Comparative study of wound healing effect of topical Acacia catechu extract and silver sulfadiazine on excisional wound model in guinea pigs

Abstract

Background: Impaired and aberrant wound healing imposes a huge financial burden and places an enormous drain in health care resources in the developed world and an insurmountable problem in the developing countries too. In this study, authors have compared the wound healing effect of topical application of Acacia catechu extract with silver sulfadiazine in excisional wound model in guinea pigs.Methods: Twelve guinea pigs were randomly divided into two groups (n=6). The wound healing was observed in excision model. The standard group is treated with silver sulfadiazine (group 1) and the test group with Acacia catechu extract (group 2). The mean wound size expressed in mm2 and the mean percentage of the wound healed was measured on day 0, 4, 8, 12, 16 and 19. Biopsy was done on day 21 for histopathological examination (HPE).Results: Statistical analysis was done by using unpaired t test for between the groups comparison and by using paired t test for within the group comparison. The mean percentage of the wound healed with-in the groups was found to be statistically highly significant (p value <0.001) and in between the groups was found to be statistically non-significant (p value >0.05). A comparable increase in collagen content and granulation tissue was found on HPE in both the groups on day 21.Conclusions: Wound healing effect of the Acacia catechu extract is equal to and comparable with that of silver sulfadiazine.

Amazing Antimicrobial and Wound Healing Potential of Acacia Catechu Bark Extracts- A Review

“…To find out the responsible components of organic plant's extracts were separated by thin layer chromatography (TLC) and plant extracts were purified by column chromatography and were identified by Gas chromatography-mass spectrometry analysis. The analysis confirmed the presence of terpene, for example, camphor (nearly 76%) and phytol (nearly 28%) that to relate with its antibacterial and antifungal properties [28,32,36,37].…”

“…In few in-vitro evaluations too, the same is found to be exhibiting the antibacterial activity against enteric pathogens [30,31]. In species-specific anti-bacterial assays both ethanolic and aqueous extracts of the heartwood of A. catechu were seen to be successfully effective against Salmonella typhi, Shigella flexneri, Escherichia coli, Klebsiella pneumonia, Vibrio cholera, Pseudomonas aeruginosa and Staphylococcus aureus, etc bacterial cells [32][33][34]. In the same, the ethanolic extract was observed to be more efficient than the aqueous extract of the same also more efficient than the standard used [27,29,32].…”

Wound healing potential of Acacia catechu in streptozotocin-induced diabetic mice using in vivo and in silico approach

“…The present investigation was performed in order to investigate the potency of Acacia catechu bark for the treatment of diabetic wounds, since previous reports have revealed its promising wound healing and anti-diabetes potential. 9 , 10 , 11 The process of wound healing is complex and at the same time dynamic in nature comprising a sequence of various phases occurring within a specific time frame. 32 This process prerequisites coordination of various cellular activities such as phagocytosis, chemotaxis, angiogenesis, mitogenesis, collagen, and other matrix constituent's synthesis and various types of cells that participated in the healing process.…”

“… 7 , 8 Pharmacologically, it has been reported for its hypoglycaemic, anti-inflammatory, wound healing, antioxidant, antifungal, and antibacterial action. 9 , 10 , 11 Various phytoconstituents, that have been isolated from the plant includes catechin, (−) epicatechin, catechutannic acid, galocatechin, gallic acid, kaempferol, toxifolin, d -rhamnose, d -galactose, l -arabinose. Since the bark of A. catechu has been reported for its potent wound healing activity, therefore the present investigation was undertaken to evaluate a comparative wound healing potential of A. catechu bark extracts when administered orally and topically to streptozotocin-induced diabetic mice.…”

A Comprehensive Review on the Chemical Composition and Pharmacological Activities of Acacia catechu (L.f.) Willd.

“…e plant extract showed wound healing activities on the excisional wound model with a significant increase in collagen and granulation tissue on day 21 in guinea pigs [93].…”