Comparative Study on Anti-Inflammatory Effect of Polysaccharides from Vinegar-Baked <i>Radix Bupleuri</i> Using Different Methods

Wei, Ni; Wang, Xiaoshuang; Wu, Yayun; Liu, Lijuan; Zhao, Ya; Zhao, Ruizhi

doi:10.1021/acsomega.3c02590

Cited by 5 publications

(2 citation statements)

References 38 publications

(81 reference statements)

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“…Findings suggest that Gal-UA may have anti-inflammatory effects and play a role in immune response regulation. Additionally, the amount of Ara in polysaccharides is positively associated with their immune activity [ 35 ]. CPTM-P1 contains elevated levels of both Gal-UA and Ara, which could potentially account for the observed immune activity of CPTM-P1.…”

Section: Resultsmentioning

confidence: 99%

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Fan,

Huang,

Dou

et al. 2024

Molecules

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Polysaccharides extracted from Taxus media hrough an aqueous method were further refined by removing proteins via the Sevag technique and purified by dialysis. The separation of these polysaccharides was accomplished using a DEAE-cellulose chromatog-raphy column, yielding two distinct fractions, named CPTM-P1 and CPTM-P2. Notably, CPTM-P1 emerged as the primary polysaccharide component within Taxus media. Consequently, a comprehensive analysis focusing exclusively on CPTM-P1 was undertaken. The molecular weight of CPTM-P1 was established through gel permeation chromatography (GPC), and its monosaccharide composition was deciphered using HPLC-MS. The structure was further elucidated through nuclear magnetic resonance (NMR) spectroscopy. The molecular weight of CPTM-P1 was determined to be 968.7 kDa. The monosaccharide composition consisted of galactose (Gal), arabinose (Ara), galacturonic acid (Gal-UA), glucose (Glc), rhamnose (Rha), xylose (Xyl), mannose (Man), fucose (Fuc), glucuronic acid (Glc-UA), and ribose (Rib). The proportional distribution of these components was 30.53%, 22.00%, 5.63%, 11.67%, 11.93%, 1.69%, 8.50%, 1.23%, 5.63%, and 1.17%, respectively. This confirmed CPTM-P1 as an acidic heteropolysaccharide with a glycuronic acid backbone. Moreover, CPTM-P1 showed immunoenhancing properties, effectively augmenting the secretion of nitric oxide and cytokines (TNF-α, IL-1β, and IL-6). Additionally, it significantly enhances the phagocytic capacity of RAW264.7 cells. These findings underscore the potential application of these polysaccharides in functional foods and pharmaceuticals, providing a solid scientific basis for further exploration and utilization of Taxus media polysaccharides.

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Section: Resultsmentioning

confidence: 99%

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Fan,

Huang,

Dou

et al. 2024

Molecules

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“…The monosaccharide content in the AKUs was determined by complete acid hydrolysis combined with PMP precolumn derivatization. 26 In short, 5 mg of dried AKUs was hydrolyzed with 4 mL of trifluoroacetic acid (TFA, 4 M) at 110 °C for 4 h under a nitrogen atmosphere. After that, the sample was mixed with equal volume 0.6 mol/L 1-phenyl-3-methyl-5-pyrazolone (PMP) and 0.3 mol/L NaOH solution and incubated at 70 °C for 100 min.…”

Section: Methodsmentioning

confidence: 99%

Ultrasound-Assisted Extraction of Atractylodes chinensis (DC.) Koidz. Polysaccharides and the Synergistic Antigastric Cancer Effect in Combination with Oxaliplatin

Liang,

Wu,

Sun

et al. 2024

ACS Omega

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Oxaliplatin (OXA) is recognized as a first-line drug for gastric cancer. However, low accumulation of the OXA in the target site and the development of drug resistance directly led to treatment failure. In the present study, an ultrasonic extraction method for Atractylodes chinensis (DC.) Koidz. polysaccharides (AKUs) and the combination treatment with OXA in vitro were studied. Results showed that when the pH level was 11, the ultrasound power at 450 W, the solid−liquid ratio was 1:20, and the ultrasound treatment for 30 min, the yield of AKUs was significantly increased to 13.20 ± 0.35%. The molecular weights of the AKUs ranged from 7.21 to 185.94 kDa, and its monosaccharides were mainly composed of arabinose (Ara), galactose (Gal), and glucose (Glu) with a ratio of 58.36, 16.90, and 15.49%, respectively. Cell experiments showed that, compared to OXA alone (2 μg/mL, inhibition rate of 18%), the treatment of OXA with AKUs had a significant synergistic inhibitory effect on MKN45 proliferation, which increased to 33, 41, and 45% with increasing AKUs concentrations (5−50 μg/mL), respectively, representing a 2.5-fold inhibition. Inductively coupled plasma-mass spectrometry (ICP-MS) determination confirmed that AKUs significantly increased the intracellular uptake of OXA by 29%, compared to that of OXA alone. We first demonstrated that the combined synergistic inhibitory effect of AKUs and OXA on gastric cancer cells was mediated by reducing the expression of efflux proteins (MRP1 and MRP2) and increasing the expression of ingested protein (OCT2). As a result of the above, AKUs deserved to be an effective adjuvant combined with chemotherapeutics in a clinical setting.

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Anti-inflammation mechanisms of a homogeneous polysaccharide from Phyllanthus emblica L. on DSS induced colitis mice via the gut microbiota and metabolites alteration

Li,

Chen,

Peng

et al. 2024

Food Chemistry

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Comparative Study on Anti-Inflammatory Effect of Polysaccharides from Vinegar-Baked Radix Bupleuri Using Different Methods

Cited by 5 publications

References 38 publications

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Ultrasound-Assisted Extraction of Atractylodes chinensis (DC.) Koidz. Polysaccharides and the Synergistic Antigastric Cancer Effect in Combination with Oxaliplatin

Anti-inflammation mechanisms of a homogeneous polysaccharide from Phyllanthus emblica L. on DSS induced colitis mice via the gut microbiota and metabolites alteration

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