Comparative study on antimicrobial activity of mono-rhamnolipid and di-rhamnolipid and exploration of cost-effective antimicrobial agents for agricultural applications

Zhao, Feng; Wang, Bingxin; Yuan, Menglin; Ren, Shi‐Bin

doi:10.1186/s12934-022-01950-x

Cited by 30 publications

(7 citation statements)

References 35 publications

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“…Reports on the antimicrobial activity of BS by P. aeruginosa against Gram-positive and Gram-negative bacteria and yeast investigated in this research, as well as other microorganisms of industrial and medical importance, have already been described in the literature 57 , 58 . Gram-positive bacteria usually are more susceptible to BS 59 , whose inhibition mechanism occurs through the insertion of fatty acid components (short acyl tails) into the bacterial cell membrane, causing a break between the cytoskeletal elements and the plasma membrane 57 , 58 . However, Gram-negative bacteria are normally more resistant to BS, possibly due to the presence of lipopolysaccharide and extracellular polymers in the outer membrane, which is little permeable to hydrophobic and amphipathic molecules 59 .…”

Section: Discussionmentioning

confidence: 99%

“…Gram-positive bacteria usually are more susceptible to BS 59 , whose inhibition mechanism occurs through the insertion of fatty acid components (short acyl tails) into the bacterial cell membrane, causing a break between the cytoskeletal elements and the plasma membrane 57 , 58 . However, Gram-negative bacteria are normally more resistant to BS, possibly due to the presence of lipopolysaccharide and extracellular polymers in the outer membrane, which is little permeable to hydrophobic and amphipathic molecules 59 . The weak antifungal activity can be attributed to the BS composition, as suggested by Rodrigues et al 60 ; compared to di-RL, mono-RL congeners interact weakly with the fungal phospholipid bilayer, and in our heterogeneous mixture of rhamnolipids, the ratio of mono-RL is higher.…”

Section: Discussionmentioning

confidence: 99%

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Production and characterization of rhamnolipids by Pseudomonas aeruginosa isolated in the Amazon region, and potential antiviral, antitumor, and antimicrobial activity

Cerqueira dos Santos,

Araújo Torquato,

de Alexandria Santos

et al. 2024

Sci Rep

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Biosurfactants encompass structurally and chemically diverse molecules with surface active properties, and a broad industrial deployment, including pharmaceuticals. The interest is growing mainly for the low toxicity, biodegradability, and production from renewable sources. In this work, the optimized biosurfactant production by Pseudomonas aeruginosa BM02, isolated from the soil of a mining area in the Brazilian Amazon region was assessed, in addition to its antiviral, antitumor, and antimicrobial activities. The optimal conditions for biosurfactant production were determined using a factorial design, which showed the best yield (2.28 mg/mL) at 25 °C, pH 5, and 1% glycerol. The biosurfactant obtained was characterized as a mixture of rhamnolipids with virucidal properties against Herpes Simplex Virus, Coronavirus, and Respiratory Syncytial Virus, in addition to antimicrobial properties against Gram-positive bacteria (Staphylococcus aureus and Enterococcus faecium), at 50 µg/mL. The antitumor activity of BS (12.5 µg/mL) was also demonstrated, with potential selectivity in reducing the proliferation of breast tumor cells, after 1 min of exposure. These results demonstrate the importance of studying the interconnection between cultivation conditions and properties of industrially important compounds, such as rhamnolipid-type biosurfactant from P. aeruginosa BM02, a promising and sustainable alternative in the development of new antiviral, antitumor, and antimicrobial prototypes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Production and characterization of rhamnolipids by Pseudomonas aeruginosa isolated in the Amazon region, and potential antiviral, antitumor, and antimicrobial activity

Cerqueira dos Santos,

Araújo Torquato,

de Alexandria Santos

et al. 2024

Sci Rep

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“…B, Actinomucor sp. Y and Penicillium oxalicum S11, and even against certain bacteria such as Escherichia coli DH5α, Bacillus wiedmannii H238, B. Safensis B36# and Pantoea agglomerans B10 [40,41]. A comparison of mRLs and dRLs from different producers showed that defense gene expression induction in grapevine cells is highly individual in response to the same concentration of mRLs or dRLs from P. aeruginosa (predominant C10-C10 acyl chain) or dRLs from B. plantarii (predominant C14-C14 acyl chain) [12].…”

Section: Discussionmentioning

confidence: 99%

The biological activity of bacterial rhamnolipids is linked to their molecular structure

