Comparative study on influence of fetal bovine serum and serum of adult rat on cultivation of newborn rat neural cells

Sukach, A. N.; Shevchenko, M. V.; Liashenko, T. D.

doi:10.7124/bc.0008b7

Cited by 4 publications

(4 citation statements)

References 10 publications

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“…The experiments were carried out in accordance with the «General principles of animal experiments» approved by the V Congress on Bioethics (Kyiv, 2013) and the «European Convention for the Protection of Vertebrate Animals used for experimental and other scientific purposes» (Strasbourg, 1986) Culture of neural cells. NCs were isolated from the brain of newborn Wistar rats on the first day after their birth by a previously developed method [14]. For this purpose, the tissue was disaggregated for 15 minutes in 0.25 % trypsin solution (BioWest, France) on phosphate buffered saline (PBS) at 37 °C.…”

Section: Resultsmentioning

confidence: 99%

Placental stem cells, organotypic culture and human placenta extract have neuroprotective activity in vitro

Prokopіuk¹,

Chub²,

Shevchenko³

et al. 2017

JCOT

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According to WHO, 6.7 million people die from stroke every year. The search for new neuroprotective substances remains an urgent task.The purpose of this study was to investigate the neuroprotective activity of factors of placental origin.Materials and methods. Neuroprotective activity of media conditioned with cryopreserved placenta derived mesenchymal stem cells (MSCs), organotypic culture of placenta and placental extract was studied on in vitro models of glutamate excitotoxicity in rats` neural cells. Neural cells were cultured with placental factors without glutamate treatment, before and after glutamate treatment. Neural cells` metabolic activity was assessed by MTT test.Results. Placental factors increase the MTT test indexes, prevent the toxic effect of glutamate on neural cells and promote their recovery. The thermolability of factors of placental origin and the effectiveness of various placental preparations are shown.Conclusions. Conditional media of placenta derived MSCs, organotypic culture of the placenta and human placental extract have neuroprotective effect on rats` brain cells in vitro.

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Section: Resultsmentioning

confidence: 99%

Placental stem cells, organotypic culture and human placenta extract have neuroprotective activity in vitro

Prokopіuk¹,

Chub²,

Shevchenko³

et al. 2017

JCOT

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“…When isolated by the enzymatic-mechanical method, the brain tissue was washed from blood, then transferred into a trypsin-versene solution and incubated at 37 °C for 2 minutes. After that, the tissue was transferred into DMEM/F12 supplemented with 10 % adult rat blood serum (ARBS) [8] and mechanically disaggregated into single cells using vibration [7]. The resulting suspension was filtered through a nylon filter and then washed from trypsin by centrifugation at 100 g for 2 min.…”

Section: Methodsmentioning

confidence: 99%

“…Cells were cultured in 24-well plates (Corning, USA) at concentrations of 0.2|4×10 6 cells/ml in DMEM/F12 supplemented with 0.6 % glucose and 2 mM glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, in the presence of or without 10 % ARBS [8]. The cells were cultivated in a CO 2 incubator at 37 °C in an atmosphere of 5 % CO 2 , 95 % air.…”

Section: Methodsmentioning

confidence: 99%

Obtaining and characterization of rat fetuses neural cells aggregates/spheroids

et al. 2022

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Search for optimal conditions for the formation of aggregates/spheroids by neural cells (NC) isolated from the brain tissue of rat fetuses. Study of behavioral characteristics of NC in culture as a part of three-dimensional aggregates/spheroids. Methods. Isolation of NC from fetal rat brain tissue; cell viability assessment; cultivation; immunocytochemical staining; NC proliferative activity assessment. Results. It was found that the conditions for the robust formation of aggregates are: 1) the presence of 10 % adult rat's blood serum in the cell culture medium, 2) the seeding cell density -1-4×10 6 cells/ml and 3) the initial cell viability higher than 20 %. Conclusions. The isolated rat fetuses brain cells, when cultured in the presence of 10 % serum and at concentrations above 1×10 6 cells/ml, spontaneously form multicellular aggregates. The structure of aggregates formed during short-term cultivation depends on the initial cell viability, and the size of aggregates depends on the initial viability and seeding densities of NC. The rat fetuses NC aggregates/spheroids contain stem/progenitor cells that can proliferate and differentiate. NC aggregates/spheroids form three-dimensional structures, in which favorable conditions are developed for the survival and adequate functioning of the committed and stem/progenitor NC.

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“…For aggregates formation NCs were plated in 24-well plastic plate (Corning, USA) at a concentration of 4•10 6 cells per well and cultured for 2 hours in 0.5 mL supplemented DMEM/F12 with 10 % ARS [9]. Cells were resuspended with a pipette every 15 minutes.…”

Section: Methodsmentioning

confidence: 99%

Transplantation of cryopreserved rat fetal neural cells in suspension and in multicellular aggregates into rats with spinal cord injury

Sukach¹,

Lebedinsky²,

Ochenashko³

et al. 2016

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Today cell transplantation is one of the promising approaches of spinal cord injuries treatment. The aim of the work was to study the effect of cryopreserved fetal neural cell transplantation in suspensions and cell aggregates for motor activity recovery of rats with experimental spinal cord injury. MATERIALS AND METHODS. Cells were isolated from the brain tissue of rat fetuses 15-16 days of gestation. The formation of aggregates was performed during short-term cultivation at a concentration of 8•106 cells/mL in medium with 10 % adult rat serum. Cell transplantation was performed into the damaged area of spinal cord in aggregates or suspension. To fix transplanted cells in the damaged area we used alginate gel. RESULTS. Transplantation of cryopreserved fetal neural cells in alginate gel

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Comparative study on influence of fetal bovine serum and serum of adult rat on cultivation of newborn rat neural cells

Cited by 4 publications

References 10 publications

Placental stem cells, organotypic culture and human placenta extract have neuroprotective activity in vitro

Placental stem cells, organotypic culture and human placenta extract have neuroprotective activity in vitro

Obtaining and characterization of rat fetuses neural cells aggregates/spheroids

Transplantation of cryopreserved rat fetal neural cells in suspension and in multicellular aggregates into rats with spinal cord injury

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