Comparative Study of Ziehl-Neelsen Stain versus Fluorescent Microscopy in Diagnosis of Tuberculous Lymphadenitis on FNAC at A Tertiary Care Centre

Jadhav, Dnyaneshwar Shivajirao; Deshmukh, Dr.Vaibhav Vilas; Mahato, Nikhileshwar Haldhar; Valand, Arvind G

doi:10.17511/jopm.2019.i04.06

Cited by 4 publications

(1 citation statement)

References 11 publications

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“…In a study conducted by Annam et al 6 (2009) out of the 102 lymph nodes evaluated, maximum number of aspirates were from cervical lymph nodes (76/102) followed by inguinal nodes (11/102) and axillary nodes (15/102) Dnyaneshwar et al 7 (2019) revealed the most common site of involvement was cervical region with 27 out of 52 cases (51.92%), followed by supraclavicular region (17.31%) and axillary region (11.53%).…”

Section: Site Of Lymphadenopathymentioning

confidence: 99%

Comparison of CBNAAT and AFB screening using Ziehl Neelsen stain and fluorescent stain on FNAC sample for rapid diagnosis of tubercular lymphadenitis

Jayakumar,

Kaushal,

Duggal

2023

IJPO

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: Tuberculous lymphadenitis is the most common etiology of cervical lymphadenopathy in endemic countries. Fine needle aspiration of palpable lymph nodes is done for rapid diagnosis of tuberculous lymphadenitis This study presents a comparative evaluation of fine needle aspiration cytology (FNAC) with acid fast bacilli screening using Ziehl Neelsen stain, Fluorescent stain with Cartridge based nucleic acid amplification test (CBNAAT) for the rapid diagnosis of tubercular lymphadenitis.: An observational cross sectional study was done over a period of 15 months from January 2020 to March 2021. All newly diagnosed cases of tubercular lymphadenitis irrespective of age and sex were included. Fine needle aspiration was performed from the palpable lymph node. Smears were prepared using Giemsa, Papanicolaou, Ziehl Neelsen and Auramine O stain.Rest of the sample was used for Mycobacterial growth indicator test (MGIT) and CBNAAT. Statistical Analysis was performed using McNemar test. For gold standard, MGIT as well as a composite reference standard on parameters that included MGIT, radiological findings of tuberculosis, Positive Mantoux test and Positive response to ATT seen in the form of complete resolution of clinical and radiological findings.: The diagnostic value of CBNAAT differed with respect to the chosen gold standard. With MGIT as gold standard, CBNAAT had the highest sensitivity, specificity, positive predictive value and negative predictive value. The diagnostic accuracy of CBNAAT was also the highest. Using CRS (Composite Reference standard) as gold standard, CBNAAT showed the highest specificity and positive predictive value.: With CBNAAT showing statistically significant data of a higher diagnostic value in our study as well as showing rapid result, being automated and not subjected to observer interpretation, we conclude that CBNAAT is more efficient in the diagnosis of tubercular lymphadenitis as compared to AFB screening methods. The only limitation of CBNAAT in our study was its ability to show positive results for three atypical mycobacteria due to possible cross contamination.

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Section: Site Of Lymphadenopathymentioning

confidence: 99%

Comparison of CBNAAT and AFB screening using Ziehl Neelsen stain and fluorescent stain on FNAC sample for rapid diagnosis of tubercular lymphadenitis

Jayakumar,

Kaushal,

Duggal

2023

IJPO

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Comparative evaluation of Ziehl- Neelsen staining and kinyoun’s staining in the diagnosis of clinically suspected cases of tuberculosis

Chopra¹,

Chopra

Mahajan³

et al. 2022

IJMMTD

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: Tuberculosis commonly affects lungs but can also be extra-pulmonary. The increasing prevalence of extra-pulmonary manifestation of tuberculosis is a cause for concern. Microbiological diagnosis is essence for the effective treatment of mycobacterium tuberculosis. The smear detection tests differentiate infectious from non-infectious cases. Both Ziehl-Neelsen (Z-N) and kinyoun stain (KC) methods are over 100 years old; these are most economical in terms of processing time, needed equipment and personnel. The objective of research was to evaluate the two smear microscopy methods for the detection of tubercle bacilli in the sputum and Fine needle aspiration cytology (FNAC) material by comparing the sensitivity, specificity and validities of kinyoun’s method against Z-N staining.A prospective and comparative study was carried using 500 sputum samples and 100 FNAC material received in the department. All the 600 samples were subjected to two methods:Z-N stain and Kinyoun’s cold stain. They were compared for sensitivity and specificity in terms of qualitative results and grades of smear as recommended by RNTCP. : The smear positivity rate was 18% and 20.16% in Ziehl-Neelsen stain and Kinyoun’s cold stain respectively. The direct smear microscopy for Z-N staining showed sensitivity, 89.25%, specificity and positive predictive value (PPV) 100% and negative predictive value (NPV) 97.35% whereas KC method showed sensitivity 98.37%, specificity and PPV 100% and NPV 99.58% respectively. Kinyon’s method described is slightly longer to perform than the Ziehl-Neelsen technique but its ease of use and equal efficacy with Z-N method make it universally applicable, especially in those laboratories where facilities are poor.

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A Comparative Study of Ziehl-Neelsen Stain and Fluorescent Stain Microscopy in the Diagnosis of Pulmonary Tuberculosis

Masali¹,

Takpere²,

Shahapur³

2021

J. Pure Appl. Microbiol.

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Tuberculosis remains a global disease, killing over 2 million people each year. India accounts for two-third of the global total i.e. 26%. A good, fast, and economical test to diagnose a disease is urgently needed so that effective treatment can begin as soon as possible. Various investigations can be done for TB diagnosis and these include clinical suspicion, Chest imaging, acid-fast bacilli staining, MTB culture, serological methods and Assays for amplification of nucleic acids. For developing countries like India due to the enormous number of patients and budget restrictions, the assessment of a quick and low-cost diagnostic procedure is critical. A present prospective study was undertaken to compare Ziehl-Neelsen stain with Fluorescent stain microscopy in detecting of Mycobacterium tuberculosis in TB patient’s sputum samples. Among 274 sputum samples collected from suspected TB cases, 50 (18.2%) were positive by ZN and 116 (42.3%) were positive by fluorescent microscopy. The sensitivity, specificity, Positive predictive value (PPV) & Negative predictive value (NPV) of FM microscopy was 98%, 70%, 42%, and 99% respectively. FM microscopy was superior to ZN microscopy in detecting pulmonary tuberculosis cases as it could detect 24.1% more positive cases than ZN staining. Fluorescence staining provides better sensitivity and specificity and can detect accurately more paucibacillary cases hence is of diagnostic value and can help in the early treatment of pulmonary tuberculosis.

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Comparative Study of Ziehl-Neelsen Stain versus Fluorescent Microscopy in Diagnosis of Tuberculous Lymphadenitis on FNAC at A Tertiary Care Centre

Cited by 4 publications

References 11 publications

Comparison of CBNAAT and AFB screening using Ziehl Neelsen stain and fluorescent stain on FNAC sample for rapid diagnosis of tubercular lymphadenitis

Comparison of CBNAAT and AFB screening using Ziehl Neelsen stain and fluorescent stain on FNAC sample for rapid diagnosis of tubercular lymphadenitis

Comparative evaluation of Ziehl- Neelsen staining and kinyoun’s staining in the diagnosis of clinically suspected cases of tuberculosis

A Comparative Study of Ziehl-Neelsen Stain and Fluorescent Stain Microscopy in the Diagnosis of Pulmonary Tuberculosis

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