Bredenbruch

Mueller

Atemnkeng

et al. 2023

Preprint

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Biosurfactants are amphiphilic compounds of microbial origin with a wide range of industrial applications. Rhamnolipids (RLs) offer a broad range of potential applications as biosurfactants in industry and agriculture. Several studies report a high capacity for controlling different plant pests and pathogens and consider RLs as promising candidates for bio-based plant protection agents. RLs are a class of glycolipids consisting of one (mRLs) or two (dRLs) rhamnose moieties linked to a single or branched β fatty acid. Due to the diversity of RLs, so far little is known about the relation between molecular structure and biological activity. Engineering the synthesis pathway allowed us to differentiate between the activities of mixtures of pure mRL and of pure dRL congeners and elaborated HPLC techniques further enabled to analyse the activity of single congeners. In a model system with the plant Arabidopsis thaliana and the plant-parasitic nematode Heterodera schachtii we demonstrate that RLs can significantly reduce infection, whereas their impact on the host plant varies depending on their molecular structure. While mRLs reduced plant growth even at low concentration, dRLs showed no or a beneficial impact on plant development. In addition, the effect of RLs on plant H2O2 production was measured as an indicator of plant defense activity. Treatment with mRLs or dRLs at a concentration of 50 ppm increased H2O2 production. Lower concentrations of up to 10 ppm were used to stimulate plants prior to a treatment with water or flagellin (flg22), a bacterial inducer of plant defense responses. At 10 ppm both mRLs and dRLs fostered an increased response to flg22. However, mRLs also led to an increased response to water, the non-inducing negative control, indicating a generally elevated stress level. Neither mRLs nor dRLs induced expression of plant defense marker genes of salicylic acid, jasmonic acid and ethylene pathways within a 1 hour and 48 hours treatment. Due to the negative effect of mRLs on plants further studies were concentrated on dRLs. Treatment of pre-parasitic infective juveniles of H. schachtii revealed that dRLs did not increase mortality even at a very high concentration of 755 ppm. In order to analyse the effect of single dRL congeners nematode infection assays were performed. While dRL congeners with a C10-C8 acyl chain increased nematode infection, dRLs with C10-C12 and C10-C12:1 acyl chains reduced nematode infection even at concentrations below 2 ppm. Plant growth was not reduced by C10-C8 dRLs, but by C10-C12 and C10-C12:1 dRLs at concentrations of 8.3 ppm. H2O2 production was increased compared to the water control upon treatment with C10-C8 dRLs at a concentration of 200 ppm, while C10-C12 and C10-C12:1 dRLs triggered the same effect already at 50 ppm. Our experiments show a clear structure-effect relation. In conclusion, functional assessment and analysis of mode of action of RLs in plants require careful consideration of their molecular structure and composition.

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“…In agriculture, RLs effectively take part in disease management by diminishing the growth of phytopathogens. These biosurfactants also have antiviral, antifungal, and antibacterial potential . The benefits and market potential of RLs have been extensively studied, , e.g., the virucidal effects of RLs were recalled when COVID-19 emerged as global issue .…”

Section: Pseudomonas Species Producing Rhamnolipids (Biosurfactants)mentioning

confidence: 99%

Multifaceted Impacts of Plant-Beneficial Pseudomonas spp. in Managing Various Plant Diseases and Crop Yield Improvement

Mehmood,

Saeed,

Zafarullah

et al. 2023

ACS Omega

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The modern agricultural system has issues with the reduction of agricultural productivity due to a wide range of abiotic and biotic stresses. It is also expected that in the future the entire world population may rapidly increase and will surely demand more food. Farmers now utilize a massive quantity of synthetic fertilizers and pesticides for disease management and to increase food production. These synthetic fertilizers badly affect the environment, the texture of the soil, plant productivity, and human health. However, agricultural safety and sustainability depend on an ecofriendly and inexpensive biological application. In contrast to synthetic fertilizers, soil inoculation with plant-growth-promoting rhizobacteria (PGPR) is one of the excellent alternative options. In this regard, we focused on the best PGPR genera, Pseudomonas, which exists in the rhizosphere as well as inside the plant’s body and plays a role in sustainable agriculture. Many Pseudomonas spp. control plant pathogens and play an effective role in disease management through direct and indirect mechanisms. Pseudomonas spp. fix the amount of atmospheric nitrogen, solubilize phosphorus and potassium, and also produce phytohormones, lytic enzymes, volatile organic compounds, antibiotics, and secondary metabolites during stress conditions. These compounds stimulate plant growth by inducing systemic resistance and by inhibiting the growth of pathogens. Furthermore, pseudomonads also protect plants during different stress conditions like heavy metal pollution, osmosis, temperature, oxidative stress, etc. Now, several Pseudomonas-based commercial biological control products have been promoted and marketed, but there are a few limitations that hinder the development of this technology for extensive usage in agricultural systems. The variability among the members of Pseudomonas spp. draws attention to the huge research interest in this genus. There is a need to explore the potential of native Pseudomonas spp. as biocontrol agents and to use them in biopesticide development to support sustainable agriculture.

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Comparative study on antimicrobial activity of mono-rhamnolipid and di-rhamnolipid and exploration of cost-effective antimicrobial agents for agricultural applications

Cited by 30 publications

References 35 publications

Production and characterization of rhamnolipids by Pseudomonas aeruginosa isolated in the Amazon region, and potential antiviral, antitumor, and antimicrobial activity

Production and characterization of rhamnolipids by Pseudomonas aeruginosa isolated in the Amazon region, and potential antiviral, antitumor, and antimicrobial activity

The biological activity of bacterial rhamnolipids is linked to their molecular structure

Multifaceted Impacts of Plant-Beneficial Pseudomonas spp. in Managing Various Plant Diseases and Crop Yield Improvement

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