Comparative study on coagulant, defibrinating, fibrinolytic and fibrinogenolytic activities of Costa Rican crotaline snake venoms and their neutralization by a polyvalent antivenom

Gené, JoséAntonio; Roy, Astrid; Rojas, Gustavo; Gutiérrez, José Marı́a; Cerdas, Luis

doi:10.1016/0041-0101(89)90096-2

Cited by 186 publications

(75 citation statements)

References 9 publications

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“…Mice injected i. v. with SS only had a whole blood clotting time of 121 ± 56 sec, whereas mice receiving B. asper venom developed a characteristic dose-dependent defi brination syndrome, as previously described [30]. Doses of 1.5 and 3.0 µg did not prolong the clotting time when compared with mice receiving SS alone (112 ± 34 sec and 88 ± 14 sec, respectively; P > 0.05).…”

Section: Effects Of Inhibition Of No Synthesis In Systemic Alterationssupporting

confidence: 74%

Role of nitric oxide in the local and systemic pathophysiological effects induced by Bothrops asper snake venom in mice

2006

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Abstract.Objective: To assess the role of nitric oxide in the most relevant local and systemic manifestations in mice injected with the venom of the snake Bothrops asper. Mice were pretreated with nitric oxide synthase inhibitors, and the modifi cations of the pathological effects induced by the venom were tested. Results: Inhibition of NO synthesis did not affect acute local myonecrosis and hemorrhage in muscle tissue upon intramuscular injection of venom. Local footpad edema was reduced in mice pretreated with the NO synthase inhibitor L-NAME, and a reduction in the extent of infl ammatory infi ltrate in muscle tissue was observed after envenomation in mice pretreated with L-NAME and aminoguanidine. The most pronounced effect of NOS inhibition by L-NAME was an increment in the lethal activity of the venom, when injected by the intraperitoneal route. Conclusion: Nitric oxide does not seem to play a signifi cant role in the local acute pathological alterations (hemorrhage and myonecrosis) induced by B. asper venom in mice, although it contributes to edema and infl ammatory infi ltrate. Nitric oxide exerts a protective role in the systemic pathophysiological manifestations leading to lethality.

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Section: Effects Of Inhibition Of No Synthesis In Systemic Alterationssupporting

confidence: 74%

Role of nitric oxide in the local and systemic pathophysiological effects induced by Bothrops asper snake venom in mice

2006

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“…The method described by the Gene et al [12] was followed with slight modification. A mixture consisting 2 ml of healthy human plasma and 3 ml of 1.2% molten agarose (45°C) in 10 mM Tris-HCl buffer containing 0.02% sodium azide, 70 mM (NH 4 ) 2 SO 4 , 90 mM NaCl, 0.70 mM MgCl 2 and 200 ll of 0.2 M CaCl 2 was poured into 10 9 90 mm Petri dish and allowed for 2 h at 27°C.…”

Section: Plate Methodsmentioning

confidence: 99%

Anti-coagulant activity of a metalloprotease: further characterization from the Indian cobra (Naja naja) venom

Kumar

Devaraj

Vishwanath

et al. 2009

J Thromb Thrombolysis

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A high molecular mass, non toxic metalloprotease the NN-PF3 with the bound Ca(2+) and Zn(2+) from the Naja naja venom has been studied further for its anticoagulant property. The molecular mass by MALDI-TOF mass spectrometry was 67.81 kDa. The NN-PF3 exhibited fibrin(ogen)olytic activity. In addition to fibrinogen, NN-PF3 hydrolyzed blood and plasma clot with the later hydrolyzed about one fold higher. The alpha polymer of fibrin was preferentially hydrolyzed over the alpha chain but the beta chain and gamma-gamma dimer remained untouched. It was devoid of plasminogen activation property. It prolonged the activated partial thromboplastin time, prothrombin time and the thrombin clotting time of citrated human plasma. It did not affect the thrombin activity. In mice, defibrinogentaion, prolonged bleeding time (P < 0.01) and reduced fibrinogen level were observed following intravenous injection. Human plasma or alpha2-macroglobulin did not, but the polyvalent anti-venom inhibited the NN-PF3 activity. In contrast to most snake venom metalloproteases, it did not degrade extra cellular matrix proteins.

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“…ED 50 for lethality was estimated by probits (Finney, 1971). For defibrinogenating and in vitro coagulant activities, neutralization was expressed as Effective Dose (ED), as defined by Gené, Roy, Rojas, Gutiérrez and Cerdas (1989) and Segura et al (2010).…”

Section: Neutralization Of Toxic Activitiesmentioning

confidence: 99%

Preclinical efficacy against toxic activities of medically relevant Bothrops sp. (Serpentes: Viperidae) snake venoms by a polyspecific antivenom produced in Mexico

Segura

Herrera

Vargas

et al. 2016

RBT

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Abstract:The assessment of the preclinical neutralizing ability of antivenoms in Latin America is necessary to determine their scope of efficacy. This study was aimed at analyzing the neutralizing efficacy of a polyspecific bothropic-crotalic antivenom manufactured by BIRMEX in Mexico against lethal, hemorrhagic, defibrinogenating and in vitro coagulant activities of the venoms of Bothrops jararaca (Brazil), B. atrox (Perú and Colombia), B. diporus (Argentina), B. mattogrossensis (Bolivia), and B. asper (Costa Rica). Standard laboratory tests to determine these activities were used. In agreement with previous studies with bothropic antivenoms in Latin America, a pattern of cross-neutralization of heterologous venoms was observed. However, the antivenom had low neutralizing potency against defibrinogenating effect of the venoms of B. atrox (Colombia) and B. asper

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Comparative study on coagulant, defibrinating, fibrinolytic and fibrinogenolytic activities of Costa Rican crotaline snake venoms and their neutralization by a polyvalent antivenom

Cited by 186 publications

References 9 publications

Role of nitric oxide in the local and systemic pathophysiological effects induced by Bothrops asper snake venom in mice

Role of nitric oxide in the local and systemic pathophysiological effects induced by Bothrops asper snake venom in mice

Anti-coagulant activity of a metalloprotease: further characterization from the Indian cobra (Naja naja) venom

Preclinical efficacy against toxic activities of medically relevant Bothrops sp. (Serpentes: Viperidae) snake venoms by a polyspecific antivenom produced in Mexico

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