Comparative study on factors regulating the level of oxidative stress toward pulmonary nuclei in two different strains of mice

Yano, Tomohiro; Ishikawa, Gaku; Ichikawa, Tomio

doi:10.1016/0020-711x(94)90204-6

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…The mitochondrial fraction was suspended in 0.2 M phosphate buffer (pH 7.4) and washed 3 times. To measure superoxide anion/hydrogen peroxide generated the mitochondrial fraction, lucigenin-amplified chemiluminescence was assayed with a luminescence reader (Aloka, Tokyo, Japan) [13]. The assay mixture for chemiluminescence contained 0.2 m M lucigenin, 0.2 m M NADH, 0.1 mg protein and 0.1 M phosphate buffer (pH 7.4), and the reaction was performed at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Activation of Epidermal Growth Factor Receptor in the Early Phase after Renal Ischemia-Reperfusion in Rat

Yano¹,

Yazima²,

Hagiwara³

et al. 1999

Nephron

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In order to estimate a regenerative response in the early phase after renal ischemia-reperfusion in rat, we examined the time course of the activation of epidermal growth factor receptor (EGFR) as a response of signal transduction pathway after 45 min ischemia in kidney. The activation of EGFR was observed 5–30 min after the start of reperfusion. Simultaneously, superoxide anion/hydrogen peroxide generated in the mitochondrial fraction was elevated during the same period. On the other hand, the level of EGF decreased in a time-dependent manner. These results suggested that superoxide anion/hydrogen peroxide generated during the ischemia-reperfusion other than EGF could act as an activator for the EGFR. In summary, the activation of EGFR is important as a regenerative response at an early stage after the start of reperfusion in ischemic kidney.

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Section: Methodsmentioning

confidence: 99%