Comparative study on larvicidal activity of green synthesized silver nanoparticles and Annona glabra (Annonaceae) aqueous extract to control Aedes aegypti and Aedes albopictus (Diptera: Culicidae)

Abstract

The present study reports mosquito larvicidal potential of green synthesized silver nanoparticles by using Annona glabra leaves (An-AgNPs). Synthesized An-AgNPs were characterized by Ultraviolet-Visible spectroscopy (UV-VIS), Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS) technique and Fourier transform infrared spectroscopy (FTIR). Colur change from pale yellow to brick red of the plant extract and AgNO 3 solution indicated the formation of An-AgNPs initially. Surface Plasmon Resonance (SPR) band at 435 nm in the UV-Vis confirmed the formation of An-AgNPs. SEM images showed that An-AgNPs were spherical in shape. FTIR proved that An-AgNPs were functionalized with biomolecules in A. glabra leaves. Based on DLS analysis the average size range of synthesized An-AgNPs was determine to be 10-100 nm and 100-1000 nm.Third instar larvae of dengue vector mosquitoes, Aedes aegypti and Aedes albopictus were subjected to larvicidal bioassays in a range of concentrations of An-AgNPs and A. glabra crude aqueous leaf extract (2-10 mg/L). An-AgNPs exhibited very high larvicidal activity against dengue vector mosquito larvae; LC 50 value for Ae. aegypti at 24 h exposure to An-AgNPs (Plant extract: AgNO 3 1 : 10) 5.29 mg/L; An-AgNPs (Plant extract: AgNO 3 2 : 10) 2.43 mg/L while LC 50 value for Ae. albopictus at 24 h exposure to An-AgNPs (Plant extract: AgNO 3 1:10) 3.02 mg/ L; An-AgNPs (Plant extract: AgNO 3 2:10) 2.51 mg/L. LC 50 values obtained for A. glabra leaf extract tested against Ae. aegypti and Ae. albopictus are 5.94 mg/L and 5.00 mg/L respectively at 24-hour exposure. This study further revealed that Ae. albopictus is more susceptible than to Ae. aegypti to a given concentration of An-AgNPs and to crude aqueous leaf extract of A. glabra. Larvicidal effect of An-AgNPs is superior to the crude aqueous leaf extract of A. glabra. An-AgNPs is a potent larvicide for dengue vector control.

An Updated Review on Silver Nanoparticles in Biomedicine

“…Nanosilver-based formulations proved efficient therapeutic effects against several pathologies caused by clinically-relevant viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) [ 211 , 212 ], human papilloma virus (HPV) [ 213 ], rotavirus [ 214 , 215 ] and other enteric viruses [ 216 , 217 , 218 ]. It is worth mentioning that new and effective platforms containing AgNPs were evaluated for their biocidal activity against viral vectors, generally mosquito-borne pathogens including Zika virus [ 219 , 220 ], Dengue virus [ 221 , 222 ], West Nile virus [ 223 , 224 ] and Chikungunya virus [ 225 , 226 ].…”

Biogenic Synthesis of Silver Nanoparticles Mediated by the Consortium Comprising the Marine Fungal Filtrates of Penicillium Oxalicum and Fusarium Hainanense Along With Their Antimicrobial, Antioxidant and Larvicidal Potency

“…Furthermore, AgNPs can easily penetrate into the exoskeleton of larvae and attach to the sulfur or phosphorus containing biomolecules like proteins, DNA and RNA which leads to the quick denaturation of organelles and enzymes which could be the possible reason for the death of mosquito larvae. Due to the disturbance in proton motive force important for ATP construction and decreased permeability of membrane by AgNPs, cellular functions may not work properly and cell will die [31,32,33]. AgNPs synthesized from DSW-28 had shown highest rate of mortality against both the larvae.…”

Biosynthesis of Silver Nanoparticles Mediated by Entomopathogenic Fungi: Antimicrobial Resistance, Nanopesticides, and Toxicity

“…Thus, metal and metal-oxide nanoparticles are being studied as materials with high potential for pest control [4,7,8]. Emphasis has been put on silver nanoparticles (AgNPs) for Antibiotics 2021, 10, 852 2 of 13 their easy synthesis, which can be performed by different methods and reducing/stabilizing agents, and versatility of application, due to their physical-chemical characteristics and toxicity on several insects [4,6,[9][10][11]. Currently, biological synthesis has been extensively explored for the production of AgNPs because it is sustainable, with high reproducibility and low production cost.…”