Comparative Study on Remineralizing Ability of Casein Phosphopeptide Amorphous Calcium Phosphate and β-Tricalcium Phosphate on Dental Erosion: An In vitro Atomic Force Microscope Study

Abstract

reflects the bacterial adhesion and plaque formation on the surface of tooth. This study basically intends to compare the remineralizing potential of the dentifrices containing recently invented CPP-ACP and β-TCP on dental erosion produced by soft drinks on enamel using AFM. Materials and Methods A total of 40 extracted human anterior teeth were collected for the study. Teeth were cleaned by ultrasonic scaler to remove soft debris and inspected for cracks, hypoplasia or any white spot lesions. Further disinfection was carried out by immersing them in 5% sodium hypochlorite for 1 hour and storing in de-ionized water. The specimens were decoronated and sliced longitudinally for the labial portion using diamond disc with water irrigation. The labial surface of each enamel specimen grounded using silicon carbide papers with consecutive 600, 1000 and 1200 grits under water irrigation for producing a flat surface. The CPP-ACP paste used was GC Tooth mousse (GC Corp, Tokyo, Japan) and β-TCP paste was Clinpro TM Tooth Crèam (3M ESPE, USA) (Figure 1). The pH of soft drink was determined by pH meter. All the samples were randomly divided into two groups. Group