Comparative study on diatom morphology and molecular identification in drowning cases

Liu, Mengyan; Zhao, Yi; Sun, Yuzhe; Li, Yanan; Zhou, Shiliang; Ren, Liang

doi:10.1016/j.forsciint.2020.110552

Cited by 20 publications

(21 citation statements)

References 20 publications

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“…Suitable DNA barcode for DNA metabarcoding Whether a soil sample could be traced back to its original source localities depends on the suitable DNA barcode to be used. Although there is incompatibility between the universality of primers and the resolution of barcodes for higher plants, the universality of primers is more important for DNA metabarcoding of lower plants in soil samples because almost all DNA barcodes are variable enough to resolve most known taxonomic units (Liu et al, 2020b). Lower plants have shorter lifetime, evolve much more quickly and accumulate more genetic variations in their genomes than higher plants.…”

Section: Organism Contamination and False Positivementioning

confidence: 99%

Determination of a criminal suspect using environmental plant DNA metabarcoding technology

Liu

Dong

et al. 2021

Forensic Science International

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Background: There are criminal cases that no frequently used evidence, for example, DNAs from the criminal, is available. Such cases usually are unresolvable. With the advent of DNA metabarcoding, evidences are mined from environmental DNA and such cases become resolvable. This study reports how a criminal suspect was determined by environmental plant DNA metabarcoding technology. A girl was killed in a rural wet area in China without a witness or video record. Pants with dried mud was found from one of her boyfriend's house. The mud was removed from the pants and 11 more mud or soil samples surrounding murder scene were collected. DNA was extracted from the soil. Chloroplast rbcL gene fragments were ampli ed and sequenced on a next generation sequencing platform.Results: After bioinformatics analysis, of the 2980 ZOTUs in total obtained from the 12 samples, 1495 ZOTUs were identi ed to species, genera or families based on the existing public database. The feast analysis based on either taxa or taxa plus abundance data demonstrated that the mud on the suspect's pants was from the criminal scene.Conclusions: The suspect nally made a clean breast of his crime. This case implies that plant DNA in the environment soil is a new source of evidence in determination of suspects using DNA metabarcoding technology and has high potentials of extensive applications in criminal cases.

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Section: Organism Contamination and False Positivementioning

confidence: 99%

Determination of a criminal suspect using environmental plant DNA metabarcoding technology

Liu

Dong

et al. 2021

Forensic Science International

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“…Diagnosis of drowning is one of the major challenges in forensic medicine and is usually performed with pathology and laboratory findings combining different techniques, such as evaluation of fluid electrolyte differences in blood, pleural liquid, and vitreous humor of the victims, analysis of aquaporin expression, investigation of diatoms [101][102][103][104][105][106].…”

Section: Mirnas and Drowningmentioning

confidence: 99%

“…Different molecular methods have been employed to find specific biomarkers of drowning such searching of diatoms DNA in victim's tissues, analysis of intrapulmonary expression of the receptor for advanced glycation end products (RAGE), aquaporin-5 (AQP5), surfactant protein-A (SP-A), interleukin 6 (IL-6) and interleukin 1β (IL-1β) as markers of drowning [103][104][105][106].…”

Section: Mirnas and Drowningmentioning

confidence: 99%

MicroRNAs: An Update of Applications in Forensic Science

et al. 2020

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MicroRNAs (miRNAs) are a class of non-coding RNAs containing 18–24 nucleotides that are involved in the regulation of many biochemical mechanisms in the human body. The level of miRNAs in body fluids and tissues increases because of altered pathophysiological mechanisms, thus they are employed as biomarkers for various diseases and conditions. In recent years, miRNAs obtained a great interest in many fields of forensic medicine given their stability and specificity. Several specific miRNAs have been studied in body fluid identification, in wound vitality in time of death determination, in drowning, in the anti-doping field, and other forensic fields. However, the major problems are (1) lack of universal protocols for diagnostic expression testing and (2) low reproducibility of independent studies. This review is an update on the application of these molecular markers in forensic biology.

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“…In recent times, many studies have shown the advantages of DNA barcoding combined with High-Throughput Sequencing (HTS) methods, compared to conventional approaches [3][4][5][6][7], and thus DNA-based monitoring has been postulated as a promising tool in recent years for the assessment of aquatic ecosystems [8][9][10][11][12]. The development of high-throughput techniques have made this process quicker and easier, and now there are studies available evaluating its application in the biomonitoring of rivers [8][9][10][11][13][14][15] and large lakes [16].…”

Section: Introductionmentioning

confidence: 99%

Epiphytic Diatom-Based Biomonitoring in Mediterranean Ponds: Traditional Microscopy versus Metabarcoding Approaches

Borrego-Ramos

Bécares

Garcı́a

et al. 2021

Water

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Benthic diatoms have traditionally been used as bioindicators of aquatic ecosystems. Because diatom-based monitoring of water quality is required by European legislation, molecular-based methods had emerged as useful alternatives to classical methods based on morphological identification using light microscopy. The aim of this study was to test the reliability of DNA metabarcoding combined with High-Throughput Sequencing (HTS) techniques in the bioassessment of the trophic status of 22 Mediterranean shallow ponds in NW Spain. For each pond, the Trophic Diatom Index (TDI) was calculated from inventories obtained by identification using light microscopy (LM) followed by high-throughput sequencing (HTS) at the molecular level. Ponds were subsequently classified into five water quality classes. The results showed a good correspondence between both methods, especially after applying a correction factor that depended on the biovolume of the cells. This correspondence led to the assignment to the same quality class in 59% of the ponds. The determination and quantification of valves or DNA sequences was one of the main pitfalls, which mainly included those related to the variability in the relative abundances of some species. Accordingly, ponds with similar relative abundances for the dominant species were assigned to the same quality class. Moreover, other difficulties leading the discrepancies were the misidentification of some species due to the presence of semi-cryptic taxa, the incompleteness of the reference database and the bioinformatic protocol. Thus, the validation of DNA-based methods for the identification of freshwater diatoms represents an important goal, as an alternative to using traditional methods in Mediterranean shallow ponds.

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Comparative study on diatom morphology and molecular identification in drowning cases

Cited by 20 publications

References 20 publications

Determination of a criminal suspect using environmental plant DNA metabarcoding technology

Determination of a criminal suspect using environmental plant DNA metabarcoding technology

MicroRNAs: An Update of Applications in Forensic Science

Epiphytic Diatom-Based Biomonitoring in Mediterranean Ponds: Traditional Microscopy versus Metabarcoding Approaches

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