviral, and immuneenhancing biological activities [4][5][6], which are consistent with the efficacy of CBG. As a result, it is more scientific and reasonable to use polysaccharides analysis as an indicator to evaluate and control the quality of CBG.…”

Section: Introductionmentioning

confidence: 84%

Establishment and application of characteristic degradation fingerprint for the quality control of Compound Banlangen Granules polysaccharides

Liu

et al. 2023

J of Separation Science

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Compound Chinese medicine preparation is a complex multi-component system. The traditional methods such as physicochemical identification and quantification of several main index components cannot provide adequate quality evaluation for Compound Banlangen Granules. The objective of this work was to establish a characteristic degradation fingerprint of Compound Banlangen Granules polysaccharides, and the reference fingerprint was obtained from the model samples prepared using prescription medicinal herbs from different origins. The partial degradation products of Compound Banlangen Granules polysaccharides were profiled by capillary zone electrophoresis, and the quality difference of polysaccharides of these preparations was compared by cluster analysis and principal component analysis. It was found that the contents and the characteristic degradation fingerprints of the polysaccharides from 25 batches of Compound Banlangen Granules of 17 manufacturers were significantly different.The quality of Compound Banlangen Granules polysaccharides was evaluated by the characteristic degradation fingerprint tool with satisfactory results. The present method provides a reference for the quality control strategy development of polysaccharides in other compound Chinese medicine preparations.

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Comparative study of water-soluble polysaccharides isolated from leaves and roots of Isatis indigotica Fort.

Cited by 18 publications

References 50 publications

The Isolation, Structural Characterization and Anti-Inflammatory Potentials of Neutral Polysaccharides from the Roots of Isatis indigotica Fort.

The Isolation, Structural Characterization and Anti-Inflammatory Potentials of Neutral Polysaccharides from the Roots of Isatis indigotica Fort.

Determining Methyl-Esterification Patterns in Plant-Derived Homogalacturonan Pectins

Establishment and application of characteristic degradation fingerprint for the quality control of Compound Banlangen Granules polysaccharides

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